A5101861708- Cancer Genomics and Diagnostics
- Mycobacterium research and diagnosis
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Lung Cancer Treatments and Mutations
- Advanced biosensing and bioanalysis techniques
- Tuberculosis Research and Epidemiology
- CRISPR and Genetic Engineering
- Infectious Diseases and Mycology
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- Respiratory viral infections research
- Cancer-related molecular mechanisms research
- Sarcoidosis and Beryllium Toxicity Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Molecular Biology Techniques and Applications
- Prostate Cancer Treatment and Research
- RNA and protein synthesis mechanisms
- Prostate Cancer Diagnosis and Treatment
- Lung Cancer Diagnosis and Treatment
- Neonatal Respiratory Health Research
- Extracellular vesicles in disease
- RNA Research and Splicing
- PARP inhibition in cancer therapy
Stanford University
2023-2025
University of Missouri
2024
Regeneron (United States)
2024
Seoul National University
2007-2023
Seoul National University Hospital
2019-2023
Yonsei University
2023
Stanford Health Care
2023
EuBiologics (South Korea)
2021
Seoul Metropolitan Government
2019-2020
Boramae Medical Center
2019-2020
Adenosine-to-inosine (A-to-I) RNA editing leads to transcriptome diversity and is important for normal brain function. To date, only a handful of functional sites have been identified in mammals. We developed an unbiased assay screen more than 36,000 computationally predicted nonrepetitive A-to-I using massively parallel target capture DNA sequencing. A comprehensive set several hundred human was detected by comparing genomic with RNAs from seven tissues single individual. Specificity our...
Utilizing the full power of next-generation sequencing often requires ability to perform large-scale multiplex enrichment many specific genomic loci in multiple samples. Several technologies have been recently developed but await substantial improvements. We report 10,000-fold improvement a previously padlock-based approach, and apply assay identifying genetic variations hypermutable CpG regions across human chromosome 21. From ∼3 million reads derived from single Illumina Genome Analyzer...
Abstract Cell-free DNA (cfDNA) sequencing has demonstrated great potential for early cancer detection. However, most large-scale studies have focused only on either targeted methylation sites or whole-genome sequencing, limiting comprehensive analysis that integrates both epigenetic and genetic signatures. In this study, we present a platform enables simultaneous of methylation, copy number, fragmentomic patterns cfDNA in single assay. Using total 950 plasma (361 healthy 589 cancer) 240...
The protein enzyme ribonuclease A (RNaseA) cleaves RNA with catalytic perfection, although little sequence specificity, by a divalent metal ion (M2+)-independent mechanism in which pair of imidazoles provides general acid and base catalysis, while cationic amine electrostatic stabilization the transition state. Synthetic imitation this remarkable organo-catalyst ("RNaseA mimicry") has been longstanding goal biomimetic chemistry. 925-11 DNAzyme contains synthetically modified nucleotides...
Abstract Background Lung adenocarcinoma is a highly heterogeneous disease with various etiologies, prognoses, and responses to therapy. Although genome-scale characterization of lung has been performed, comprehensive somatic mutation analysis EGFR / KRAS ALK -negative in never-smokers not conducted. Methods We analyzed whole exome sequencing data from 16 adenocarcinomas additional 54 tumors two expansion cohort sets. Candidate loci were validated by target capture Sanger sequencing. Gene set...
Abstract We present an in-depth single-cell atlas of in vitro multiculture systems on human primary airway epithelium derived from normal and diseased lungs 27 individual donors. Our large-scale profiling identified new cell states differentiation trajectories rare epithelial types distal lungs. By integrating datasets lung tissues, we discovered immune-primed subsets enriched organoids patients with chronic respiratory disease. To demonstrate the full potential our platform, further...
A large number of Cα−H···O contacts are present in transmembrane protein structures, but contribution such interactions to stability is still not well understood. According previous ab initio quantum calculations, the stabilization energy a contact about 2−3 kcal/mol. However, experimental studies on two different hydrogen bonds proteins lead conclusions that one only weakly stabilizing and other even stabilizing. We note most computational were optimized geometries isolated molecules,...
Abstract Acute exacerbation (AE) significantly affects the prognosis of patients with interstitial lung disease (ILD). This study aimed to investigate best prognostic biomarker for AE-ILD. Clinical data obtained during hospitalization were retrospectively analyzed 96 AE-ILD at three tertiary hospitals. The mean age all subjects was 70.1 years; percentage males 66.7%. Idiopathic pulmonary fibrosis accounted 60.4% cases. During follow-up (median: 88 days), in-hospital mortality 24%....
Traditional tissue-based assessments of genomic alterations in castration-resistant prostate cancer (CRPC) can be challenging. To evaluate the real-world clinical utility liquid biopsies for evaluation CRPC, we preemptively collected available plasma samples and archival tissue from patients that were being treated clinically confirmed CRPC. The cell-free DNA (cfDNA) tumor analyzed using AlphaLiquid®100-HRR panel. Plasma a total 87 included this study. Somatic mutations cfDNA detected 78...
Proteomics and genomics studies have contributed to understanding the pathogenesis of chronic obstructive pulmonary disease (COPD), but previous limitations. Here, using a machine learning (ML) algorithm, we attempted identify pathways in cultured bronchial epithelial cells COPD patients that were significantly affected when exposed cigarette smoke extract (CSE).Small airway collected from with those without who underwent bronchoscopy. After expansion through primary cell culture, treated or...
Poly(ADP-ribose) polymerase inhibitors have been shown dramatic responses in patients with BRCAness. However, clinical studies limited to breast cancer germline mutations. Here, we describe a patient metastatic who had rare <i>BRCA1</i> somatic mutation (<i>BRCA1</i> c.4336G>T (p.E1446*)) detected by cell-free DNA analysis after failing standard therapies. This tier III variant of unknown significance was predicted be pathogenic our assessment, leading us consider...
Abstract Although prime editors are a powerful tool for genome editing, which can generate various types of mutations such as nucleotide substitutions, insertions, and deletions in the without double-strand breaks or donor DNA, conventional still limited to its target scopes because PAM preference spCas9 protein. Here, we described engineered expand range their sites using PAM-flexible Cas9 variants.
Methionine oxidation can substantially alter the structure and functionality of monoclonal antibodies (mAbs), especially when it occurs in complementarity-determining regions (CDRs). It is imperative to fully understand effects methionine because these modifications affect binding affinity, stability, immunogenicity mAbs. Moreover, presence multiple methionines close proximity within amino acid sequence increases complexity accurate characterization, sophisticated analytical methods are...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
A respiratory disorder was noted in a 5-year-old female orangutan kept the Yongin Farmland. Radiographically, multiple radiodense foci ranging from 2 to 6 mm diameter were seen throughout lung lobes. Grossly, thoracic cavity revealed firm texture and grayish-pink discoloration of left apical lobe. Histopathologically, multifocal areas granulomatous pneumonia present right Both primers IS 1081 IS6110 targeting 196 bp 245 respectively used polymerase chain reaction, Mycobacterium tuberculosis...
Genetic changes in Mycobacterium abscessus during antibiotic treatment are not fully understood. This study aimed to investigate the genetic M. patients receiving treatment, and their clinical implications. Pretreatment 12-month post-treatment isolates were obtained from with pulmonary disease. Isolates each time point separated into six groups based on distinctive morphological characteristics. Twenty-four isolates, comprising 12 patient A exhibiting progressive disease B demonstrating...
Abstract Background : Nontuberculous mycobacterium (NTM) species are ubiquitous microorganisms. NTM pulmonary disease (NTM-PD) is thought to be caused not by human-to-human transmission but independent environmental acquisition. However, recent studies using next-generation sequencing (NGS) have reported trans-continental spread of Mycobacterium abscessus among patients with cystic fibrosis. Results We investigated genomes through NGS examine patterns in three pairs co-habiting NTM-PD who...
Abstract Background: Lung adenocarcinoma is a highly heterogeneous disease with various etiologies, prognoses, and responses to therapy. Although genome-scale characterization of lung has been performed, somatic mutation analysis never-smoker EGFR/KRAS/ALK-negative not comprehensively conducted. Methods: Patients were eligible if they never smoker without EGFR/KRAS ALK rearrangement. Whole exome sequencing was performed using the Illumina HiSeq 2,000 platform. Candidate loci identified in...
Abstract Background: The extent to which metastatic tumors further evolve by accumulating additional mutations is unclear and has yet be systematically addressed using next-generation sequencing in high-grade serous ovarian cancer. Methods: During cytoreductive surgery prior chemotherapy, 11 spatially separated tumor samples from primary associated sites with 2 normal were obtained. Whole exome copy number analysis performed multiplex PCR followed ultra-deep resequencing was used for...