A5082642221- Adenosine and Purinergic Signaling
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Nanoplatforms for cancer theranostics
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Cellular Mechanics and Interactions
- bioluminescence and chemiluminescence research
- Photoacoustic and Ultrasonic Imaging
- Cell Adhesion Molecules Research
- 3D Printing in Biomedical Research
- Medical Imaging Techniques and Applications
- Adrenal and Paraganglionic Tumors
- Smart Materials for Construction
- Cancer Cells and Metastasis
- Radiomics and Machine Learning in Medical Imaging
- Adhesion, Friction, and Surface Interactions
- Dermatologic Treatments and Research
- Nanofabrication and Lithography Techniques
- Engineering and Material Science Research
- Photoreceptor and optogenetics research
- Orthopedic Infections and Treatments
- Advanced Physical and Chemical Molecular Interactions
- Optical Imaging and Spectroscopy Techniques
- AI in cancer detection
Radboud Institute for Molecular Life Sciences
2014-2023
Radboud University Nijmegen
2011-2023
Radboud University Medical Center
2012-2023
University Medical Center
2016-2022
Ball (France)
2014
National Superconducting Cyclotron Laboratory
2014
Michigan State University
2014
Moscow Engineering Physics Institute
2014
Institute for Bioengineering of Catalonia
2009-2012
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2012
Cancer cell invasion is an adaptive process based on cell-intrinsic properties to migrate individually or collectively, and their adaptation encountered tissue structure acting as barrier providing guidance. Whereas molecular physical mechanisms of cancer are well-studied in 3D vitro models, topographic relevance, classification validation toward interstitial organization vivo remain incomplete. Using combined intravital third second harmonic generation (THG, SHG), three-channel fluorescence...
Cancer invasion into an extracellular matrix (ECM) results from a biophysical reciprocal interplay between the expanding cancer lesion and tissue barriers imposed by adjacent microenvironment. In vivo, connective provides both densely packed ECM to channel/track-like spaces loosely organized zones, of which may impact mode efficiency; however little is known about how three-dimensional (3D) aligned tracks present in interstitial guide cell invasion. We here describe two-photon laser ablation...
Three-photon excitation has recently been demonstrated as an effective method to perform intravital microscopy in deep, previously inaccessible regions of the mouse brain. The applicability 3-photon for deep imaging other, more heterogeneous tissue types much less explored. In this work, we analyze benefit high-pulse-energy 1 MHz pulse-repetition-rate infrared near 1300 and 1700 nm in-depth tumorous bone tissue. We show that regime provides a than 2-fold increased depth tumor compared...
Integrins are cell membrane adhesion receptors involved in morphogenesis, immunity, tissue healing, and metastasis. A central, yet unresolved question regarding the function of integrins is how these regulate both their conformation dynamic nanoscale organization on to generate adhesion-competent microclusters upon ligand binding. Here we exploit high spatial (nanometer) accuracy temporal resolution single-dye tracking dissect relationship between conformational state, lateral mobility,...
Abstract The tumour microenvironment (TME) forms a major obstacle in effective cancer treatment and for clinical success of immunotherapy. Conventional co-cultures have shed light onto multiple aspects immunobiology, but they are limited by the lack physiological complexity. We develop human organotypic skin melanoma culture (OMC) that allows real-time study host-malignant cell interactions within multicellular tissue architecture. By co-culturing decellularized dermis with keratinocytes,...
Abstract Sphingolipids are essential constituents of the plasma membrane (PM) and play an important role in signal transduction by modulating clustering dynamics receptors. Changes lipid composition therefore likely to influence receptor organisation function, but how this precisely occurs is difficult address given intricacy PM lipid-network. Here, we combined biochemical assays single molecule dynamic approaches demonstrate that local environment regulates adhesion integrin receptors...
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Cell migration research has become a high-content field. However, the quantitative information encapsulated in these complex and high-dimensional datasets is not fully exploited owing to diversity of experimental protocols non-standardized output formats. In addition, typically are open for reuse. Making data Findable, Accessible, Interoperable, Reusable (FAIR) will enable meta-analysis, integration, mining. Standardized formats controlled vocabularies essential building suitable...
The interstitial tumor microenvironment is composed of heterogeneously organized collagen-rich porous networks as well channel-like structures and interfaces which provide both barriers guidance for invading cells. Tumor cells 3D random collagen depend upon actomyosin contractility to deform translocate the nucleus, whereas Rho/Rho-associated kinase-dependent largely dispensable migration in stiff capillary-like confining microtracks. To investigate whether this dichotomy dependence also...
High-content image-based cell phenotyping provides fundamental insights into a broad variety of life science disciplines. Striving for accurate conclusions and meaningful impact demands high reproducibility standards, with particular relevance high-quality open-access data sharing meta-analysis. However, the sources degree biological technical variability, thus usefulness meta-analysis results from live-cell microscopy, have not been systematically investigated. Here, using high-content...
Cytotoxic T lymphocytes (CTL) are antigen-specific effector cells with the ability to eradicate cancer in a contact-dependent manner. Metabolic perturbation compromises CTL response tumor subregions, resulting failed cell elimination despite infiltration of tumor-specific CTLs. Restoring functionality these tumor-infiltrating CTLs is key improve immunotherapy. Extracellular adenosine an immunosuppressive metabolite produced within microenvironment. Here, by applying single-cell reporter...
Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging particular is currently undergoing rapid technological advancements. However for scientists wishing to publish the obtained images image analyses results, there date no unified guidelines. Consequently, microscopy data publications may be unclear or difficult interpret. Here we present community-developed checklists preparing light analysis publications. These offer authors, readers,...
Ligand-patterned surfaces alter the spatio-temporal organization of specific receptors on cell membrane. Chemically confined are fabricated using microcontact patterning. The dynamic re-organization integrin LFA-1 in living cells is monitored at single-molecule level total internal reflection fluorescence. image left shows individual nanoclusters a single being recruited to ligand-rich areas pattern.
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Abstract Cell migration research has become a high-content field. However, the quantitative information encapsulated in these complex and high-dimensional datasets is not fully exploited due to diversity of experimental protocols non-standardised output formats. In addition, typically are open for reuse. Making data Findable, Accessible, Interoperable, Reusable (FAIR) will enable meta-analysis, integration, mining. Standardised formats controlled vocabularies essential building suitable...