A5051848813- Developmental Biology and Gene Regulation
- Cellular Mechanics and Interactions
- Marine Invertebrate Physiology and Ecology
- Planarian Biology and Electrostimulation
- Hippo pathway signaling and YAP/TAZ
- Congenital heart defects research
- Wnt/β-catenin signaling in development and cancer
- Fibroblast Growth Factor Research
- Neurogenesis and neuroplasticity mechanisms
- Micro and Nano Robotics
- Single-cell and spatial transcriptomics
- 3D Printing in Biomedical Research
- Ichthyology and Marine Biology
- Paleontology and Evolutionary Biology
- Evolution and Paleontology Studies
- Cell Image Analysis Techniques
- Morphological variations and asymmetry
- Advanced Fluorescence Microscopy Techniques
- Laser Applications in Dentistry and Medicine
- Genetics, Aging, and Longevity in Model Organisms
- Marine Ecology and Invasive Species
- Microtubule and mitosis dynamics
- Animal Genetics and Reproduction
- TGF-β signaling in diseases
- Historical and Linguistic Studies
University of Konstanz
2022-2024
Friedrich Miescher Laboratory
2019-2023
Max Planck Society
2019-2023
Institute of Science and Technology Austria
2013-2019
University of Vienna
2013
During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools temporally controlling within embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how affects fate specification during zebrafish gastrulation. By using this manipulate duration vivo by light, show that extended organizer promotes prechordal plate suppresses endoderm differentiation. Endoderm differentiation...
Abstract Evolutionarily conserved signaling pathways are essential for early embryogenesis, and reducing or abolishing their activity leads to characteristic developmental defects. Classification of phenotypic defects can identify the underlying mechanisms, but this requires expert knowledge classification schemes have not been standardized. Here we use a machine learning approach automated phenotyping train deep convolutional neural network, EmbryoNet, accurately zebrafish mutants in an...
The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences adhesion and cortex tension are commonly thought to drive sorting by regulating tissue surface (TST). However, the role that differential TST plays within developing embryo as yet unclear. Here, we have analyzed for germ layer progenitor during zebrafish gastrulation. Contrary previous observations drives
Abstract The hindered diffusion model postulates that the movement of a signaling molecule through an embryo is affected by tissue geometry and binding-mediated hindrance, but these effects have not been directly demonstrated in vivo. Here, we visualize extracellular binding individual molecules activator-inhibitor pair Nodal Lefty live developing zebrafish embryos using reflected light-sheet microscopy. We observe coefficients are high cavities, whereas mobility reduced bound fractions...
BMP signaling has a conserved function in patterning the dorsal-ventral body axis Bilateria and directive anthozoan cnidarians. So far, cnidarian studies have focused on role of different network components regulating pSMAD1/5 gradient formation. Much less is known about target genes downstream signaling. To address this, we generated genome-wide list direct Nematostella vectensis , several which were Drosophila Xenopus . Our ChIP-seq analysis revealed that many regulatory molecules with...
Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives epithelial tissues, it remains unclear whether such instructive activity is also critical mesenchymal migration. Here, we developed light-activated version the receptor Frizzled 7 (Fz7) to analyze how affects anterior axial mesendoderm (prechordal plate, ppl) within zebrafish...
Abstract During animal development, embryos undergo complex morphological changes over time. Differences in developmental tempo between species are emerging as principal drivers of evolutionary novelty, but accurate description these processes is very challenging. To address this challenge, we present here an automated and unbiased deep learning approach to analyze the similarity different timepoints. Calculation similarities across stages resulted phenotypic fingerprints, which carry...
ABSTRACT Avian forelimb digit homology remains one of the standard themes in comparative biology and EvoDevo research. In order to resolve apparent contradictions between embryological paleontological evidence a variety hypotheses have been presented recent years. The proposals range from excluding birds dinosaur clade, assignments by different criteria, or even assuming hexadactyl tetrapod limb ground state. At present two approaches prevail: frame shift hypothesis pyramid reduction...
During vertebrate embryogenesis, the germ layers are patterned by secreted Nodal signals. In classical model, Nodals elicit signaling binding to a complex comprising Type I/II Activin receptors (Acvr) and co-receptor Tdgf1. However, it is currently unclear whether receptor can also affect distribution of themselves through embryo, unknown which putative Acvr paralogs mediate in zebrafish. Here, we characterize three I (Acvr1) four II (Acvr2) homologs show that – except for Acvr1c all...
Abstract The central question in developmental biology is how an embryo self-organizes from a ball of cells into structured animal. We use experimental and theoretical approaches to study the activity small number signaling molecules spatiotemporally controlled allow for embryonic self-construction. Here, we propose machine learning could be harnessed gain new understanding mechanisms by which interactions between pathways control robust embryogenesis morphology changes evolution.