Maurizio Morri
A5017417393- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Mesenchymal stem cell research
- Liver physiology and pathology
- Cancer Cells and Metastasis
- Fibroblast Growth Factor Research
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Neurogenesis and neuroplasticity mechanisms
- RNA and protein synthesis mechanisms
- COVID-19 Clinical Research Studies
- Extracellular vesicles in disease
- RNA Research and Splicing
- Neuroscience and Neuropharmacology Research
- SARS-CoV-2 and COVID-19 Research
- Cancer-related molecular mechanisms research
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- Nerve injury and regeneration
- T-cell and B-cell Immunology
- S100 Proteins and Annexins
- Virology and Viral Diseases
- interferon and immune responses
- Dietary Effects on Health
- Wnt/β-catenin signaling in development and cancer
Chan Zuckerberg Initiative (United States)
2019-2025
Chan Zuckerberg Biohub San Francisco
2021-2025
Altos Labs
2023-2024
Stanford University
2023
Institute of Science and Technology Austria
2018-2021
Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing biology and enabling new insights into the physiology human organs. We created a reference atlas comprising nearly 500,000 cells from 24 different tissues organs, many same donor. This enabled molecular more than 400 types, their distribution across tissues, tissue-specific variation in gene expression. Using multiple single donor identification clonal T between mutation rate B cells,...
The human brain undergoes rapid development at mid-gestation from a pool of neural stem and progenitor cells (NSPCs) that give rise to the neurons, oligodendrocytes, astrocytes mature brain. Functional study these cell types has been hampered by lack precise purification methods. We describe method for prospectively isolating ten distinct NSPC developing using cell-surface markers. CD24−THY1−/lo were enriched radial glia, which robustly engrafted differentiated into all three lineages in...
Early stages of deadly respiratory diseases including COVID-19 are challenging to elucidate in humans. Here, we define cellular tropism and transcriptomic effects SARS-CoV-2 virus by productively infecting healthy human lung tissue using scRNA-seq reconstruct the transcriptional program “infection pseudotime” for individual cell types. predominantly infected activated interstitial macrophages (IMs), which can accumulate thousands viral RNA molecules, taking over 60% transcriptome forming...
Abstract Cell-free RNA from liquid biopsies can be analyzed to determine disease tissue of origin. We extend this concept identify cell types origin using the Tabula Sapiens transcriptomic atlas as well individual atlases in combination with Human Protein Atlas consensus dataset. define type signature scores, which allow inference that contribute cell-free for a variety diseases.
Skeletal stem and progenitor cell populations are crucial for bone physiology. Characterization of these types remains restricted to heterogenous bulk with limited information on whether they unique or overlap previously characterized types. Here we show, through comprehensive functional single-cell transcriptomic analyses, that postnatal long bones mice contain at least two progenitors bona fide skeletal (SSC) characteristics. An early osteochondral SSC (ocSSC) facilitates growth repair,...
Nutrient availability fluctuates in most natural populations, forcing organisms to undergo periods of fasting and re-feeding. It is unknown how dietary changes influence liver homeostasis. Here, we show that a switch from ad libitum feeding intermittent (IF) promotes rapid hepatocyte proliferation. Mechanistically, IF-induced proliferation driven by the combined action systemic FGF15 localized WNT signaling. Hepatocyte during re-feeding re-establishes constant liver-to-body mass ratio, thus...
G-protein-coupled receptors (GPCRs) form the largest receptor family, relay environmental stimuli to changes in cell behavior and represent prime drug targets. Many GPCRs are classified as orphan because of limited knowledge on their ligands coupling cellular signaling machineries. Here, we engineer a library 63 chimeric that contain domains human understudied functionally linked light-sensing domain rhodopsin. Upon stimulation with visible light, identify activation canonical pathways,...
Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives epithelial tissues, it remains unclear whether such instructive activity is also critical mesenchymal migration. Here, we developed light-activated version the receptor Frizzled 7 (Fz7) to analyze how affects anterior axial mesendoderm (prechordal plate, ppl) within zebrafish...
The development of CRISPR-based barcoding methods creates an exciting opportunity to understand cellular phylogenies. We present a compact, tunable, high-capacity Cas12a system called dual acting inverted site array (DAISY). combined high-throughput screening and machine learning predict optimize the 60-bp DAISY barcode sequences. After optimization, top-performing barcodes had ∼10-fold increased capacity relative best random-screened designs performed reliably across diverse cell types....
Corneal organoids are useful tools for disease modeling and tissue transplantation; however, they have not yet been well studied during maturation. We characterized human iPSC-derived corneal at 1, 2, 3, 4 months of development using single-cell RNA sequencing to determine the cellular heterogeneity each stage. found pluripotent cell clusters committed epithelial lineage 1 month; early epithelial, endothelial, stromal markers 2 months; keratocytes as largest population 3 a large months....
Hormones mediate long-range cell communication and play vital roles in physiology, metabolism, health. Traditionally, endocrinologists have focused on one hormone or organ system at a time. Yet, signaling by its very nature connects cells of different organs involves crosstalk hormones. Here, we leverage the organism-wide single transcriptional atlas non-human primate, mouse lemur (Microcebus murinus), to systematically map source target for 84 classes This work uncovers...
ABSTRACT Mouse lemurs are the smallest, fastest reproducing, and among most abundant primates, an emerging model organism for primate biology, behavior, health conservation. Although much has been learned about their physiology Madagascar ecology phylogeny, little is known cellular molecular biology. Here we used droplet- plate-based single cell RNA-sequencing to profile 226,000 cells from 27 mouse lemur organs tissues opportunistically procured four donors clinically histologically...
ABSTRACT Early stages of deadly respiratory diseases such as COVID-19 have been challenging to elucidate due lack an experimental system that recapitulates the cellular and structural complexity human lung while allowing precise control over disease initiation systematic interrogation molecular events at resolution. Here we show healthy slices cultured ex vivo can be productively infected with SARS-CoV-2, tropism virus its distinct dynamic effects on host cell gene expression determined by...
Abstract Cancers evolve not only through the acquisition and clonal transmission of somatic mutations but also by epigenetic mechanisms that modify cell phenotype. Here, we use histology-guided spatial transcriptomics to characterize hepatoblastoma, a childhood liver cancer exhibits significant histologic proliferative heterogeneity despite activating in Wnt/β-catenin pathway. Highly regions with embryonal histology show high expression Wnt target genes, embryonic biliary transcription...
The SARS-CoV-2 pandemic has differentially impacted populations across race and ethnicity. A multi-omic approach represents a powerful tool to examine risk multi-ancestry genomes. We leverage tracking strategy in which we sequence viral host genomes transcriptomes from nasopharyngeal swabs of 1049 individuals (736 positive 313 negative) integrate them with digital phenotypes electronic health records diverse catchment area Northern California. Genome-wide association disaggregated by...
New high-throughput biochemistry techniques complement selection-based approaches and provide quantitative kinetic thermodynamic data for thousands of protein variants in parallel. With these advances, library generation rather than collection has become rate-limiting. Unlike pooled selection approaches, requires mutant libraries which individual sequences are rationally designed, efficiently recovered, sequence-validated, separated from one another, but current strategies unable to produce...
INTRODUCTION: The human brain undergoes rapid development at mid-gestation from a pool of neural stem and progenitor cells (NSPCs) that give rise to the neurons, oligodendrocytes, astrocytes mature brain. Functional study these cell types has been hampered by lack precise purification methods. METHODS: We combine high-dimensional flow cytometry with single transcriptomics comprehensively purify functionally characterize NSPCs developing during mid-gestation. utilize index sorting based on...