A5010456925- CRISPR and Genetic Engineering
- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Drug Transport and Resistance Mechanisms
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Cell Adhesion Molecules Research
- Nitric Oxide and Endothelin Effects
- Bamboo properties and applications
- Virus-based gene therapy research
- Lung Cancer Treatments and Mutations
- Advanced Wireless Communication Techniques
- Plant Taxonomy and Phylogenetics
- Immune cells in cancer
- Cancer Mechanisms and Therapy
- Luminescence and Fluorescent Materials
- Peptidase Inhibition and Analysis
- Advanced Glycation End Products research
- Cancer-related gene regulation
- Neonatal Respiratory Health Research
- Cytokine Signaling Pathways and Interactions
- Histone Deacetylase Inhibitors Research
Jiangxi Agricultural University
2024-2025
University of South China
2009-2024
Stanford University
2020-2022
Zhejiang University
2015-2019
Hunan Cancer Hospital
2019
Hangzhou Xixi hospital
2018
Guangzhou Medical University
2014-2016
Guangzhou Medical University Cancer Hospital
2015-2016
Chang Jung Christian University
2013
National Cheng Kung University
2013
Septic encephalopathy (SE) is a critical factor determining sepsis mortality. Vascular inflammation known to be involved in SE, but the molecular events that lead development of remain unclear. Using time-lapse vivo two-photon laser scanning microscopy, we provide first direct evidence cecal ligation and puncture septic mice induces microglial trafficking sites adjacent leukocyte adhesion on inflamed cerebral microvessels. Our data further demonstrate injury increased chemokine CXCL1 level...
Abstract Gene editing is a powerful tool for genome and cell engineering. Exemplified by CRISPR–Cas, gene could cause DNA damage trigger repair processes that are often error-prone. Such unwanted mutations safety concerns can be exacerbated when altering long sequences. Here we couple microbial single-strand annealing proteins (SSAPs) with catalytically inactive dCas9 editing. This cleavage-free editor, dCas9–SSAP, promotes the knock-in of sequences in mammalian cells. The dCas9–SSAP editor...
Abstract Tumor microenvironment (TME)‐triggered phototheranostic platform offers a feasible strategy to improve cancer diagnosis accuracy and minimize treatment side effects. Developing stable biocompatible molecular for TME‐activated second near‐infrared (NIR‐II) fluorescence imaging‐guided multimodal cascade therapy is promising creating desirable anticancer agents. Herein, new NIR‐II activatable (IR‐FEP‐RGD‐S‐S‐S‐Fc) presented actively targeted tumor imaging hydrogen sulfide (H 2 S)...
CRISPR systems have been proven as versatile tools for site-specific genome engineering in mammalian species. During the gene editing processes, these RNA-guide nucleases introduce DNA double strand breaks (DSBs), which non-homologous end joining (NHEJ) dominates repair pathway, limiting efficiency of homology-directed (HDR), alternative pathway essential precise targeting. Multiple approaches developed to enhance HDR, including chemical compound or RNA interference-mediated inhibition NHEJ...
Dysfunction of neurovascular pericytes underlies breakdown the blood-brain barrier, but molecular mechanisms are largely unknown. In this study, we evaluated role transient receptor potential melastatin-related 2 (TRPM2) channel and autophagy during brain pericyte injury both in vitro vivo.A rapid induction human vascular pericytes, zinc oxide nanoparticles (ZnO-NP)-induced cell stress model, was paralleled with an increase expression TRPM2-S truncated isoform, which abolished by treatment a...
The development of CRISPR-based barcoding methods creates an exciting opportunity to understand cellular phylogenies. We present a compact, tunable, high-capacity Cas12a system called dual acting inverted site array (DAISY). combined high-throughput screening and machine learning predict optimize the 60-bp DAISY barcode sequences. After optimization, top-performing barcodes had ∼10-fold increased capacity relative best random-screened designs performed reliably across diverse cell types....
Based on several evidences from morphology, micromorphology the abaxial leaf epidermis, and phylogenetic analysis by using complete chloroplast genome data, a new combination two synonyms were reported in genus Indocalamus Nakai. cordatus T. H. Wen et Y. Zou was synonymized with I. tessellatus (Munro) P. C. Keng, multinerus (W. Lin Z. M. Wu) W. chebalingensis Lin. As combination, mollis (H. R. Zhao L. Yang) Q. Gao, G. Zhang Yang, created supplementally described. In addition, lectotype of...
Based on several sources of evidence from morphology, the micromorphology abaxial leaf epidermis, and a phylogenetic analysis using complete chloroplast genome data, new combination two synonyms are reported in genus Indocalamus Nakai. cordatus T. H. Wen et Y. Zou is synonymized with I. tessellatus (Munro) P. C. Keng, multinerus (W. Lin Z. M. Wu) W. chebalingensis Lin. As combination, mollis (H. R. Zhao L. Yang) Q. Gao, G. Zhang Yang was created supplementally described. In addition,...
Introduction Meliaceae, a significant group in Sapindales, possesses material and medicinal value due to its applications timber bioactive compounds. However, the high morphological diversity of Meliaceae species lack comparative studies chloroplast (cp) genomes have led great challenges classification identification species. Methods In this study, we sequenced complete cp three Toona ( fargesii , ciliata sinensis ), conducted analyses these along with 29 previously published Meliaceae....
Abstract TCRP-1 (Tongue Cancer Resistance-related Protein-1) gene was recently cloned from the multidrug resistance tongue cancer cell (Tca8113/PYM) which developed by ourselves team (GeneBank accession number: EF36480). Subsequently we found that can mediate cells to specific cisplatin chemotherapy tolerance and radiotherapy tolerance, its mechanism may be caused at least in part TCRP1 induced activation of PI3K/Akt/NF-κB signaling pathway promotes survival. Recently, using real-time...
// Guopei Zheng 1, * , Xiaoting Jia Cong Peng 1 Yingen Deng Jiang Yin Zhijie Zhang Nan Li Min Xiaorong Liu Hao Minying Lu Chengkun Wang Yixue Gu Zhimin He Cancer Hospital and Research Institute of Guangzhou Medical University, 510095, Guangdong, China These authors have contributed equally to this work Correspondence to: He, e-mail: hezhimin2005@yahoo.com Keywords: tongue cancer, miR-491-3p, Rictor, mTORC2, drug resistance Received: October 17, 2014 Accepted: January 18, 2015 Published:...
Abstract Severe hypoglycemia has a detrimental impact on the cerebrovasculature, but molecular events that lead to disruption of integrity tight junctions remain unclear. Here, we report microvessel was dramatically compromised (59.41% wild‐type mice) in TP53‐induced glycolysis and apoptosis regulator (TIGAR) transgenic mice stressed by hypoglycemia. Melatonin, potent antioxidant, protects against hypoglycemic stress‐induced brain endothelial junction injury dosage 400 nmol/L vitro. FRET...
Chemoresistance is a major obstacle in successfully treating cancers, and the mechanisms responsible for drug resistance are still far from understood. Carbonic anhydrase 9 (CA9) has been shown to be upregulated drug-resistant tongue cancer cell line Tca8113/PYM associated with resistance. However, regulating CA9 expression its role remain unclear. Bioinformatic experimental analysis involving ChIP luciferase reporter assays were used validate Zinc finger E-box-binding homeobox 1 (ZEB1) as...
Several existing technologies enable short genomic alterations including generating indels and nucleotide variants, however, engineering more significant changes is challenging due to reduced efficiency precision. Here, we developed RecT Editor via Designer-Cas9-Initiated Targeting (REDIT), which leverages phage single-stranded DNA-annealing proteins (SSAP) for mammalian genome engineering. Relative Cas9-mediated homology-directed repair (HDR), REDIT yielded up a 5-fold increase of insert...
Breast cancer resistance protein (BCRP) has been shown to confer multidrug resistance, but the mechanisms of its regulation are poorly understood. Here, we investigate effects wild‐type and mutant p53, nuclear factor kappa‐B (NF‐κB) (p50) on BCRP promoter activity in MCF‐7 cells. Our results demonstrated that p53 markedly suppressed enhanced chemosensitivity cells mitoxantrone, whereas had little inhibitory effect. After inhibition NF‐κB, similar were obtained. Following knockdown endogenous...
Summary Objective The cholinergic deficit is thought to underlie progressed cognitive decline in Alzheimer Disease. lineage reprogramming of somatic cells into neurons may provide strategies toward cell‐based therapy neurodegenerative diseases. Methods and results Here, we found that a combination neuronal transcription factors, including Ascl1 , Myt1l Brn2, Tlx3, miR124 (5Fs) were capable directly converting human brain vascular pericytes ( HBVP s) cells. Intriguingly, the inducible effect...