A5083350636- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Quantum Dots Synthesis And Properties
- Nanocluster Synthesis and Applications
- Carbon and Quantum Dots Applications
- Advanced Biosensing Techniques and Applications
- Molecular Junctions and Nanostructures
- Advanced Nanomaterials in Catalysis
- Molecular Sensors and Ion Detection
- Luminescence and Fluorescent Materials
- RNA Interference and Gene Delivery
- Electrochemical Analysis and Applications
- Bacteriophages and microbial interactions
- Gold and Silver Nanoparticles Synthesis and Applications
- Plant Virus Research Studies
- Cytomegalovirus and herpesvirus research
- Viral Infections and Immunology Research
- Crystallization and Solubility Studies
- Electrochemical sensors and biosensors
- Crystal structures of chemical compounds
- X-ray Diffraction in Crystallography
- Hepatitis B Virus Studies
- Infant Nutrition and Health
- Plant and Fungal Interactions Research
- Identification and Quantification in Food
Lingnan Normal University
2016-2025
Yunnan Investment Group (China)
2024-2025
Yunnan Agricultural University
2024
Citrus Research Institute
2024
Southwest University
2024
Wuhan University
2010-2015
Ministry of Education of the People's Republic of China
2011
Huadong Hospital
2011
In this communication, we demonstrate that graphene oxide (GO) greatly inhibits the peroxidatic activity of a horseradish peroxidase-mimicking DNAzyme. Combining observation with unique DNA/GO interactions, an ultrasensitive GO-based chemiluminescence DNA biosensing platform is developed.
Human Enterovirus 71 (EV71) and Coxsackievirus B3 (CVB3) have high risks for morbidity mortality. A virus quantitation immunoassay has been proposed by employing two colored quantum dots (QDs), antibodies of the virus, graphene oxide (GO). The QDs are streptavidin-conjugated (SA-QDs), biotinylated antibodies. Biotinylated EV71 antibody (Ab1) was associated with 525 nm green SA-QDs via biotin-streptavidin interaction forming QDs-Ab1, whereas CVB3 (Ab2) 605 red QDs-Ab2. GO an excellent...
High quality and facile DNA functionalized quantum dots (QDs) as efficient fluorescence nanomaterials are of great significance for bioimaging both in vitro vivo applications. Herein, we offer a strategy to synthesize DNA-functionalized Zn2+ doped CdTe QDs (DNA-QDs) through one-pot hydrothermal route. is directly attached the surface QDs. The as-prepared exhibit small size (3.85 ± 0.53 nm), high yield (up 80.5%), excellent photostability. In addition, toxicity has dropped considerably...
We report a new sensor combined two dimensional metal–organic framework (MOF), N,N-bis(2-hydroxy-ethyl)dithiooxamidato copper(II) (H2dtoaCu), with the hairpin-structured oligonucleotides and demonstrate its feasibility in detecting multiplexed sequence-specific DNA. The key component of this (MOF–MBs) is fluorescent oligonucleotide that allows MOFs to function as both "nanoscaffold" for "nanoquencher" fluorophore. An sequence fragment wild-type HBV (T1) reverse-transcription RNA HIV (T2)...
A new strategy for quantitatively detecting micrococcal nuclease (MNase) is proposed using electrostatic interaction-based fluorescence resonance energy transfer (FRET) between positively charged QDs and negatively dye-labeled single-stranded DNA (dye-ssDNA). Herein, we have made our attempt to develop a where the variation of FRET efficiency due change interaction ssDNA that result from cleavage dye-ssDNA by single-strand-specific nuclease. To demonstrate feasibility this design, (lysozyme...
Aflatoxin B1 (AFB1) is one of the most frequently-found mycotoxins in contaminated food. As content particularly low food, development probes to detect AFB1 foods with high sensitivity and selectivity an urgent social need for evaluation food quality. Numerous techniques have been developed monitor AFB1. Nevertheless, them require cumbersome, labor-consuming, sophisticated instruments, which limited their application. An aptamer a single, short nucleic acid sequence that capable recognizing...
A highly sensitive and selective DNA biosensor based on hybridization chain reaction is described, which combines CdTe quantum dots (QDs) a ruthenium complex. Based the variation of fluorescence signals QDs, target determined.
A simple method for preparing DNA-stabilized Ag nanoclusters (NCs) nanowires is presented. To fabricate the NCs nanowires, we use just two unmodified component strands and a long enzymatically produced scaffold. These form at room temperature have periodic sequence units that are available fluorescence assembled which formed three-way junction (TWJ) structure. can be clearly visualized by confocal microscopy. Furthermore, due to high efficiency of rolling circle amplification reaction in...
A simple, rapid colorimetry for DNA detection based on non-aggregated gold nanoparticles and magnetic beads has been developed with high selectivity sensitivity.
We have developed a new fluorescent immune ensemble probe comprised of conjugated lower toxic water-soluble CdTe:Zn2+ quantum dots (QDs) and Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2- antibody complex (Ru-Ab) for the dual-color determination human enterovirus 71 (EV71) in homogeneous solution. EV71 monoantibody was easily covalently with Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2 to form stable Ru-Ab, which acted both as an effective quencher QDs fluorescence capture virus antigen EV71. Herein, target can...
The pentapeptide Cys-Ala-Leu-Asn-Asn (CALNN) has been proved to be a powerful tool stabilize the AuNPs. These CALNN-capped AuNPs have used develop various bioanalysis platforms. In this paper, are robust for aggregation-based colorimetric immunoassays as well. A immunoassay strategy based upon antibody-induced assembly of functionalized Abscisic Acid glucose ester (ABA-GE) determination developed. ABA-functionalized aggregate in presence specific antibody, accompanied by color change...
Terminal protection of small molecule linked DNA assays amplified by Exo III-aided recycling is developed for the detection streptavidin–biotin interaction, with a limit 0.8 fM streptavidin in "turn-on" state, indicating that proposed method an ultrasensitive platform molecule–protein interactions.
Paris yunnanensis, also named as Rhizoma Paridis in the Chinese Pharmacopeia, is a perennial medicinal herb commonly grown Southwest China. However, several viruses have been found infecting this plant recent years. Using high-throughput sequencing (HTS) and Sanger sequencing, study obtained complete genome sequences of three capillovirus isolates one potyvirus isolate. Genomic phylogenetic analyses revealed that these are same virus newly reported capillovirus, polyphylla chlorotic mottle...
A new molecular beacon (MB) driven by two-photon excitation (TPE) using quantum dots as energy donor is constructed, which provides reduced direct of acceptor and free interferences from autofluorescence or scattering light in a complicated biological matrix.