A5006254248- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer, Stress, Anesthesia, and Immune Response
- Cell Image Analysis Techniques
- AI in cancer detection
- Pancreatic function and diabetes
- Radiomics and Machine Learning in Medical Imaging
- Cell Adhesion Molecules Research
- Diabetes and associated disorders
- RNA modifications and cancer
- CAR-T cell therapy research
- Single-cell and spatial transcriptomics
- ATP Synthase and ATPases Research
- Axon Guidance and Neuronal Signaling
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Genetics, Bioinformatics, and Biomedical Research
- Pancreatic and Hepatic Oncology Research
- Diabetes Management and Research
- Metabolism, Diabetes, and Cancer
- Radiopharmaceutical Chemistry and Applications
- Cellular Mechanics and Interactions
- Protein Tyrosine Phosphatases
- HER2/EGFR in Cancer Research
- Photoacoustic and Ultrasonic Imaging
City of Hope
2020-2025
University of Wisconsin–Madison
2022-2025
Beckman Research Institute
2019-2024
Henry M. Jackson Foundation
2024
City Of Hope National Medical Center
2020-2023
European Molecular Biology Organization
2023
BioNTech (United States)
2020
Regulus Therapeutics (United States)
2014
CD8+ tumor-infiltrating lymphocytes (TILs) correlate with relapse-free survival (RFS) in most cancer types, including breast cancer. However, subset composition, functional status, and spatial location of TILs relation to RFS human tumors remain unclear. Spatial tissue analysis via quantitative immunofluorescence showed that infiltration T cells into islands was more significantly associated than cell either tumor stroma or total tumor. Localization within is mediated by expression the...
CD8+ tumor-infiltrating lymphocytes (TILs) are associated with improved survival in triple-negative breast cancer (TNBC) yet have no association estrogen receptor-positive (ER+) BC. The basis for these contrasting findings remains elusive. We identified subsets of BC tumors infiltrated by T cells characteristic features exhausted (TEX). Tumors abundant TEX exhibited a distinct tumor microenvironment marked amplified interferon-γ signaling-related pathways and higher programmed death ligand 1...
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Tumor heterogeneity and cisplatin resistance are major causes of tumor relapse poor survival. Here, we show that in lung cancer, interaction between paxillin (PXN) integrin β4 (ITGB4), components the focal adhesion (FA) complex, contributes to resistance. Knocking down PXN ITGB4 attenuated cell growth improved sensitivity, both 2D 3D cultures. independently regulated expression several genes. In addition, they also common genes including USP1 VDAC1, which required for maintaining genomic...
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human cancers. However, symptoms and radiographic appearance chronic pancreatitis (CP) mimics that PDAC, sometimes 2 entities can also be difficult to differentiate microscopically. The need for accurate differentiation PDAC CP has become a major topic in pancreatic pathology. These diseases present similar histomorphological features, such as excessive deposition fibrotic stroma tissue microenvironment inflammatory cell...
Pancreatic islets are three-dimensional cell aggregates consisting of unique cellular composition, cell-to-cell contacts, and interactions with blood vessels. Cell aggregation is essential for islet endocrine function; however, it remains unclear how developing establish aggregation. By combining genetic animal models, imaging tools, gene expression profiling, we demonstrate that regulated by extracellular matrix signaling cell-cell adhesion. Islet cell-specific inactivation receptor...
Type 1 diabetes (T1D) is a progressive autoimmune condition that culminates in the loss of insulin-producing beta cells. Pancreatic histopathology provides essential insights into disease initiation and progression yet an integrated perspective situ pathogenic processes lacking due to limited sample availability, dispersed nature anatomical lesions, often restricted analytical dimensionality. Here, we combined multiplexed immunostaining, high-magnification whole-slide imaging, digital...
Abstract B-cell receptor (BCR) signaling plays an important role in the pathogenesis of mantle cell lymphoma (MCL), but detailed mechanisms are not fully understood. In this study, through a genome-wide loss-of-function screen, we identify carcinoembryonic antigen-related adhesion molecule 1 (CEACAM1) as essential factor subset MCL tumors. Our signal transduction studies reveal that CEACAM1 critical BCR activation involvement two dynamic processes. First, following engagement, co-localizes...
Abstract Aims/hypothesis Transcriptome analyses revealed insulin-gene-derived transcripts in non-beta endocrine islet cells. We studied alternative splicing of human INS mRNA pancreatic islets. Methods Alternative insulin pre-mRNA was determined by PCR analysis performed on RNA and single-cell RNA-seq analysis. Antisera were generated to detect variants tissue using immunohistochemistry, electron microscopy western blot confirm the expression variants. Cytotoxic T lymphocyte (CTL) activation...
Bioimage analysis (BIA), a crucial discipline in biological research, overcomes the limitations of subjective microscopy through creation and application quantitative reproducible methods. The establishment dedicated BIA support within academic institutions is vital to improving research quality efficiency can significantly advance scientific discovery. However, lack training resources, limited career paths insufficient recognition contributions made by bioimage analysts prevent full...
Abstract Protein phosphatase 2A (PP2A), a serine/threonine involved in the regulation of apoptosis, proliferation, and DNA-damage response, is overexpressed many cancers, including small cell lung cancer (SCLC). Here we report that LB100, molecule inhibitor PP2A, when combined with platinum-based chemotherapy, synergistically elicited an antitumor response both vitro vivo no apparent toxicity. Using inductively coupled plasma mass spectrometry, determined quantitatively sensitization via...
Heterogeneity of endothelial cell (EC) populations reflects their diverse functions in maintaining tissue's homeostasis. However, phenotypic, molecular, and functional properties are not entirely mapped. We use the Tie2-CreERT2;Rosa26-tdTomato reporter mouse to trace, profile, cultivate primary ECs from different organs. As paradigm platform, we this strategy study bone marrow cells (BMECs). Single-cell mRNA sequencing BMECs reveals that diversity native molecular signatures is transitorily...
Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging particular is currently undergoing rapid technological advancements. However for scientists wishing to publish the obtained images image analyses results, there date no unified guidelines. Consequently, microscopy data publications may be unclear or difficult interpret. Here we present community-developed checklists preparing light analysis publications. These offer authors, readers,...
The tyrosine kinase receptor ephrin A2 (EPHA2) is overexpressed in lung (LSCC) and head neck (HNSCC) squamous cell carcinomas. Although EPHA2 can inhibit tumorigenesis a ligand-dependent fashion via phosphorylation of Y588 Y772, it promote ligand-independent manner S897. Here, we show that Roundabout Guidance Receptor 1 (ROBO1) interact to form functional heterodimer. Furthermore, the ROBO1 ligand Slit Ligand 2 (SLIT2) ensartinib, an inhibitor EPHA2, attenuate growth HNSCC cells act...
The post‑translational modification of proteins by ubiquitinating enzymes plays a central role in number cellular functions, such as cell proteolysis, DNA repair, and signaling communication. Deubiquitinating (DUBs) disassemble ubiquitin chains remove moieties from proteins. Targeting DUBs cancer models has revealed an important for these tumorigenesis, they therefore have emerged attractive therapeutic targets. In the present study, effects three DUB inhibitors, PR‑619, RA‑9 LDN‑91946, on...
The androgen receptor (AR) plays a critical role in the development of prostate cancer (PCa) through activation androgen-induced cellular proliferation genes. Thus, blocking AR-mediated transcriptional is expected to inhibit growth and spread PCa. Using tailor-made splice-switching locked nucleic acid (LNA) oligonucleotides (SSOs), we successfully redirected splicing AR precursor (pre-)mRNA destabilized transcripts via introduction premature stop codons. Furthermore, SSOs simultaneously...
Abstract microRNAs, which control post-transcriptional gene expression by repressing mRNA translation and/or causing degradation, play important roles in human cancers. miR-221 is one of the two microRNAs miR-221/222 cluster located chromosome-X, targets key cancer pathways and inhibit several tumor suppressors including p27, p57, PTEN. up-regulated >2-fold 55% >5-fold 14% hepatocellular carcinoma (HCC) samples. Importantly, it has been shown preclinical HCC models that...
SUMMARY Pancreatic islets are 3-dimensional cell aggregates consisting of unique cellular composition, cell-to-cell contacts, and interactions with blood vessels. Cell aggregation is essential for islet endocrine function; however, it remains unclear how developing establish aggregation. By combining genetic animal models, imaging tools, gene expression profiling, we demonstrate that regulated by extracellular matrix signaling cell-cell adhesion. Islet cell-specific inactivation receptor...
Summary Tumor heterogeneity and cisplatin resistance are a major cause of tumor relapse poor survival. Here we show that in lung adenocarcinoma (LUAD), paxillin (PXN) integrin beta 4 (ITGB4) associated with progression, resistance. Silencing PXN ITGB4 render tolerant cells sensitive, immunologically neutralizing improves sensitivity. The N-terminal half is intrinsically disordered interacts to regulate expression USP1 VDAC1 which required for maintaining genomic stability mitochondrial...