A5052034831- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Cell Image Analysis Techniques
- Cancer, Lipids, and Metabolism
- RNA Research and Splicing
- Nuclear Receptors and Signaling
- Advanced Fluorescence Microscopy Techniques
- Cancer, Hypoxia, and Metabolism
- Cellular Mechanics and Interactions
- Inflammatory mediators and NSAID effects
- Protein Degradation and Inhibitors
- Genomics and Chromatin Dynamics
- Skin and Cellular Biology Research
- RNA modifications and cancer
- Estrogen and related hormone effects
- Advanced Electron Microscopy Techniques and Applications
- Calcium signaling and nucleotide metabolism
- Electron and X-Ray Spectroscopy Techniques
- Genomics and Phylogenetic Studies
- Lipid metabolism and biosynthesis
- Drug Transport and Resistance Mechanisms
- Cell Adhesion Molecules Research
- Cholesterol and Lipid Metabolism
- DNA Repair Mechanisms
Friedrich Miescher Institute
2007-2023
European Molecular Biology Organization
2023
University of Basel
2019
University of Lausanne
2004-2009
Novartis (Switzerland)
2008
Institut de Biologie Moléculaire et Cellulaire
2005
Institute of Bioinformatics
2005
Inserm
1999-2004
Centre National de la Recherche Scientifique
1993-2004
Institut de génétique et de biologie moléculaire et cellulaire
2002
Peroxisome proliferator-activated receptor γ (PPARγ) is a nuclear implicated in adipocyte differentiation and insulin sensitivity. We investigated whether PPARγ expression dependent on the activity of determination factor 1/sterol regulatory element binding protein 1 (ADD-1/SREBP-1), another transcription associated with both cholesterol homeostasis. Ectopic ADD-1/SREBP-1 3T3-L1 HepG2 cells induced endogenous mRNA levels. The related SREBP-2 likewise expression. In addition, depletion,...
In mammals, interactions between sequences within topologically associating domains enable control of gene expression across large genomic distances. Yet it is unknown how frequently such contacts occur, long they last and depend on the dynamics chromosome folding loop extrusion activity cohesin. By imaging chromosomal locations at high spatial temporal resolution in living cells, we show that are transient occur during course a cell cycle. Interactions become more frequent longer presence...
The nuclear peroxisome proliferator-activated receptor γ (PPARγ) activates the transcription of multiple genes involved in intra- and extracellular lipid metabolism. Several cofactors are crucial for stimulation or silencing transcriptional activities. two homologous p300 CREB-binding protein (CBP) have been shown to co-activate ligand-dependent activities several receptors as well ligand-independent activity androgen receptor. We show here that interaction between p300/CBP PPARγ is complex...
Fluorescence resonance energy transfer (FRET) allows the user to investigate interactions between fluorescent partners. One crucial issue when calculating sensitized emission FRET is correction for spectral bleed-throughs (SBTs), which requires calculate ratios intensities in and donor or acceptor settings, only are present. Theoretically, SBT should be constant. However, experimentally, these can vary as a function of fluorophore intensity, assuming constant values may hinder precise...
Abstract Optic atrophy 1 (OPA1) is a mitochondrial inner membrane protein that has an important role in fusion and structural integrity. Dysfunctional OPA1 mutations cause of the optic nerve leading to blindness. Here, we show innate immune system. Using conditional knockout mice lacking Opa1 neutrophils ( N∆ ), report lack reduces activity electron transport complex I neutrophils. This then causes decline adenosine-triphosphate (ATP) production through glycolysis due lowered NAD +...
The modulation of energetic homeostasis by pollutants has recently emerged as a potential contributor to the onset metabolic disorders. Diethylhexyl phthalate (DEHP) is widely used industrial plasticizer which humans are exposed. Phthalates can activate three peroxisome proliferator-activated receptor (PPAR) isotypes on cellular models and induce proliferation in rodents. In this study, we aimed evaluate systemic consequences DEHP exposure that have remained so far unexplored characterize...
Transcriptional activity relies on coregulators that modify chromatin structure or serve as bridging factors between transcription and the basal machinery. We identified a new coregulator of peroxisome proliferator-activated receptor γ, BRG1/Brm-associated factor 60 kDa, subunit c2 (BAF60c2), in yeast two-hybrid screen human adipose tissue cDNA library. BAF60c2 represents isoform BAF60c, component SWI/SNF (mating type switching/sucrose non-fermenting) remodeling complex. This well previously...
In a global approach combining fluorescence recovery after photobleaching (FRAP), correlation spectroscopy (FCS), and resonance energy transfer (FRET), we address the behavior in living cells of peroxisome proliferator-activated receptors (PPARs), family nuclear involved lipid glucose metabolism, inflammation control, wound healing. We first demonstrate that unlike several other receptors, PPARs do not form speckles upon ligand activation. The subnuclear structures may be observed under some...
3-Thia fatty acids are modified that promote hepatic peroxisome proliferation and decrease serum triacylglycerol, cholesterol free acid levels in rats. In vivo administration of tetradecylthioacetic (TTA) to rats led a significant liver apolipoproteins apoA-I, A-II, A-IV, C-III mRNA levels, an increase acyl-CoA oxidase (ACO), carnitine palmitoyltransferase-II, 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMG-CoA synthase) activities. By contrast, no changes lipoprotein lipase (LPL) were...
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Abstract Multiprotein bridging factor (MBF-1) is a cofactor that was first described for its capacity to modulate the activity of fushi tarazu 1, nuclear receptor originally implicated in Drosophila development. Recently, it has been shown human MBF-1 stimulates transcriptional steroidogenic homolog which steroidogenesis. Here we show this enhances several nonsteroid receptors are lipid metabolism, i.e. liver X α, and PPARγ. interacts with distinct domains these receptors, depending on...
Abstract Physical proximity between genomic sequences in mammalian chromosomes controls key biological processes such as transcriptional regulation and DNA repair. Yet it is currently unknown if chromosomal contacts are rare stable or instead frequent dynamic, how they depend on the loop extrusion activity of cohesin barriers CTCF. By imaging locations at high spatial temporal resolution over several hours living cells, we show that within topological associating domains (TADs) frequently...