A5041988687- Single-cell and spatial transcriptomics
- Sarcoma Diagnosis and Treatment
- Soft tissue tumor case studies
- Oral and Maxillofacial Pathology
- Tumors and Oncological Cases
- RNA Research and Splicing
- Neonatal Respiratory Health Research
- Gastroesophageal reflux and treatments
- Esophageal Cancer Research and Treatment
- Cancer Genomics and Diagnostics
- Hernia repair and management
- Corrosion Behavior and Inhibition
- Congenital Anomalies and Fetal Surgery
- Drug-Induced Adverse Reactions
- Fungal Infections and Studies
- Cancer-related molecular mechanisms research
- Semiconductor materials and devices
- Renal and related cancers
- Molecular Biology Techniques and Applications
- Immune cells in cancer
- Hedgehog Signaling Pathway Studies
- Vascular Tumors and Angiosarcomas
- Congenital heart defects research
- Bone Tumor Diagnosis and Treatments
- Pediatric Hepatobiliary Diseases and Treatments
Stanford University
2013-2025
Addenbrooke's Hospital
2024
Palo Alto University
2023
Lucile Packard Children's Hospital
2023
Palo Alto Institute
2022
Stanford Medicine
2017-2021
Lurie Children's Hospital
2019-2020
Northwestern University
2019-2020
University of the Andes
2017
Stanford Health Care
2012-2013
Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing biology and enabling new insights into the physiology human organs. We created a reference atlas comprising nearly 500,000 cells from 24 different tissues organs, many same donor. This enabled molecular more than 400 types, their distribution across tissues, tissue-specific variation in gene expression. Using multiple single donor identification clonal T between mutation rate B cells,...
Macrophages are specialized phagocytic cells, present in all tissues, which engulf and digest pathogens, infected dying debris, can recruit regulate other immune cells the inflammatory response aid tissue repair. Macrophage subpopulations play distinct roles these processes disease, typically recognized by differences marker expression, function, or of residency. Although macrophage brain have been found to developmental origins, extent development contributes diversity tissues within is not...
Drug reaction with eosinophilia and systemic symptoms (DRESS) is a severe, delayed hypersensitivity (DHR). We observed DRESS to inhibitors of interleukin 1 (IL-1) or IL-6 in small group patients Still's disease atypical lung disease. sought characterise features compared drug-tolerant controls. analysed human leucocyte antigen (HLA) alleles for association inhibitor-related DHR, including Kawasaki (KD) cohort.
Abstract Cell-free RNA from liquid biopsies can be analyzed to determine disease tissue of origin. We extend this concept identify cell types origin using the Tabula Sapiens transcriptomic atlas as well individual atlases in combination with Human Protein Atlas consensus dataset. define type signature scores, which allow inference that contribute cell-free for a variety diseases.
Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder associated with defects and situs inversus totalis, the complete mirror image reversal of internal organ (positioning). A variable incidence heterotaxy, or irregular situs, also has been reported in PCD patients, but it not known whether this elicited by PCD-causing genetic lesion. We studied mouse model recessive mutation Dnahc5, dynein gene commonly mutated PCD. Analysis homozygous mutant embryos from 18 litters...
The extent splicing is regulated at single-cell resolution has remained controversial due to both available data and methods interpret it. We apply the SpliZ, a new statistical approach, detect cell-type-specific in >110K cells from 12 human tissues. Using 10X Chromium for discovery, 9.1% of genes with computable SpliZ scores are cell-type-specifically spliced, including ubiquitously expressed MYL6 RPS24 . These results validated RNA FISH, PCR, Smart-seq2. analysis reveals 170 during...
Objective: To quantify liver fibrosis in infants with biliary atresia (BA) through automated analysis of collagen extracellular matrix (ECM) ultrastructure index biopsies and use a composite architecture score to predict native survival. Summary Background Data: Despite early management Kasai portoenterostomy , BA remains the leading indication for pediatric transplantation. There is no established method quantitatively assessing patients BA, factors accurately which will ultimately require...
<title>Abstract</title> Myoepithelial carcinoma is an ultra-rare pediatric solid tumor with no targeted treatments. Clinical implementation of RNA sequencing (RNA-Seq) for identifying therapeutic targets underexplored in cancer. We previously published the Comparative Analysis Expression (CARE), a framework incorporating RNA-Seq-derived gene expression into clinic difficult-to-treat cancers. Here, we discuss 4-year-old male diagnosed myoepithelial who was treated at Stanford Medicine...
Abstract Neuroblastoma (NB) is the most frequent extracranial childhood tumor. The majority of NB tumors arise in adrenal gland with bone marrow (BM) metastases present 70.5% Stage IV disease. A recent study using 23 commonly used two-dimensional (2D) adherent cell lines found that expression GD2, a target for immunotherapy, significantly correlated adrenergic (ADRN) and mesenchymal (MES) lineages through epigenetic regulation ganglioside synthesis enzyme ST8SIA1. We aimed to establish...
Transcription factors (TFs) establish cell identity during development by binding regulatory DNA in a sequence-specific manner, often promoting local chromatin accessibility, and regulating gene expression. Mapping accessible offers critical insights into transcriptional control, but available datasets for human are restricted to bulk tissue, single organs, or modalities. Here, we present the Human Development Multiomic Atlas (HDMA), single-cell atlas of accessibility expression from 817,740...
Self-assembled organic thin films of dodecanethiol (DT), mercaptobenzothiazole (MBT), benzotriazole (BTA), imidazole (IMD) and benzothiazole (BT) are formed by adsorption on the surface copper film used in ultralarge-scale integrated circuits. The characterized x-ray photoelectron spectroscopy. inhibition corrosion these is investigated aerated 0.5 M H2SO4 solutions electrochemical impedance spectroscopy potentiodynamic polarization techniques. presence reduced blocking from oxygen dissolved...
Congenital peribronchial myofibroblastic tumor (CPMT) is a rare benign infantile pulmonary neoplasm that presents prenatally or early in infancy, and exhibits distinctive histologic features characterized by the presence of cartilaginous islands intermixed with bland spindle cells, not uncommonly displaying prominent mitoses. Despite its nature, CPMT can lead to fetal demise, postnatal respiratory distress, complications from perinatal surgical resection. Although morphologic clinical are...
<b><i>Introduction:</i></b> Infertility is common for individuals with differences of sex development (DSD) and a significant concern to these individuals. Fertility potential in many DSD conditions poorly understood. Gonadal tissue cryopreservation (GTC) fertility preservation (FP) offered children cancer undergoing gonadotoxic therapy. Our team sought expand the field FP by offering evaluating success GTC DSD. <b><i>Materials Methods:</i></b>...
Recurrent alterations in receptor tyrosine kinase (RTK) and downstream effectors are described infantile fibrosarcoma (IFS)/cellular congenital mesoblastic nephroma (cCMN) a subset of spindle cell sarcomas, provisionally designated 'NTRK-rearranged' neoplasms. These two groups tumours demonstrate overlapping morphologies harbour NTRK1/2/3, RET, MET, ABL1, ROS1, RAF1 BRAF, although their relationship is not fully elucidated. We describe herein cohort paediatric with clinicopathological...
Context.— Perivascular epithelioid cell tumors (PEComas) are rare mesenchymal of uncertain histogenesis expressing smooth muscle and melanocytic markers. The clinicopathologic spectrum in young patients is not well documented. Objective.— To describe a multi-institutional series PEComas children, adolescents, adults. Design.— PEComas, otherwise specified (NOS); angiomyolipomas (AMLs); lymphangioleiomyomatosis; clear sugar were retrospectively identified from 6 institutions the authors’...
Context.— Pediatric soft tissue tumors are one of the areas pediatric pathology that frequently generate consult requests. Evolving classification systems, ancillary testing methods, new treatment options, research enrollment opportunities, and archival processes create additional complexity in handling these unique specimens. Pathologists at heart this critical decision-making, balancing responsibilities to consider expediency, accessibility, cost-effectiveness during pathologic examination...
Pathological diagnosis of solitary fibrous tumor (SFT) in the pediatric population is challenging, as it occurs uncommonly this age-group and resembles other spindle cell neoplasms. SFT contains a NAB2-STAT6 fusion gene, which can be reliably detected using STAT6 immunohistochemistry. Positive staining highly sensitive specific. We sought to investigate utility immunohistochemistry, show how commonly was historically recognized at 3 academic institutions, reclassify them when appropriate,...