A5057573640- Retinal Development and Disorders
- Marine Ecology and Invasive Species
- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Planarian Biology and Electrostimulation
- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- Marine Invertebrate Physiology and Ecology
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Congenital heart defects research
- Plant and Biological Electrophysiology Studies
- RNA modifications and cancer
- Reproductive biology and impacts on aquatic species
- Psychology Research and Bibliometrics
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- TGF-β signaling in diseases
- MicroRNA in disease regulation
- E-Learning and Knowledge Management
- Corneal Surgery and Treatments
- Health and Medical Education
Centro Andaluz de Biología del Desarrollo
2018-2024
Friedrich Miescher Laboratory
2018-2022
Max Planck Society
2018-2022
Universidad Pablo de Olavide
2018-2021
Consejo Superior de Investigaciones Científicas
2021
Universitat de Barcelona
2011-2018
Institute for Research in Biomedicine
2011-2014
Regeneration of lost tissues depends on the precise interpretation molecular signals that control and coordinate onset proliferation, cellular differentiation cell death. However, nature those mechanisms integrate responses remain largely unknown. The planarian flatworm is a unique model in which regeneration tissue renewal can be comprehensively studied vivo. presence population adult pluripotent stem cells combined with ability to decode signaling after wounding enable planarians...
The Wingless/Integrated (Wnt) signaling pathway controls multiple events during development and homeostasis. It comprises branches, mainly classified according to their dependence on β-catenin activation. Wnt/β-catenin branch is essential for the establishment of embryonic anteroposterior (AP) body axis throughout phylogenetic tree. also required AP planarian regeneration. Wnt/β-catenin–independent encompasses several different pathways, which most extensively studied planar cell polarity...
Abstract Coordinated chromatin interactions between enhancers and promoters are critical for gene regulation. The architectural protein CTCF mediates looping is enriched at the boundaries of topologically associating domains (TADs), which sub-megabase structures. In vitro depletion leads to a loss TADs but has only limited effects over expression, challenging concept that CTCF-mediated structures fundamental requirement However, how perturbed structure impacts expression during development...
Spermatogenesis is the biological process by which male sperm cells (spermatozoa) are produced in testes. Beyond facilitating transmission of genetic information, spermatogenesis also provides a potential framework for inter- and transgenerational inheritance gene-regulatory states. While extensively studied mammals, our understanding anamniotes remains limited. Here we present comprehensive single-cell multiomics resource, combining RNA sequencing (scRNA-seq) chromatin accessibility...
Genetic studies in human and mice have established a dual role for Vsx genes retina development: an early function progenitors’ specification, later requirement bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent functions are also across vertebrates, as mutant models available only mammals. To gain insight into vsx teleosts, we generated vsx1 vsx2 CRISPR/Cas9 double knockouts ( KO) zebrafish. Our electrophysiological...
Abstract The assembly of the embryo’s primary axis is a fundamental landmark for establishment vertebrate body plan. Although morphogenetic movements directing cell convergence towards midline have been described extensively, little known on how gastrulating cells interpret mechanical cues. Yap proteins are well-known transcriptional mechanotransducers, yet their role in gastrulation remains elusive. Here we show that double knockout yap and its paralog yap1b medaka results an failure, due...
Sight depends on the tight cooperation between photoreceptors and pigmented cells, which derive from common progenitors through bifurcation of a single gene regulatory network into neural retina (NR) retinal-pigmented epithelium (RPE) programs. Although genetic studies have identified upstream nodes controlling these networks, their logic remains poorly investigated. Here, we characterize transcriptome dynamics chromatin accessibility in segregating NR/RPE populations zebrafish. We analyze...
The primordium of the vertebrate eye is composed a pseudostratified and apparently homogeneous neuroepithelium, which folds inward to generate bilayered optic cup. During these early morphogenetic events, vesicle patterned along three different axes—proximo-distal, dorso-ventral, naso-temporal—and major domains: neural retina, retinal pigment epithelium (RPE), stalk. These fundamental steps that enable subsequent development functional eye, entail precise coordination among genetic programs....
Yap1/Taz are well-known Hippo effectors triggering complex transcriptional programs controlling growth, survival, and cancer progression. Here we describe yap1b, a new family member with unique activation domain that cannot be phosphorylated by Src/Yes kinases. We show yap1b evolved specifically in euteleosts (i.e. including medaka but not zebrafish) duplication adaptation of yap1. Using DamID-seq generated maps chromatin occupancy for Yap1, Taz (Wwtr1), Yap1b, gastrulating zebrafish...
During vertebrate embryogenesis, the germ layers are patterned by secreted Nodal signals. In classical model, Nodals elicit signaling binding to a complex comprising Type I/II Activin receptors (Acvr) and co-receptor Tdgf1. However, it is currently unclear whether receptor can also affect distribution of themselves through embryo, unknown which putative Acvr paralogs mediate in zebrafish. Here, we characterize three I (Acvr1) four II (Acvr2) homologs show that – except for Acvr1c all...
The ontogeny of the vertebrate retina has been a topic interest to developmental biologists and human geneticists for many decades. Understanding unfolding genetic program that transforms field progenitors' cells into functionally complex multi‐layered sensory organ is formidable challenge. Although classical studies succeeded in identifying key regulators specification, we are far from understanding architecture their gene regulatory networks (GRNs) thus predicting behaviour. A goal our...
Abstract Genetic studies in human and mice have established a dual role for Vsx genes retina development: an early function progenitors’ specification, later requirement bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent functions are also across vertebrates, as mutant models available only mammals. To gain insight into vsx teleosts, we generated vsx1 vsx2 CRISPR-Cas9 double knockouts ( vsxKO ) zebrafish. Our...
ABSTRACT The condensation of the embryo primary axis is a fundamental landmark in establishment vertebrate body plan. Although complex morphogenetic movements directing cell convergence towards midline have been described extensively, little known on how gastrulating cells interpret mechanical cues. Yap proteins are among best characterized transcriptional mechanotransducers, yet their role gastrulation has remained elusive. Here we show that double knockout yap and its paralog yap1b medaka...