A5072453787- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- 3D Printing in Biomedical Research
- Advanced Fluorescence Microscopy Techniques
- Proteoglycans and glycosaminoglycans research
- Cell Image Analysis Techniques
- Cell Adhesion Molecules Research
- Micro and Nano Robotics
- Polysaccharides and Plant Cell Walls
- Single-cell and spatial transcriptomics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Melanoma and MAPK Pathways
- Optical measurement and interference techniques
- Fibroblast Growth Factor Research
- Protein Kinase Regulation and GTPase Signaling
- Protein Degradation and Inhibitors
- Diffusion and Search Dynamics
- Microfluidic and Bio-sensing Technologies
- Studies on Chitinases and Chitosanases
- Congenital heart defects research
- Marine Invertebrate Physiology and Ecology
- Digital Image Processing Techniques
- Biosensors and Analytical Detection
Institute of Science and Technology Austria
2011-2023
Leiden University
2006-2014
Max Planck Institute of Molecular Cell Biology and Genetics
2010
Embryonic Cell Sorting and Movement Differential cell adhesion has long been thought to drive sorting. Maître et al. (p. 253 , published online 23 August) show that sorting in zebrafish gastrulation is triggered by differences the ability of cells modulate cortex tension at cell-cell contacts, thereby controlling contact expansion. functions this process mechanically coupling cortices adhering their allowing control In epiboly enveloping layer (EVL)—a surface epithelium formed animal pole...
Embryo morphogenesis relies on highly coordinated movements of different tissues. However, remarkably little is known about how tissues coordinate their to shape the embryo. In zebrafish embryogenesis, tissue first become apparent during "doming," when blastoderm begins spread over yolk sac, a process involving epithelial surface cell layer expansion and mesenchymal deep intercalations. Here, we find that active represents key coordinating doming. By using combination theory experiments,...
The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences adhesion and cortex tension are commonly thought to drive sorting by regulating tissue surface (TST). However, the role that differential TST plays within developing embryo as yet unclear. Here, we have analyzed for germ layer progenitor during zebrafish gastrulation. Contrary previous observations drives
Abstract Lymph nodes (LNs) comprise two main structural elements: fibroblastic reticular cells that form dedicated niches for immune cell interaction and capsular fibroblasts build a shell around the organ. Immunological challenge causes LNs to increase more than tenfold in size within few days. Here, we characterized biomechanics of LN swelling on cellular organ scale. We identified lymphocyte trapping by influx proliferation as drivers an outward pressure force, causing T-zone (TRCs) their...
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Tension of the actomyosin cell cortex plays a key role in determining cell-cell contact growth and size. The level cortical tension outside contact, when pulling at edge, scales with total size to which can grow [J.-L. Maître et al., Science 338, 253-256 (2012)]. Here, we show zebrafish primary germ-layer progenitor cells that this monotonic relationship only applies narrow range increase above critical threshold, inversely tension. This switch from increasing decreasing is caused by...
Abstract Translocations involving ETS ‐transcription factors, most commonly leading to the EWSR1–FLI1 fusion protein, are hallmark of Ewing sarcoma. Despite knowledge this driving molecular event, an effective therapeutic strategy is lacking. To test potential treatment regimes, we established a novel sarcoma zebrafish engraftment model allowing time‐effective, dynamic quantification progression and tumour burden in vivo , applicable for screening single combined compounds. In...
We demonstrate correlation analysis of the photothermal signal from gold nanoparticles diffusing in solution. Our experimental setup is primarily optimized for large detection volumes and therefore has a relatively low numerical aperture (0.25). This requirement limits light collection efficiency, but we can nevertheless detect particles as small 20 nm diameter 100 μs. Photothermal spectroscopy (PCS) water water:glycerol mixtures gives access to characteristic diffusion times some tens...
The MAPK signaling proteins are involved in many eukaryotic cellular processes and networks. However, specific functions of most these vertebrate development remain elusive because potential redundancies. For instance, the upstream activation pathways for ERK1 ERK2 highly similar, also their known downstream targets common. In contrast, mice zebrafish studies indicate distinct roles both ERKs proliferation, oncogenic transformation development. A major bottleneck further is that relatively...
Compared with the increasing use of zebrafish as a model organism in many laboratories, cell lines are still unexploited and limited application, partly due to their unknown genetic physiological properties. We characterize two embryonic fibroblast lines, ZF4 PAC2. demonstrate stability these achieved manipulation by either lipid-mediated transfection or an electroporation- based nucleofection method. Data from chip analysis (Affymetrix) unique characteristics gene expression levels, showing...
Tissue morphogenesis and patterning during development involve the segregation of cell types. Segregation is driven by differential tissue surface tensions generated types through controlling cell-cell contact formation regulating adhesion actomyosin contractility-based cellular cortical tensions. We use vertebrate zebrafish germ layer progenitors as in vitro models 3-dimensional heterotypic developed a quantitative analysis their dynamics based on 3D time-lapse microscopy. show that general...
Abstract Although the underlying molecular mechanism of hepatocellular carcinoma remains unclear, signalling pathways essential in cell survival and growth are altered, including Raf–MEK–MAPK pathway. This pathway can be activated by hepatitis B or C virus infections ectopic expression Raf‐1 oncogene is frequently seen carcinomas. In addition, was also shown to deregulated zebrafish liver tumours. Based on genetic conservation between human tumours, used as an animal model better understand...
The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification apoplast cell walls (Fendrych et al., 2016). Here, we describe in detail method for analysis elongation TIR1/AFB-Aux/IAA-dependent auxin response hypocotyl segments as well determination relative values wall pH.
Chitin oligosaccharides (COs) play a role in plant development and are presumed to affect body plan formation during vertebrate embryogenesis. The mechanisms of COs recognition cellular processes underlying embryonic still not understood. We analyze the possible link with mitogen-activated protein kinase pathway that is conserved evolution through animal kingdom has been implicated diverse processes, including cell growth, proliferation, differentiation, survival, development. show vivo...
We consider the simultaneous movement of finitely many colored points in space, calling it a spatial sorting process. The name suggests purpose that drives collection to configuration increased or decreased order. Mapping such process subset space-time, we use persistent homology measurements time function characterize topologically.
To obtain accurate, reproducible, and interpretable data when conducting imaging experiments, it is critical to consider external factors affecting acquisition at various steps of the experimental workflow. Illumination power stability represent two factors, especially comparing fluorescence intensities between images during a time-lapse experiment or experiments performed different times on microscopes. The signal can be generated by types light sources. These sources their coupling...