A5068062171- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- Neurogenesis and neuroplasticity mechanisms
- Wnt/β-catenin signaling in development and cancer
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Genomics and Chromatin Dynamics
- Retinal Development and Disorders
- Hippo pathway signaling and YAP/TAZ
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Photoreceptor and optogenetics research
- Nerve injury and regeneration
- Animal Behavior and Reproduction
- Spaceflight effects on biology
- Single-cell and spatial transcriptomics
- Retinoids in leukemia and cellular processes
- Fibroblast Growth Factor Research
- Chromosomal and Genetic Variations
- Pluripotent Stem Cells Research
- Neuroendocrine regulation and behavior
- PI3K/AKT/mTOR signaling in cancer
Champalimaud Foundation
2017-2024
Universidad Autónoma de Madrid
1996-2019
Instituto Cajal
2011-2017
Consejo Superior de Investigaciones Científicas
1996-2017
University of Dundee
2001-2005
Wellcome Trust
2001-2002
Centro de Biología Molecular Severo Ochoa
1996-1999
University of Oxford
1999
Abstract The Drosophila eye is patterned by a dorsal-ventral organising centre mechanistically similar to those in the fly wing and vertebrate limb bud. Here we show how this initiated – first event retinal patterning. Early development primordium divided into dorsal ventral compartments. dorsally expressed homeodomain Iroquois genes are true selector for compartment; their expression regulated Hedgehog Wingless. then induced at interface between Iroquois-expressing non- expressing cells...
While many neuronal differentiation genes have been identified, we know little about what determines when and where neurons will form how this process is coordinated with the of neighbouring tissues. In most vertebrates onset takes place in spinal cord a head to tail sequence. Here demonstrate that changing signalling properties adjacent paraxial mesoderm control progression neurogenesis chick cord. We find an inverse relationship between expression caudal neural prospective cord, which...
Coordination between functionally related adjacent tissues is essential during development. For example, formation of trunk neural crest cells (NCCs) highly influenced by the mesoderm, but molecular mechanism involved not well understood. As part this mechanism, fibroblast growth factor (FGF) and retinoic acid (RA) mesodermal gradients control onset neurogenesis in extending tube. In paper, using gain- loss-of-function experiments, we show that caudal FGF signaling prevents premature...
Ruth Diez del Corral, Pilar Aroca, José Luis Gómez-Skarmeta, Florencia Cavodeassi, and Juan Modolell Centro de Biología Molecular Severo Ochoa, Consejo Superior Investigaciones (C.S.I.C.) Científicas Universidad Antónoma Madrid, Cantoblanco, 28049 Spain
Genetic studies in human and mice have established a dual role for Vsx genes retina development: an early function progenitors’ specification, later requirement bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent functions are also across vertebrates, as mutant models available only mammals. To gain insight into vsx teleosts, we generated vsx1 vsx2 CRISPR/Cas9 double knockouts ( KO) zebrafish. Our electrophysiological...
Abstract We used the white gene as an enhancer trap and reporter of chromatin structure. collected white+ transgene insertions presenting a peculiar pigmentation pattern in eye: expression is restricted to dorsal half eye, with clear-cut dorsal/ventral (D/V) border. This D/V stable heritable, indicating that phenotypic reflects positional information developing eye. Localization these transgenes led us identify unique genomic region encompassing 140 kb 69D1–3 subject this effect. contains at...
Vertebrate embryos display a predominant head-to-tail body axis whose formation is associated with the progressive development of post-cranial structures from pool caudal undifferentiated cells. This involves maintenance active FGF signaling in this region as consequence restricted production secreted factor FGF8. FGF8 transcribed specifically precursor and down-regulated cells differentiate embryo extends caudally. We are interested understanding down-regulation its coordination movement...
ABSTRACT The basic organization of somatosensory circuits in the spinal cord is already setup during initial patterning dorsal neural tube. Extrinsic signals, such as Wnt and TGF‐β pathways, activate combinatorial codes transcription factors that are responsible for generating a pattern discrete domains progenitors (dp). These will give rise to distinct interneurons (dI). Wnt/ βcatenin signaling pathway controls specification dp/dI1–3 interneurons. According current model field, Wnt/βcatenin...
Retinoic acid signaling is required at several steps during the development of spinal cord, from specification generic properties to final acquisition neuronal subtype identities, including its role in trunk neural crest development. These functions are associated with production retinoic specific tissues and highly dependent on context. Here, we review defects manipulations, mostly chick mouse models, trying separate different processes where involved highlight common features, such as...
ABSTRACT A prevalent developmental mechanism for the assignment of cell identities is production spatiotemporal concentration gradients extracellular signaling molecules that are interpreted by responding cells. One such systems Shh gradient controls neuronal subtype identity in ventral spinal cord. Using loss and gain function approaches chick mouse embryos, we show here fibroblast growth factor (FGF) pathway required to restrict domains gene expression as neuroepithelial cells become...
Abstract The Tufted1 (Tft1) dominant mutation promotes the generation of ectopic bristles (macrochaetae) in dorsal mesothorax Drosophila. Here we show that Tft1 corresponds to a gain-of-function allele proneural gene amos is associated with chromosomal aberration at 36F-37A. This causes expression large domains lateral-dorsal embryonic ectoderm, which results supernumerary neurons PNS, and notum region third instar imaginal wing, gives rise mesothoracic extra bristles. Revertants Tft1, lack...
From lamprey to monkeys, the organization of descending control locomotion is conserved across vertebrates. Reticulospinal neurons (RSNs) form a bottleneck for commands, receiving innervation from diencephalic and mesencephalic locomotor centres providing drive spinal motor circuits. Given their optical accessibility in early development, larval zebrafish offer unique opportunity study reticulospinal circuitry. In fish, RSNs are small, highly stereotyped, uniquely identifiable group large...
Abstract Genetic studies in human and mice have established a dual role for Vsx genes retina development: an early function progenitors’ specification, later requirement bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent functions are also across vertebrates, as mutant models available only mammals. To gain insight into vsx teleosts, we generated vsx1 vsx2 CRISPR-Cas9 double knockouts ( vsxKO ) zebrafish. Our...