A5040881319- Cell Image Analysis Techniques
- Zebrafish Biomedical Research Applications
- AI in cancer detection
- Retinal Development and Disorders
- Genetics, Bioinformatics, and Biomedical Research
- ATP Synthase and ATPases Research
- Axon Guidance and Neuronal Signaling
- Hippo pathway signaling and YAP/TAZ
- Ubiquitin and proteasome pathways
- Genetics and Neurodevelopmental Disorders
- Angiogenesis and VEGF in Cancer
- Microtubule and mitosis dynamics
- Phagocytosis and Immune Regulation
- Epigenetics and DNA Methylation
- Electrospun Nanofibers in Biomedical Applications
- Photoreceptor and optogenetics research
- Image Processing Techniques and Applications
- Cancer-related Molecular Pathways
- Tissue Engineering and Regenerative Medicine
- Mitochondrial Function and Pathology
- Biomedical and Engineering Education
- Radiomics and Machine Learning in Medical Imaging
- Cancer Research and Treatments
- Tracheal and airway disorders
- Pancreatic and Hepatic Oncology Research
Karolinska Institutet
2007-2025
European Molecular Biology Organization
2023
Temasek Life Sciences Laboratory
2004
Institute of Molecular and Cell Biology
2002
Abstract Deep Convolutional Neural Networks (DCNN) have recently emerged as superior for many image segmentation tasks. The DCNN performance is however heavily dependent on the availability of large amounts problem-specific training samples. Here we show that DCNNs trained ground truth created automatically using fluorescently labeled cells, perform similar to manual annotations.
Visual system development is dependent on correct interpretation of cues that direct growth cone migration and axon branching. Mutations in the zebrafish esrom gene disrupt bundling targeting retinal axons, also cause ectopic arborization. By positional cloning, we establish encodes a very large protein orthologous to PAM (protein associated with Myc)/Highwire/RPM-1. Unlike motoneurons Drosophila highwire mutants, axons mutants do not arborize excessively, indicating Esrom has different...
The low reproducibility of scientific data published in articles has recently become a cause concern many fields. Data involving light microscopy is no exception. awareness researchers the technologies they use their research been identified as one main causes problem. Potential solutions have hinted at need to improve technological and methodological education within research. Despite pivotal role core facilities being well documented, facility staff (FS) often learn trade on job, without...
Tumor-residing microbiota poses a new challenge in cancer progression and therapy; however, the functional behavior of patient tumor-derived microbes remains poorly understood. We previously reported presence tumor intraductal papillary mucinous neoplasms (IPMNs), which are precursors pancreatic cancer. examined metabolic pathogenic potential clinical strains obtained from IPMN tumors using various cell lines 3D spheroid models. Our findings revealed that several IPMNs with invasive or...
Cell anchorage is required for cell proliferation of untransformed cells, whereas anchorage-independent growth can be induced by oncogenes and a hallmark transformation. Whereas anchorage-dependent control the progression G 1 phase cycle has been extensively studied, it less clear whether how may other phases affect such controls. Here, we found that lack did not influence through S phase, 2 or most mitosis primary human fibroblasts. However, unanchored fibroblasts could complete...
Members of the Eph-B family receptors tyrosine kinase and their transmembrane ligands have been implicated in dorsoventral patterning vertebrate retinotectal projection. In zebrafish system, however, ephrinB2a is expressed strongly posterior tectum, tectal neurons that form physical contacts with retinal ganglion cell (RGC) axons. gnarled mutant, where ectopically pretectum, RGC axons stall before entering or else are misrouted branch aberrantly neuropil. Ectopic expression anterior midbrain...
Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging particular is currently undergoing rapid technological advancements. However for scientists wishing to publish the obtained images image analyses results, there date no unified guidelines. Consequently, microscopy data publications may be unclear or difficult interpret. Here we present community-developed checklists preparing light analysis publications. These offer authors, readers,...
We have identified two simple methods to analyse xanthophore and pterinosome biogenesis in zebrafish. The first uses methylene blue (methylthionium chloride), a redox dye which specifically labels xanthophores pterinosomes, while the second autofluorescence detect pteridine levels; these may be used number, location shape of pterinosomes. These assays were applied zebrafish mutants – brie yobo revealed that both synthesis defects. Additionally, using capillary electrophoresis, we provide...