A5070640549- Influenza Virus Research Studies
- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Lipid Membrane Structure and Behavior
- Advanced Optical Sensing Technologies
- Cellular transport and secretion
- Virus-based gene therapy research
- Microtubule and mitosis dynamics
- Respiratory viral infections research
- Genetics, Bioinformatics, and Biomedical Research
- Diabetes and associated disorders
- Monoclonal and Polyclonal Antibodies Research
- Force Microscopy Techniques and Applications
- AI in cancer detection
- Pancreatic function and diabetes
- Scientific Measurement and Uncertainty Evaluation
- Spectroscopy Techniques in Biomedical and Chemical Research
- Healthcare cost, quality, practices
- DNA Repair Mechanisms
- Diabetes Management and Research
- COVID-19 and healthcare impacts
- Molecular Biology Techniques and Applications
- Fungal and yeast genetics research
- Reliability and Agreement in Measurement
Max Planck Institute of Molecular Cell Biology and Genetics
2009-2023
European Molecular Biology Organization
2023
German BioImaging – Gesellschaft für Mikroskopie und Bildanalyse
2020
Max Planck Society
2008-2009
Humboldt-Universität zu Berlin
1995-2002
Freie Universität Berlin
1995
In a motile eukaryotic cell, front protrusion and tail retraction are superimposed on each other. To single out mechanisms that result in to or transition, we separated the two processes time using cells oscillate between full state. State transitions were visualized by total internal reflection fluorescence microscopy as marker PIP3 (phosphatidylinositol [3,4,5] tris-phosphate), tumor-suppressor PTEN (phosphatase tensin homolog) degrades PIP3. Negative fluctuations layer of membrane gated...
Actin waves that travel on the planar membrane of a substrate-attached cell underscore capability actin system to assemble into dynamic structures by recruitment proteins from cytoplasm. The have no fixed shape, can reverse their direction propagation, and fuse or divide. separate two phases plasma are distinguished lipid composition. area circumscribed wave resembles in its phosphoinositide content interior phagocytic cup, leading us explore possibility in-plane generated without localized...
Insulin is stored within the secretory granules of pancreatic β-cells, and impairment its release hallmark type 2 diabetes. Preferential exocytosis newly synthesized insulin suggests that granule aging a key factor influencing secretion. Here, we illustrate technology enables study in insulinoma cells β-cells knock-in mice through conditional unequivocal labeling fused to SNAP tag. This approach, which overcomes limits encountered with previous strategies based on radiolabeling or...
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Propagating actin waves are dynamic supramolecular structures formed by the self‐assembly of proteins within living cells. They built from filaments together with single‐headed myosin, Arp2/3 complex, and coronin in a defined three‐dimensional order. The function these structuring cell cortex is studied on substrate‐attached surface Dictyostelium cells use total internal reflection fluorescence "TIRF" microscopy. Actin separate two areas each other, which distinguished arrangement filaments....
In a motile polarized cell the actin system is differentiated to allow protrusion at front and retraction tail. This differentiation linked phosphoinositide pattern in plasma membrane. highly Dictyostelium cells studied here, dominated by PI3-kinases producing PI(3,4,5)tris-phosphate (PIP3), tail PI3-phosphatase PTEN that hydrolyses PIP3 PI(4,5)bis-phosphate. To study de-novo polarization, we first depolymerized subsequently recorded spontaneous reorganization of patterns relation PTEN....
ABSTRACT The fusion activity of chimeras influenza virus hemagglutinin (HA) (from A/fpv/Rostock/34; subtype H7) with the transmembrane domain (TM) and/or cytoplasmic tail (CT) either from nonviral, nonfusogenic T-cell surface protein CD4 or fusogenic Sendai F-protein was studied. Wild-type chimeric HA expressed in CV-1 cells by transient T7-RNA-polymerase vaccinia expression system. Subsequently, products monitored red blood ghosts as target cells. To assess different steps fusion, were...
ABSTRACT The role of the sequence transmembrane and cytoplasmic/intraviral domains influenza virus hemagglutinin (HA, subtype H7) for HA-mediated membrane fusion was explored. To analyze influence two on fusogenic properties HA, we designed HA-chimeras in which cytoplasmic tail and/or domain HA replaced with corresponding glycoprotein F Sendai virus. These chimeras, as well constructs by peptides human neurofibromin type1 (NF1) or c-Raf-1, NF78 (residues 1441 to 1518), Raf81 51 131),...
The spreading of motile cells on a substrate surface is accompanied by reorganization their actin network. We show that in the highly Dictyostelium non-monotonic, and thus differs from passage through regular series stages. Quantification gain loss contact area revealed fluctuating forces protrusion retraction dominate interaction with substrate. molecular basis these fluctuations elucidated dual-fluorescence labeling filamentous together proteins highlight specific activities system....
Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging particular is currently undergoing rapid technological advancements. However for scientists wishing to publish the obtained images image analyses results, there date no unified guidelines. Consequently, microscopy data publications may be unclear or difficult interpret. Here we present community-developed checklists preparing light analysis publications. These offer authors, readers,...
Operating shared resource laboratories (SRLs) in times of pandemic is a challenge for research institutions. In multiuser, high-turnover working space, the transmission infectious agents difficult to control. To address this challenge, imaging core facility managers being members German BioImaging discussed how microscopes could be operated with minimal risk spreading SARS-CoV-2 between users and staff. Here, we describe resulting guidelines explain their rationale, focus on separating space...
The protocols in this collection describe how to measure and analyze the photon conversation factor (PCF photo-electrons/count), readnoise, dynamic range of a light microscopy detection system; which can either be point detector or an area detector, using in-homogeneous illumination scheme (as opposed uniform illumination). includes on prepare suitable sample for acquisition, acquire data, as well respective analysis protocol. There are three aims system characterized for, briefly: Aim 1 -...
This protocol is the introduction to protocols collection "Characterization of Photon Conversion Factor, Dark Noise, and Dynamic Range Light Microscope Detection Systems". Here we describe in more detail mission statement QUAREP-LiMi Working Group 2, different aims detection system characterization, a short guide collection, basic theory underlying this collection.
The aim of this analysis is to obtain various calibration metrics from inhomogenous images using the photon transfer method (McFadden et al., 2022 and Heintzmann 2016). not completely automated, relies on users interpret data, identify problems scrutinize results. It generally unaware underlying detector technology, which can affect This guide therefore intends provide an overview method, choices that a user must make, how common pitfalls should be avoided. Note significantly differs other...
To obtain accurate, reproducible, and interpretable data when conducting imaging experiments, it is critical to consider external factors affecting acquisition at various steps of the experimental workflow. Illumination power stability represent two factors, especially comparing fluorescence intensities between images during a time-lapse experiment or experiments performed different times on microscopes. The signal can be generated by types light sources. These sources their coupling...
This protocol describes the measurement procedure to produce dark and inhomogeneously illuminated images with a light microscope system equipped area detectors. The can be followed according three different aims of characterization described in introduction collection "Characterization Photon Conversion Factor, Noise, Dynamic Range Light Microscope Detection Systems". uses slide for inhomogeneous illumination 2 generates data which analyzed 5, obtain photon conversion factor, readnoise,...
This protocol describes how to create a high dynamic range fluorescent sample using basic office and microscopy lab tools. The is based on the usage of ink text marker thin layer microscope cover glass (Olevsko et al., 2021). result carrier with thin, homogeneous film abrupt edges thickness in order few micrometers. type can be used make images smooth gradient going from maximum intensity background, as described protocols for determination detector’s photon conversion factor.
This protocol describes the measurement procedure to produce dark and inhomogeneous illuminated images with a scanning light microscope system equipped point detectors. The can be followed according three different aims of characterization described in introduction collection "Characterization Photon Conversion Factor, Noise, Dynamic Range Light Microscope Detection Systems". uses slide for illumination 2 generates data which analyzed 5, obtain photon conversion factor, readnoise, dynamic...