A5018442027- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Angiogenesis and VEGF in Cancer
- Circular RNAs in diseases
- Lipid metabolism and disorders
- Atherosclerosis and Cardiovascular Diseases
- Industrial Technology and Control Systems
- Plant Virus Research Studies
- MicroRNA in disease regulation
- Insect symbiosis and bacterial influences
- Mitochondrial Function and Pathology
- Immune cells in cancer
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Industrial Automation and Control Systems
- Eicosanoids and Hypertension Pharmacology
- Renin-Angiotensin System Studies
- Cancer, Hypoxia, and Metabolism
- Nitric Oxide and Endothelin Effects
- Hedgehog Signaling Pathway Studies
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Radioactive contamination and transfer
City of Hope
2019-2024
Beijing Health Vocational College
2019-2024
Polytechnic University of Turin
2024
Beckman Research Institute
2019-2023
Sun Yat-sen University
2016
Third Affiliated Hospital of Sun Yat-sen University
2016
University of California, Riverside
2010-2011
Harbin Medical University
1991
In response to infection, invertebrates process replicating viral RNA genomes into siRNAs of discrete sizes guide virus clearance by interference. Here, we show that sequenced from fruit fly, mosquito, and nematode cells were all overlapping in sequence, suggesting a possibility using for genome assembly discovery. To test this idea, examined contigs assembled published small libraries discovered five previously undescribed viruses cultured Drosophila adult mosquitoes, including three with...
Abstract Chromatin-associated RNA (caRNA) has been proposed as a type of epigenomic modifier. Here, we test whether environmental stress can induce cellular dysfunction through modulating RNA-chromatin interactions. We endothelial cell (EC) with high glucose and TNFα (H + T), that mimic the common in diabetes mellitus. characterize H T-induced changes gene expression by single (sc)RNA-seq, DNA interactions Hi-C, iMARGI. T inter-chromosomal interactions, particularly among super enhancers. To...
Metabolic disorders such as obesity and diabetes mellitus can cause dysfunction of endothelial cells (ECs) vascular rarefaction in adipose tissues. However, the modulatory role ECs tissue function is not fully understood. Other than growth factor-vascular factor receptor-mediated angiogenic signaling, little known about EC-derived signals regulation. We previously identified Argonaute 1 (AGO1; a key component microRNA-induced silencing complex) crucial regulator hypoxia-induced angiogenesis....
Chromatin-associated RNAs (caRNAs) form a relatively poorly recognized layer of the epigenome. The caRNAs reported to date are transcribed from nuclear genome. Here, leveraging recently developed assay for detection and their genomic association, we report that mitochondrial (mtRNAs) attached genome constitute subset caRNA, thus termed mt-caRNA. In four human cell types analyzed, mt-caRNAs preferentially attach promoter regions. endothelial cells (ECs), level mt-caRNA-promoter attachment...
Replication of viral RNA genomes in fruit flies and mosquitoes induces the production virus-derived small interfering RNAs (siRNAs) to specifically reduce virus accumulation by interference (RNAi). However, it is unknown whether RNA-based antiviral immunity (RVI) sufficiently potent terminate infection adult insects as occurs cell culture. We show here that, contrast robust Flock house (FHV), with an FHV mutant (FHVΔB2) unable express its RNAi suppressor protein B2 was rapidly terminated...
Abstract Background Compared to proteins, glycans, and lipids, much less is known about RNAs on the cell surface. We develop a series of technologies test for any nuclear-encoded that are stably attached surface exposed extracellular space, hereafter called membrane-associated (maxRNAs). Results technique Surface-seq selectively sequence maxRNAs validate two identified by RNA fluorescence in situ hybridization. To cell-type specificity maxRNA, we use antisense oligos hybridize...
Impaired angiogenesis in diabetes is a key process contributing to ischemic diseases such as peripheral arterial disease. Epigenetic mechanisms, including those mediated by long noncoding RNAs (lncRNAs), are crucial links connecting and the related chronic tissue ischemia. Here we identify lncRNA that enhances endothelial nitric oxide synthase (eNOS) expression (LEENE) regulator of response. LEENE was decreased diabetic conditions cultured cells (ECs), mouse hind limb muscles, human...
ABSTRACT Background Type 2 diabetes (T2D) is associated with accelerated vascular complications like hypertension and atherosclerosis. “Phenotypic switching” of smooth muscle cells (SMC), a major driver these complications, enhanced in diabetes. Despite adequate glycemic control, SMC dysfunction can persist due to “metabolic memory” prior hyperglycemia. However, the mechanisms hyperglycemic memory persistent are unclear. Here, leveraging single-cell (sc) multi-omics, we examined effect...
BACKGROUND: Endothelial cell (EC) dysfunction is both a cause and consequence of vascular inflammation lipid dysregulation in atherosclerosis, yet the molecular drivers linking EC to systemic metabolic derangements remain incompletely understood. We previously identified Argonaute 1 (AGO1)—a canonical component RNA-induced silencing complex—as regulator function angiogenesis metabolism. In this study, we uncover unrecognized, non-canonical role nuclear AGO1 ECs as transcriptional coactivator...
Vasculopathies occur 15 years earlier in individuals with diabetes mellitus (DM) as compared to those without, but the underlying mechanisms driving diabetic vasculopathy remain incompletely understood. Endothelial cells (ECs) and macrophages (MΦ) are critical players vascular wall their crosstalk is crucial vasculopathy. In diabetes, EC activation enables monocyte recruitment, which transmigrate into intima differentiate (MΦ). Beyond this established model of diapedesis, EC-MΦ interplay...
Long non-coding RNAs (lncRNAs) have previously been emerged as key players in a series of biological processes. Dysregulation lncRNA is correlated to human diseases including neurological disorders. Here, we developed multi-step bioinformatics analysis study the functions particular Down syndrome-associated gene DSCR9 lncRNAs. The method named correlation-interaction-network (COIN), based on which pipeline implemented. Co-expression network and results are presented. We identified regulation...
The homeostasis of vascular endothelium is crucial for cardiovascular health and endothelial cell (EC) aging dysfunction could negatively impact function. Leveraging transcriptome profiles from ECs subjected to various stimuli, including time-series data obtained under physiological pulsatile flow vs. pathophysiological oscillatory flow, we performed principal component analysis (PCA) identify key genes contributing divergent transcriptional states ECs. Through bioinformatics analysis,...
Heterogeneity of endothelial cell (EC) populations reflects their diverse functions in maintaining tissue's homeostasis. However, phenotypic, molecular, and functional properties are not entirely mapped. We use the Tie2-CreERT2;Rosa26-tdTomato reporter mouse to trace, profile, cultivate primary ECs from different organs. As paradigm platform, we this strategy study bone marrow cells (BMECs). Single-cell mRNA sequencing BMECs reveals that diversity native molecular signatures is transitorily...
Vascular endothelial cells (ECs) play a pivotal role in whole body homeostasis. Recent advances have revealed enhancer-associated long non-coding RNAs (lncRNAs) as essential regulators EC function. We investigated LINC00607, super enhancer-derived lncRNA (SE-lncRNA) human arteries with an emphasis on ECs. Based public databases and our single cell RNA-sequencing (scRNA-seq) data from collected healthy diabetic donors, we found that LINC00607 is abundantly expressed the its level increased...
Endothelial cells (ECs) are crucial for vascular and whole-body function through their dynamic response to environmental cues. Elucidating the transcriptome epigenome of ECs is paramount understanding roles in development, health, disease, but limited availability isolated primary cells. Recent technologies have enabled high-throughput profiling EC epigenome, leading identification previously unknown cell subpopulations developmental trajectories. While cultures a useful tool exploration...
(1) Background: Hypertension is a complex, multifactorial disease that caused by genetic and environmental factors. Apart from predisposition, the mechanisms involved in this have yet to be fully understood. We previously reported LEENE (lncRNA enhancing endothelial nitric oxide expression, transcribed LINC00520 human genome) regulates cell (EC) function promoting expression of synthase (eNOS) vascular growth factor receptor 2 (VEGFR2). Mice with deletion LEENE/LINC00520 homologous region...
Cardiovascular disease (CVD) occurs 15 years earlier in diabetics compared to those without, but the underlying mechanisms driving diabetes-accelerated CVDs (DACVDs) pathogenesis remains incompletely understood. Endothelial cells (ECs) and macrophages (MΦ) are key players vascular wall their crosstalk is crucial DACVD. In diabetes, ECs activation enables monocytes recruitment, which transmigrate across intima differentiate into (MΦ). Beyond this established diapedesis model, EC-MΦ interplay...
Background: Dysfunctional endothelial cells (ECs) crucially contribute to atherosclerosis (AS) largely through vascular inflammation. Beyond this, understanding the role of EC dysfunction (ECD) in liver and hyperlipidemia, another AS driver, is unclear. Argonaute 1 (AGO1), a core protein RNA-induced silencing complex, was found regulate response hypoxic metabolic stresses our previous studies. conditional AGO1 knockout (EC-AGO1-KO) mice are protected from diet-induced obesity. However,...
Vascular endothelial cells (ECs) play a pivotal role in whole-body homeostasis. Recent advances have revealed enhancer-associated long non-coding RNAs (lncRNAs) as essential regulators EC function. We investigated LINC00607, super enhancer-derived lncRNA (SE-lncRNA) human arteries with an emphasis on ECs. Based public databases and our single-cell RNA-sequencing (scRNA-seq) data from collected healthy diabetic donors, we found that LINC00607 is abundantly expressed the its level increased...
Mitochondrial-nuclear communication is vital for cellular homeostasis and response to environmental stress. The known messengers mitochondria communicate their functional status the nucleus include ATP, ROS, Ca 2+ . It remains unclear whether immediate output of mitochondrial transcription, i.e., RNAs (mtRNAs), are a type messenger nucleus. Herein, we show that mt attached nuclear genome thus constitute subset c hromatin- ssociated RNA (mt-caRNA). Mt-caRNAs preferentially attach promoter...