A5051319899- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Esophageal and GI Pathology
- Tracheal and airway disorders
- Gallbladder and Bile Duct Disorders
- Foreign Body Medical Cases
- Protease and Inhibitor Mechanisms
- Advanced Optical Sensing Technologies
- Computational Drug Discovery Methods
- Neurotransmitter Receptor Influence on Behavior
- Alcoholism and Thiamine Deficiency
- Pediatric Hepatobiliary Diseases and Treatments
- Congenital Diaphragmatic Hernia Studies
- Biochemical Acid Research Studies
- Enzyme Catalysis and Immobilization
- Complement system in diseases
- Metabolism and Genetic Disorders
- Blood Coagulation and Thrombosis Mechanisms
- Cannabis and Cannabinoid Research
- Eosinophilic Esophagitis
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Eosinophilic Disorders and Syndromes
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatitis Pathology and Treatment
Indiana University Bloomington
2022-2025
Hungarian Academy of Sciences
2005-2021
HUN-REN Institute of Experimental Medicine
2012-2021
Borsod-Abaúj-Zemplén Megyei Kórház és Egyetemi Oktató Kórház
2020
Institute of Molecular Life Sciences
2004-2018
HUN-REN Research Centre for Natural Sciences
2018
Czech Academy of Sciences, Institute of Experimental Medicine
2011
Nathan Kline Institute for Psychiatric Research
2007
HUN-REN Szegedi Biológiai Kutatóközpont
2004
Semmelweis University
1973-1996
Persistent CB<sub>1</sub> cannabinoid receptor activity limits neurotransmitter release at various synapses throughout the brain. However, it is not fully understood how constitutively active receptors, tonic endocannabinoid signaling, and its regulation by multiple serine hydrolases contribute to synapse-specific calibration of probability. To address this question perisomatic dendritic GABAergic in mouse hippocampus, we used a combination paired whole-cell patch-clamp recording, liquid...
While our understanding of the nanoscale architecture anterograde synaptic transmission is rapidly expanding, qualitative and quantitative molecular principles underlying distinct mechanisms retrograde communication remain elusive. We show that a particular form tonic cannabinoid signaling essential for setting target cell-dependent variability. It does not require activity two major endocannabinoid-producing enzymes. Instead, by developing workflow physiological, anatomical, measurements at...
Few reports have described in detail a true autoactivation process, where no extrinsic cleavage factors are required to initiate the of zymogen. Herein, we provide structural and mechanistic insight into multidomain serine protease: mannose-binding lectin-associated protease-2 (MASP-2), first enzymatic component lectin pathway complement activation. We characterized proenzyme form MASP-2 catalytic fragment encompassing its C-terminal three domains solved crystal structure at 2.4 A...
Abstract Immunolabeling and autoradiography have traditionally been applied as the methods-of-choice to visualize collect molecular information about physiological pathological processes. Here, we introduce PharmacoSTORM super-resolution imaging that combines complementary advantages of these approaches enables cell-type- compartment-specific nanoscale measurements. We exploited rational chemical design for fluorophore-tagged high-affinity receptor ligands an enzyme inhibitor; demonstrated...
Placental Protein 13 (PP13), an early biomarker of preeclampsia, is a placenta-specific galectin that binds beta-galactosides, building-blocks ABO blood-group antigens, possibly affecting its bioavailability in blood.We studied PP13-binding to erythrocytes, maternal effect on serum PP13 and performance as predictor preeclampsia intrauterine growth restriction (IUGR). Datasets Caucasian (n = 1078) Hispanic 242) women were analyzed according blood groups. In vivo, vitro silico antigens...
Hypothalamic neurosecretory systems are fundamental regulatory circuits influenced by thyroid hormone. Monocarboxylate-transporter-8 (MCT8)-mediated uptake of hormone followed type 3 deiodinase (D3)-catalyzed inactivation represent limiting factors neuronal T3 availability. In the present study we addressed localization and subcellular distribution D3 MCT8 in neurons function their axons. Intense D3-immunoreactivity was observed axon varicosities external zone rat median eminence neurohaemal...
Pathological alterations of the ductal system found during ERCP in 1082 examinations were due to pancreatic carcinoma 54 patients. The diagnosis was confirmed by laparotomy or autopsy. Predominant findings 42 patients stenoses, prestenotic dilatation, caliber variation, stop contrast medium and displacement pancreatography. In 28 cholangiograms stenoses most common which localized one third cases proximal bile duct. Four types may be differentiated: a stenosing type, an obstructed tapering...
Bacteria use surface appendages called type IV pili to perform diverse activities including DNA uptake, twitching motility, and attachment surfaces. The dynamic extension retraction of are often required for these activities, but the stimuli that regulate dynamics remain poorly characterized. To address this question, we study bacterial pathogen Vibrio cholerae, which uses mannose-sensitive hemagglutinin (MSHA) attach surfaces in aquatic environments as first step biofilm formation. Here, a...
3-Phosphoglycerate kinase (PGK) is a two-domain hinge-bending enzyme. It still unclear how the geometry of active site formed during domain closure and catalytic residues are brought into optimal position for reaction. Comparison three-dimensional structures in various open closed conformations suggests large (10 Å) movement Lys 215 closure. This change would be required direct participation this side chain both catalyzed phospho transfer special anion-caused activation. To test multiple...
The objective of our present study is to develop novel inhibitors for MMP-2 acute cardioprotection. In a series pilot studies, substituted carboxylic acid derivatives were synthesized based on imidazole and thiazole scaffolds then tested in screeening cascade MMP inhibition. We found that the MMP-inhibiting effects acid-based compounds are superior efficacy comparison conventional hydroxamic same molecules. Based these results, 568-membered focused library was generated silico members docked...
Depending on the prevailing environmental conditions, neutrophilic granulocytes release extracellular vesicles (EV) which have either anti-inflammatory effects other neutrophils or pro-inflammatory and antibacterial effects. In present study we investigated molecular mechanisms underlying biogenesis of functionally heterogenic EVs. We show that selective stimulation Mac-1 integrin (complement receptor 3) by specific ligands initiates generation EVs are able to impair bacterial growth induce...