A5020031273- Axon Guidance and Neuronal Signaling
- Cellular Mechanics and Interactions
- Neurobiology and Insect Physiology Research
- Advanced Proteomics Techniques and Applications
- Developmental Biology and Gene Regulation
- Neurogenesis and neuroplasticity mechanisms
- Molecular Biology Techniques and Applications
- Cell Image Analysis Techniques
- Metabolomics and Mass Spectrometry Studies
- Ion channel regulation and function
- Signaling Pathways in Disease
- Mass Spectrometry Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Protein Kinase Regulation and GTPase Signaling
- Reproductive Biology and Fertility
- 3D Printing in Biomedical Research
- Cellular transport and secretion
- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- Prenatal Screening and Diagnostics
- Angiogenesis and VEGF in Cancer
- Lipid Membrane Structure and Behavior
- Cell Adhesion Molecules Research
Institute of Molecular Biotechnology
2020-2025
Austrian Academy of Sciences
2024-2025
Institute of Science and Technology Austria
2016-2022
Instituto de Neurociencias
2011-2013
Consejo Superior de Investigaciones Científicas
2012
ABSTRACT A detailed proteome map is crucial for understanding molecular pathways and protein functions. Despite significant advancements in sample preparation, instrumentation, data analysis, single-cell proteomics currently limited by proteomic depth quantitative performance. We combine a zero dead-end volume chromatographic column running at high throughput with the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer DIA mode. demonstrate unprecedented of coverage as well accuracy...
Tension of the actomyosin cell cortex plays a key role in determining cell-cell contact growth and size. The level cortical tension outside contact, when pulling at edge, scales with total size to which can grow [J.-L. Maître et al., Science 338, 253-256 (2012)]. Here, we show zebrafish primary germ-layer progenitor cells that this monotonic relationship only applies narrow range increase above critical threshold, inversely tension. This switch from increasing decreasing is caused by...
Axon guidance is a key process during nervous system development and regeneration. One of the best established paradigms to study mechanisms underlying this axon decision whether or not cross midline in <i>Drosophila</i> CNS. An essential regulator that well conserved Slit-Robo signaling pathway. Slit cues act through Robo receptors repel axons from midline. Despite good progress our knowledge about these proteins, intracellular associated with function remain poorly defined. In work, we...
It is firmly established that neuron-glia interactions are fundamental across species for the correct establishment of a functional brain. Here, we found glia Drosophila larval brain display an essential non-autonomous role during development optic lobe. The lobe develops from neuroepithelial cells proliferate by dividing symmetrically until they switch to asymmetric/differentiative divisions generating neuroblasts. proneural gene lethal scute (l'sc) transiently activated Epidermal Growth...
Vertebrates and insects alike use glial cells as intermediate targets to guide growing axons. Similar vertebrate oligodendrocytes, Drosophila midline glia ensheath separate axonal commissures. Neuron-glia interactions are crucial during these events, although the proteins involved remain largely unknown. Here, we show that Canoe (Cno), ortholog of AF-6, DE-cadherin Shotgun (Shg) highly restricted interface between commissural cno mutant analysis, genetic co-immunoprecipitation assays unveil...
Abstract Tension of the actomyosin cell cortex plays a key role in determining cell-cell contact growth and size. The level cortical tension outside contact, when pulling at edge, scales with total size to which can grow 1,2 . Here we show zebrafish primary germ layer progenitor cells that this monotonic relationship only applies narrow range increase, above critical threshold, inversely tension. This switch from increasing decreasing is caused by promoting E-cadherin anchoring cytoskeleton,...