A5077123443- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Angiogenesis and VEGF in Cancer
- Cardiac Fibrosis and Remodeling
- Signaling Pathways in Disease
- Cardiomyopathy and Myosin Studies
- Ocular Surface and Contact Lens
- Chromosomal and Genetic Variations
- Psychosomatic Disorders and Their Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Pituitary Gland Disorders and Treatments
- Genomics and Phylogenetic Studies
- Growth Hormone and Insulin-like Growth Factors
- Adrenal and Paraganglionic Tumors
- Corneal surgery and disorders
- Drug-Induced Adverse Reactions
- Hallucinations in medical conditions
- Corneal Surgery and Treatments
- Zebrafish Biomedical Research Applications
- Glaucoma and retinal disorders
- Acute Myeloid Leukemia Research
- Plant Disease Resistance and Genetics
- Acute Lymphoblastic Leukemia research
- Cardiovascular Conditions and Treatments
Stanford University
2019-2023
Stanford Medicine
2023
Palo Alto University
2023
University of Connecticut
2012
Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing biology and enabling new insights into the physiology human organs. We created a reference atlas comprising nearly 500,000 cells from 24 different tissues organs, many same donor. This enabled molecular more than 400 types, their distribution across tissues, tissue-specific variation in gene expression. Using multiple single donor identification clonal T between mutation rate B cells,...
Abstract Cell-free RNA from liquid biopsies can be analyzed to determine disease tissue of origin. We extend this concept identify cell types origin using the Tabula Sapiens transcriptomic atlas as well individual atlases in combination with Human Protein Atlas consensus dataset. define type signature scores, which allow inference that contribute cell-free for a variety diseases.
Abstract Aims Non-compaction cardiomyopathy is a devastating genetic disease caused by insufficient consolidation of ventricular wall muscle that can result in inadequate cardiac performance. Despite being the third most common cardiomyopathy, mechanisms underlying disease, including cell types involved, are poorly understood. We have previously shown endothelial cell-specific deletion chromatin remodeller gene Ino80 results defective coronary vessel development leads to non-compaction...
[Figure: see text].
Most cell fate trajectories during development follow a diverging, tree-like branching pattern, but the opposite can occur when distinct progenitors contribute to same type. During this convergent differentiation, it is unknown if cells ‘remember’ their origins transcriptionally or whether influences behavior. coronary blood vessels of heart develop from two different progenitor sources—the endocardium (Endo) and sinus venosus (SV)—but transcriptional functional differences related origin...
Corneal organoids are useful tools for disease modeling and tissue transplantation; however, they have not yet been well studied during maturation. We characterized human iPSC-derived corneal at 1, 2, 3, 4 months of development using single-cell RNA sequencing to determine the cellular heterogeneity each stage. found pluripotent cell clusters committed epithelial lineage 1 month; early epithelial, endothelial, stromal markers 2 months; keratocytes as largest population 3 a large months....
Teprotumumab has been shown to be effective in the treatment of thyroid eye disease, a potentially vision-threatening condition. Adverse events, including sensorineural hearing loss, have associated with teprotumumab. The authors present case 64-year-old female who discontinued teprotumumab due significant loss after 4 infusions, along other adverse events. patient was unresponsive subsequent course intravenous methylprednisolone and orbital radiation, during which she experienced worsening...
Abstract Chronic traumatic encephalopathy (CTE) is a neurodegenerative disease associated with repetitive head trauma. Brain pathology in CTE characterized by neuronal loss, gliosis, and distinctive pattern of accumulation hyper-phosphorylated tau (p-tau) phospho-TDP43 (p-TDP43). Visual anomalies have been reported patients CTE, but the ocular underlying these symptoms unknown. We evaluated retinal post-mortem eyes collected from 8 contact sport athletes brain autopsy-confirmed stage IV...
Abstract Non-compaction cardiomyopathy is a devastating genetic disease caused by insufficient consolidation of ventricular wall muscle that can result in inadequate cardiac performance. Despite being the third most common cardiomyopathy, mechanisms underlying disease, including cell types involved, are poorly understood. We have previously shown endothelial cell-specific deletion chromatin remodeler gene Ino80 results defective coronary vessel development leads to noncompaction embryonic...
ABSTRACT Regenerating coronary blood vessels has the potential to ameliorate ischemic heart disease, yet there is currently no method of stimulating clinically effective cardiac angiogenesisis. Endocardial cells— a particularly plastic cell type during development—line lumen and are natural vessel progenitors. Their intrinsic angiogenic lost in adults, but studying endocardial- to-coronary developmental pathway could identify methods re-instating this process diseased hearts. Here, we use...
Purpose: This case describes the successful visual restoration of a patient with end-stage Stevens–Johnson syndrome (SJS) severely keratinized ocular surface. Methods: study is report. Results: A 67-year-old man SJS secondary to allopurinol sought rehabilitation options. His surface was compromised from sequelae chronic SJS, leaving him light perception vision bilaterally. The left eye completely severe ankyloblepharon. right had failed penetrating keratoplasty, limbal stem cell deficiency,...
Abstract Coronary artery disease (CAD) is the leading cause of death worldwide, but there are currently no available methods to stimulate growth or regeneration networks in diseased hearts. Studying how arteries built during embryonic development could illuminate strategies for re-building these vessels setting ischemic heart disease. We previously found, using loss-of-function experiments, that transcription factor Dach1 required coronary mouse embryos. Here, we report overexpression...
Phansalkar, Ragini PhD; Navarro, Sylvia E. Villarreal MD; Chiang, Homer Moss, Heather MD, PhDEditor(s): Winges, Kimberly M. Gilhooley, Michael J. MA, MB, BChir, DPhil Author Information
Abstract Most cell fate trajectories during development follow a diverging, tree-like branching pattern, but the opposite can occur when distinct progenitors contribute to same type. During this convergent differentiation, it is unknown if cells “remember” their origins transcriptionally or whether influences behavior. coronary blood vessels of heart develop from two different progenitor sources—the endocardium (Endo) and sinus venosus (SV)—but transcriptional functional differences related...
Regenerating coronary blood vessels has the potential to ameliorate ischemic heart disease, yet there is currently no method of stimulating clinically effective cardiac angiogenesisis. Endocardial cells— a particularly plastic cell type during development—line lumen and are natural vessel progenitors. Their intrinsic angiogenic lost in adults, but studying endocardialto-coronary developmental pathway could identify methods re-instating this process diseased hearts. Here, we use combination...