A5025726249- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Zebrafish Biomedical Research Applications
- Renal and related cancers
- Photoacoustic and Ultrasonic Imaging
- Saffron Plant Research Studies
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Phytochemicals and Antioxidant Activities
- Heme Oxygenase-1 and Carbon Monoxide
- Machine Learning in Materials Science
- Medicinal Plants and Bioactive Compounds
- Neuroscience and Neural Engineering
- Optical Coherence Tomography Applications
- Flavonoids in Medical Research
- Poisoning and overdose treatments
- Photoreceptor and optogenetics research
- Molecular Biology Techniques and Applications
- Hemoglobin structure and function
- Bee Products Chemical Analysis
TU Dresden
2020-2023
Center for Systems Biology Dresden
2021-2023
University of Florence
2017
Leibniz Institute of Photonic Technology
2014
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Morphogen gradients impart positional information to cells in a homogenous tissue field. Fgf8a, highly conserved growth factor, has been proposed act as morphogen during zebrafish gastrulation. However, technical limitations have so far prevented direct visualization of the endogenous Fgf8a gradient and confirmation its morphogenic activity. Here, we monitor propagation developing neural plate using CRISPR/Cas9-mediated EGFP knock-in at fgf8a locus. By combining sensitive imaging with...
Carbon monoxide (CO) is a toxic gas for mammals, and despite this fact, it naturally produced in these organisms has been proven to be beneficial medical treatments, too. Therefore, CO-releasing molecules (CORMs) are intensively developed administer dose CO physiological applications. Nearly all of compounds metal carbonyl complexes, which have synthesized investigated. However, most CORMs, the exact reaction mechanisms release not completely elucidated, although utmost importance. The...
Light-sheet microscopy (LSM), in combination with intrinsically transparent zebrafish larvae, is a choice method to observe brain function high frame rates at cellular resolution. Inherently LSM, however, residual opaque objects cause stripe artifacts, which obscure features of interest and, during functional imaging, modulate fluorescence variations related neuronal activity. Here, we report how Bessel beams reduce streaking artifacts and produce high-fidelity quantitative data...
Age-related macular degeneration (AMD) is a leading cause for visual impairment in aging populations with limited established therapeutic interventions available. Oxidative stress plays an essential role the pathogenesis of AMD, damaging retinal pigment epithelium (RPE), which function and maintenance light-sensing photoreceptors. This study aimed to evaluate effects crocetin, one main components Saffron, on vitro RPE model tert-butyl hydroperoxide (TBHP) induced oxidative using ARPE19...
Abstract Morphogen gradients impart positional information to cells in a homogenous tissue field. Fgf8a, highly conserved growth factor, has been proposed act as morphogen during zebrafish gastrulation. However, technical limitations have so far prevented direct visualization of the endogenous Fgf8a gradient and confirmation its morphogenic activity. Here, we monitored propagation developing neural plate using CRISPR/Cas9-mediated EGFP knock-in at fgf8a locus. By combining sensitive imaging...
Bessel beam have attractive properties like their propagation invariance and "self-healing" capabilities. Here we present significant advantages of illumination in structural functional imaging light-sheet microscopy compared to conventional Gaussian illumination.
Light-sheet microscopy (LSM) has proven a useful tool in neuroscience and is particularly well suited to image the entire brain with high frame rates at single cell resolution. On one hand, LSM employed combination tissue clearing methods like CLARITY which allows for reconstruction of neuronal or vascular anatomy over cm-sized samples. other been paired intrinsically transparent samples real-time recording activity resolution across brain, using calcium indicators GCaMP6. Despite its...
The authors wish to make the following correction Figure 6B of this article [...]
One of the most exciting challenges neurosciences in last few years is real-time recording neuronal activity with single cell resolution across entire brain. Thanks to use optical methods, together animal models which whole encephalon optically accessible, this goal getting within reach. In work, we a transgenic zebrafish line expressing genetically encoded calcium indicator GCaMP6s binding ions leads an increase emitted fluorescence reporter. sensitive reporter family and allows us record...
The large number of neurons and neural interconnection makes the nervous system a highly dense complex network. Understanding functionality such network requires not only high-throughput recording activities with cellular resolution but also non-invasive precisely defined interaction neurons. This work demonstrates two pivotal steps towards these aims: (i) Developing fluorescence microscope based on Bessel light-sheet illumination to record by means calcium imaging. Chapter II describes...
Abstract Light-sheet microscopy (LSM) has proven a useful tool in neuroscience to image whole brains with high frame rates at cellular resolution. LSM is employed either combination tissue clearing reconstruct the cyto-architecture over entire mouse brain or intrinsically transparent samples like zebrafish larvae for functional imaging. Inherently LSM, however, residual opaque objects cause stripe artifacts, which obscure features of interest and, during imaging, modulate fluorescence...