A5019917058- Marine Ecology and Invasive Species
- Zebrafish Biomedical Research Applications
- Marine Invertebrate Physiology and Ecology
- Developmental Biology and Gene Regulation
- Advanced Fluorescence Microscopy Techniques
- Congenital heart defects research
- Neurogenesis and neuroplasticity mechanisms
- Protist diversity and phylogeny
- Molecular Biology Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Toxic Organic Pollutants Impact
- Planarian Biology and Electrostimulation
- TGF-β signaling in diseases
- RNA Interference and Gene Delivery
- Transgenic Plants and Applications
- Marine and coastal plant biology
- MicroRNA in disease regulation
- Animal Genetics and Reproduction
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
University of Vienna
2022-2025
Friedrich Miescher Laboratory
2018-2022
Max Planck Society
2018-2022
Abstract The hindered diffusion model postulates that the movement of a signaling molecule through an embryo is affected by tissue geometry and binding-mediated hindrance, but these effects have not been directly demonstrated in vivo. Here, we visualize extracellular binding individual molecules activator-inhibitor pair Nodal Lefty live developing zebrafish embryos using reflected light-sheet microscopy. We observe coefficients are high cavities, whereas mobility reduced bound fractions...
Abstract Endomesoderm specification by a maternal β-catenin signal and body axis patterning interpreting gradient of zygotic Wnt/β-catenin signalling was suggested to predate the split between Bilateria their sister clade Cnidaria. However, in Cnidaria, roles these processes have not been demonstrated directly. Here, tagging endogenous cnidarian Nematostella vectensis , we confirm that its oral-aboral is indeed patterned signalling. Strikingly, show that, contrast bilaterians, endomesoderm...
Abstract Fluorescence Recovery After Photobleaching (FRAP) and inverse FRAP (iFRAP) assays can be used to assess the mobility of fluorescent molecules. These measure diffusion by monitoring return fluorescence in bleached regions (FRAP), or dissipation from photoconverted (iFRAP). However, current FRAP/iFRAP analysis methods suffer simplified assumptions about sample geometry, bleaching/photoconversion inhomogeneities, underlying reaction-diffusion kinetics. To address these shortcomings, we...
Abstract BMP signaling patterns secondary body axes throughout Bilateria and, strikingly, in the bilaterally symmetric corals and sea anemones – members of bilaterian sister clade Cnidaria. It has been suggested that secondary, “directive” axis anemone Nematostella vectensis requires Chordin-mediated “shuttling” ligands, like Drosophila or frog, however, an alternative “local inhibition” model is also possible. To choose between these two options, we generated localized Chordin sources...
During vertebrate embryogenesis, the germ layers are patterned by secreted Nodal signals. In classical model, Nodals elicit signaling binding to a complex comprising Type I/II Activin receptors (Acvr) and co-receptor Tdgf1. However, it is currently unclear whether receptor can also affect distribution of themselves through embryo, unknown which putative Acvr paralogs mediate in zebrafish. Here, we characterize three I (Acvr1) four II (Acvr2) homologs show that – except for Acvr1c all...
Diffusion is essential for biochemical processes because it dominates molecular movement on small scales. Enzymatic reactions, example, require fast exchange of substrate and product molecules in the local environment enzyme to ensure efficient turnover. On larger spatial scales, diffusion secreted signaling proteins thought limit extent tissue differentiation during embryonic development. While possible measure vivo, specifically interfering with testing models directly remains challenging....
Abstract Endomesoderm specification based on a maternal β-catenin signal and axial patterning by interpreting gradient of zygotic Wnt/β-catenin signalling was suggested to predate the split between Bilateria their evolutionary sister Cnidaria. However, in Cnidaria, roles both these processes have not been proven directly. Here, tagging endogenous protein sea anemone Nematostella vectensis , we show that oral-aboral axis cnidarian is indeed patterned signalling. Unexpectedly, striking...
Abstract The influential hindered diffusion model postulates that the global movement of a signaling molecule through an embryo is affected by local tissue geometry and binding-mediated hindrance, but these effects have not been directly demonstrated in vivo for any molecule. Nodal Lefty are prime example activator-inhibitor pair whose different diffusivities thought to arise from differential hindrance. Here, we used single-molecule tracking probe tenets on nanoscale. We visualized...