A5000985085- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- DNA Repair Mechanisms
- Genomics and Phylogenetic Studies
- Antimicrobial Peptides and Activities
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
Indiana University Bloomington
2019-2022
Bacteria use surface appendages called type IV pili to perform diverse activities including DNA uptake, twitching motility, and attachment surfaces. The dynamic extension retraction of are often required for these activities, but the stimuli that regulate dynamics remain poorly characterized. To address this question, we study bacterial pathogen Vibrio cholerae, which uses mannose-sensitive hemagglutinin (MSHA) attach surfaces in aquatic environments as first step biofilm formation. Here, a...
In bacteria, a condensed structure of FtsZ (Z-ring) recruits cell division machinery at the midcell, and Z-ring formation is discouraged over chromosome by poorly understood phenomenon called nucleoid occlusion. B. subtilis , occlusion has been reported to be mediated, least in part, DNA-membrane bridging protein, Noc.
A microfluidic system coupled with fluorescence microscopy is a powerful approach for quantitative analysis of bacterial growth. Here, we measure parameters growth and dynamic localization the cell division initiation protein FtsZ in Bacillus subtilis Consistent previous reports, found that after division, rings remain at poles, polar ring disassembly coincides rapid Z-ring accumulation midcell. In cells mutated minD, however, persist indefinitely, suggesting primary function Min...
During growth, bacteria increase in size and divide. Division is initiated by the formation of Z-ring, a ring-like cytoskeletal structure formed treadmilling protofilaments tubulin homolog FtsZ. FtsZ localization thought to be controlled Min Noc systems, here we explore why cell division fails at high temperature when systems are simultaneously mutated. Microfluidic analysis minD noc double mutant indicated that proto-Z-rings periodic interchromosome locations but rings failed mature become...
ABSTRACT A microfluidic system coupled with fluorescence microscopy is a powerful approach for quantitative analysis of bacterial growth. Here, we measure parameters growth and dynamic localization the cell division initiation protein FtsZ in Bacillus subtilis . Consistent previous reports, find that after rings remain at pole, ring disassembly coincides rapid Z-ring accumulation midcell. In cells mutated minD , however, polar persist indefinitely, suggesting primary function Min...
ABSTRACT Bacteria that divide by binary fission form FtsZ rings at the geometric midpoint of cell between bulk replicated nucleoids. In B. subtilis , DNA- and membrane-binding Noc protein is thought to mediate nucleoid occlusion prevent from forming over chromosome. To explore role Noc, we used time-lapse fluorescence microscopy monitor cells growing in microfluidic channels. Our data show does not formation chromosome or control division site selection. Instead, inhibits migration...
ABSTRACT Bacteria use surface appendages called type IV pili to perform diverse activities including DNA uptake, twitching motility, and attachment surfaces. Dynamic extension retraction of is often required for these activities, but the stimuli that regulate dynamics remain poorly characterized. To study this question, we bacterial pathogen Vibrio cholerae , which uses mannose-sensitive hemagglutinin (MSHA) attach surfaces in aquatic environments as first step biofilm formation. Here, find...
ABSTRACT During growth, bacteria increase in size and divide. Division is initiated by the formation of Z-ring, an intense ring-like cytoskeletal structure formed treadmilling protofilaments tubulin homolog FtsZ. FtsZ localization thought to be controlled Min Noc systems, here, we explore why cell division fails at high temperature when systems are simultaneously mutated. Microfluidic analysis a minD noc double mutant indicated that proto-Z-rings periodic inter-chromosome locations but rings...