A5100353435- HIV Research and Treatment
- Cardiovascular Function and Risk Factors
- Advanced biosensing and bioanalysis techniques
- Adipose Tissue and Metabolism
- Receptor Mechanisms and Signaling
- Cytomegalovirus and herpesvirus research
- Viral Infections and Vectors
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Atrial Fibrillation Management and Outcomes
- RNA Research and Splicing
- Cholesterol and Lipid Metabolism
- Reproductive System and Pregnancy
- interferon and immune responses
- Influenza Virus Research Studies
- Respiratory viral infections research
- Renin-Angiotensin System Studies
- Mosquito-borne diseases and control
- Cardiac Imaging and Diagnostics
- Animal Genetics and Reproduction
- Glaucoma and retinal disorders
- RNA Interference and Gene Delivery
- Blood Pressure and Hypertension Studies
- Nitric Oxide and Endothelin Effects
- Analytical Chemistry and Sensors
Chinese Academy of Sciences
2016-2025
University of California, San Diego
2008-2025
Central South University
2013-2025
China Medical University
2016-2025
First Hospital of China Medical University
2019-2025
Hunan University of Traditional Chinese Medicine
2024-2025
The First Affiliated Hospital, Sun Yat-sen University
2023-2025
Sun Yat-sen University
2023-2025
City of Hope
2023-2025
Beckman Research Institute
2023-2025
Drought stress induces anthocyanin biosynthesis in many plant species, but the underlying molecular mechanism remains unclear. Ethylene response factors (ERFs) play key roles growth and various responses, including affecting biosynthesis. Here, we characterized an ERF protein, MdERF38, which is involved drought stress-induced Biochemical analyses showed that MdERF38 interacted with MdMYB1, a positive modulator of biosynthesis, facilitated binding MdMYB1 to its target genes. Therefore,...
Bimetallic nanoclusters (NCs) with superior performance to that of monometallic have attracted extensive research interest due the synergetic effect two atoms. Inspired from silver on enhanced fluorescence intensity Au NCs, a series bovine serum albumin-protected Au–Ag bimetallic NCs were prepared by regulating molar ratios HAuCl4/AgNO3 and their electrochemiluminescence (ECL) property was investigated using triethylamine as co-reactant. Notably, multifold higher efficiency achieved in...
To provide the clinical and molecular characteristics of carbapenem-resistant hypervirulent (hypermucoviscous) Klebsiella pneumoniae (cr-hvKP) in a tertiary hospital Beijing, China.The four patients with cr-hvKP isolates 29 classic K. (cr-cKP) infections were analyzed retrospectively. The cr-cKP compared.The KPC-2 gene was detected all cr-hvKPs except for cr-hvKP6. belonged to three sequence types (STs; ST25, ST65, ST11), pulsed-field gel electrophoresis patterns (I, II, III) two capsular...
Human Enterovirus 71 (EV71) and Coxsackievirus B3 (CVB3) have high risks for morbidity mortality. A virus quantitation immunoassay has been proposed by employing two colored quantum dots (QDs), antibodies of the virus, graphene oxide (GO). The QDs are streptavidin-conjugated (SA-QDs), biotinylated antibodies. Biotinylated EV71 antibody (Ab1) was associated with 525 nm green SA-QDs via biotin-streptavidin interaction forming QDs-Ab1, whereas CVB3 (Ab2) 605 red QDs-Ab2. GO an excellent...
Herein, a multifunctional nanoarchitecture has been developed by integrating the branched poly(ethylenimine) functionalized graphene/iron oxide hybrids (BGNs/Fe3O4) and luminol capped gold nanoparticles (luminol-AuNPs). The luminescent luminol-AuNPs as an electrochemiluminescence marker can be assembled on nanocarrier of BGNs/Fe3O4 efficiently via Au–N chemical bonds electrostatic adsorption. Meanwhile, proved with excellent electron transfer, good stability, high emission intensity, etc....
In the present work, an enhanced and stable anodic electrochemiluminescence (ECL) was observed from a suspension of boron nitride quantum dots (BNQDs) Ru(bpy)32+, which had 400-fold enhancement compared with individual Ru(bpy)32+. Interestingly, different previous research on BNQDs as type optical probe, were demonstrated efficient coreactant Ru(bpy)32+-based ECL for first time confirmed by collecting spectra. The amino-bearing groups electrocatalytic effect endowed them potential...
In this work, a novel style of microfluidic-based bipolar system with two-direction driving electrodes and dual-channel configuration was described for the first time, which could reach 100% current efficiency in theory. More importantly, background signal from integrated completely eliminated, when unique design used to construct an electrochemiluminescence (ECL) sensing platform. First, universal pH indicator employed study mechanism demonstrate that new possessed theoretically. Then,...
Atherosclerosis is a leading cause of death globally. Targeted imaging and therapeutics are desirable for the detection treatment disease. In this study, we developed trifunctional Simian virus 40 (SV40)-based nanoparticles in vivo targeting atherosclerotic plaques. These novel SV40-based encapsulate near-infrared quantum dots bear element drug component. Using nanoparticles, were able to noninvasively fluorescently image plaques live intact ApoE(−/−) mice. Near-infrared encapsulated SV40...
In this work, we report a nanoscale multichannel closed bipolar electrode (BPE) array based on the poly(ethylene terephthalate) (PET) membrane for first time. With our design, oxidants, coreactants, quenchers, and even biomarkers can be detected in Ru(bpy)3(2+)/TPA (tripropylamine) electrochemiluminescence (ECL) system. The PET was etched according to desire by NaOH, then Au nanofibers were decorated inner region of channel as BPE array. Using ECL signal readout, series targets including...
Cognitive deficit is thought to represent, at least in part, genetic mechanisms of risk for schizophrenia, with recent evidence from statistical modelling twin data suggesting direct causality the former latter. However, earlier was based on inferences not molecular and it unclear how much influence 'passes through' cognition way diagnosis. Thus, we included measurements (e.g. schizophrenia polygenic scores) causation models assess extent which cognitive mediates some effect scores disorder....
Abstract Cross‐protective and non‐invasively administered vaccines are attractive highly desired for the control of influenza. Self‐assembling nanotechnology provides an opportunity development with superior performance. In this study, intranasal nanovaccine is developed targeting conserved ectodomain influenza matrix protein 2(M2e). 3‐sequential repeats M2e (3M2e) presented on self‐assembling recombinant human heavy chain ferritin (rHF) cage to form 3M2e‐rHF nanoparticle. Intranasal...
Significance Influenza A virus (IAV) is one of the most important human pathogens, and it crucial to understand its life cycle develop antiviral strategies. However, IAV uncoating, an essential step in viral infection, has remained incomprehensible. Here, via construction infectious virions encapsulating quantum dots, we tracked uncoating ribonucleoprotein complex (vRNP) dynamics single virions. Our results reveal that after fusion vRNP segments are released separately into cytosol,...
The development of a universal influenza vaccine to control public health threats from circulating and emerging viruses is highly desirable. Here we report an intranasal multivalent epitope-based nanoparticle with broad protection against divergent A B viruses. Three conserved epitopes consisting the α-helix hemagglutinin (H), ectodomain matrix protein 2 (M) HCA-2 neuraminidase (N) are presented on self-assembling recombinant human heavy chain ferritin cage (F) generate HMNF nanoparticle....
Abstract Despite continued advances in prevention and treatment strategies, cardiovascular diseases (CVDs) remain the leading cause of death worldwide, more effective therapeutic methods are urgently needed. Polygonatum is a traditional Chinese herbal medicine with variety pharmacological applications biological activities, such as antioxidant activity, anti-inflammation, antibacterial effect, immune-enhancing glucose regulation, lipid-lowering anti-atherosclerotic effects, diabetes...
In this work, we have developed a full-featured electrochemiluminescence (ECL) sensing platform based on multichannel closed bipolar system. Owing to the three-channel and double-bipolar electrode (BPE) configuration, all oxidants, reductants, or chemicals that are directly related ECL process can be detected in single device, which greatly expanded application range of Ru(bpy)3(2+)-TPA (tripropylamine) anodic reaction. First all, more universal accurate mechanism for systems was proposed by...
A novel avian influenza A(H7N9) virus causing human infection emerged in February 2013 China. To elucidate the mechanism of interspecies transmission, we compared signature amino acids viruses from and non-human hosts analysed reassortants 146 with full genome sequences. We propose a genetic tuning procedure continuous acid substitutions reassorting that mediates host adaptation transmission. When early virus, containing ancestor haemagglutinin (HA) neuraminidase (NA) genes similar to...
Abstract The cytosolic DNA sensor cyclic GMP-AMP synthase (cGAS) mediates innate immune responses against invading pathogens, or self-dsDNA, which causes autoimmune disorders. Upon nonspecific binding of B–form DNA, cGAS synthesizes the second messenger 2′3′-cGAMP and triggers STING-dependent signaling to produce type I IFNs. comprises less-conserved N-terminal residues highly conserved nucleotidyltransferase/Mab21 domains. function structure well-conserved domains have been extensively...
Macrophages are one of the major targets human immunodeficiency virus (HIV-1), but viral entry pathway remains poorly understood in these cells. Noninvasive labeling and single-virus tracking effective tools for studying entry. Here, we constructed a quantum dot (QD)-encapsulated infectious HIV-1 particle to track at single-particle level live primary macrophages. QDs were encapsulated virions by incorporating accessory protein Vpr-conjugated during assembly. With particles encapsulating...
Background There are several mechanisms, including inflammation, oxidative stress and abnormal calcium homeostasis, involved in the pathogenesis of atrial fibrillation. In diabetes mellitus ( DM ), increased may be attributable to higher xanthine oxidase activity. this study, we examined relationship between electrical structural remodeling, handling abnormalities, potential beneficial effects inhibitor allopurinol upon these pathological changes. Methods Results Ninety rabbits were randomly...