A5003177600- Extracellular vesicles in disease
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Nanoplatforms for cancer theranostics
- T-cell and B-cell Immunology
- Circular RNAs in diseases
- Cancer Cells and Metastasis
- Neonatal Respiratory Health Research
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Gene Regulatory Network Analysis
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Alzheimer's disease research and treatments
- Video Coding and Compression Technologies
- Chemokine receptors and signaling
- Histone Deacetylase Inhibitors Research
- Medicinal Plants and Neuroprotection
- Advanced Data Compression Techniques
- Hair Growth and Disorders
- Image and Video Quality Assessment
Wellcome Sanger Institute
2023-2024
Seoul National University
2004-2024
Seoul National University Hospital
2021-2024
New Generation University College
2023
National University College
2023
Thyroid hormone (TH) imbalance is linked to the pathophysiology of reversible dementia and Alzheimer’s disease (AD). It unclear whether tissue hypothyroidism occurs in AD brain how it affects on pathology. We find that decreased iodothyronine deiodinase 2 correlated with hippocampal early model mice before TH alterations blood. deficiency leads spontaneous activation microglia wild-type under nonstimulated conditions, resulting lowered innate immune responses response inflammatory stimuli or...
Abstract Microglia play a crucial role in synaptic elimination by engulfing dystrophic neurons via triggering receptors expressed on myeloid cells 2 (TREM2). They are also involved the clearance of beta‐amyloid (Aβ) plaques Alzheimer's disease (AD); nonetheless, driving force behind TREM2‐mediated phagocytosis remains unknown. Here, using advanced 2D/3D/4D co‐culture systems with loss‐of‐function mutations TREM2 (a frameshift mutation engineered exon 2) brain organoids/microglia/assembloids,...
Abstract Gastric cancer (GC) is commonly treated by chemotherapy using 5-fluorouracil (5-FU) derivatives and platinum combination, but predictive biomarker remains lacking. We develop patient-derived xenografts (PDXs) from 31 GC patients treat with a combination of 5-FU oxaliplatin, to determine biomarkers associated responsiveness. When the PDXs are defined as either responders or non-responders according tumor volume change after treatment, responsiveness significantly consistent...
Abstract The maintenance of genetic integrity is critical for stem cells to ensure homeostasis and regeneration. Little known about how adult respond irreversible DNA damage, resulting in loss regeneration humans. Here, we establish a permanent model using cycling human hair follicles treated with alkylating agents: busulfan followed by cyclophosphamide. We uncover the underlying mechanisms which follicle (HFSCs) lose their pool. In contrast immediate destructive changes rapidly...
Abstract We present an in-depth single-cell atlas of in vitro multiculture systems on human primary airway epithelium derived from normal and diseased lungs 27 individual donors. Our large-scale profiling identified new cell states differentiation trajectories rare epithelial types distal lungs. By integrating datasets lung tissues, we discovered immune-primed subsets enriched organoids patients with chronic respiratory disease. To demonstrate the full potential our platform, further...
Fragmentomics, the investigation of fragmentation patterns cell-free DNA (cfDNA), has emerged as a promising strategy for early detection multiple cancers in field liquid biopsy. However, clinical application this approach been hindered by limited understanding cfDNA biology. Furthermore, prevalence hematopoietic cell-derived plasma complicates vivo tissue-specific other than that origin. While conventional two-dimensional cell lines have contributed to research on biology, their...
Abstract Single-cell data analysis can infer dynamic changes in cell populations, for example across time, space or response to perturbation. To compare these dynamics between two conditions, trajectory alignment via programming (DP) optimization is frequently used, but limited by assumptions such as a definite existence of match. Here we describe Genes2Genes , Bayesian information-theoretic DP framework aligning single-cell trajectories. overcomes current limitations and able capture...
Abstract Initial cellular events in a transitional period from normal to cancer cells, such as transcriptomic or epigenetic dynamics, are poorly understood. Observing these is more challenging than progression, especially for humans. Mapping this phenomenon early evolution crucial, we can identify targetable molecules inhibit development at the precancerous stage. Immortalized human cell lines hardly capture appropriately they have lost usual characteristics. Normal organoids harbor stem...
Diffuse intrinsic pontine glioma (DIPG) is one of the most lethal childhood brain tumors. This tumor unique because it detected exclusively in ventral pons patients aged between 6 and 7 years, which suggests a developmental nature its formation. Signal transducer activator transcription 3 (STAT3) critical molecule for differentiation neural stem cells into astrocytes during neurodevelopment. Additionally, STAT3 associated with oncogenesis epithelial‑mesenchymal transition (EMT) various types...
Abstract Patients with triple-negative breast cancer (TNBC) often develop metastases in visceral organs including the liver, but detailed molecular mechanisms of TNBC liver metastasis is not clearly understood. In this study, we tried to dissect process premetastatic niche formation by using patient-derived xenograft (PDX) models different metastatic propensity. RNA sequencing PDX that successfully metastasized showed upregulation Cx3cr1 gene microenvironment. syngeneic models, preceded...
Abstract Vertical sleeve gastrectomy (VSG) is the most commonly performed bariatric/metabolic surgery, exhibiting a high rate of diabetes remission in humans. To elucidate molecular mechanisms VSG, we transcriptomic analysis liver, fat, and muscle VSG mice. C57BL/6 mice fed high-fat diet were randomly assigned to sham or surgery. The sham-operated ad libitum (sham group) pair-fed (sham-PF matching their food intake VSG-operated Comparative using RNA sequencing was performed. reduced body...
Patients with triple-negative breast cancer (TNBC) have an increased risk of distant metastasis compared to those other subtypes. In this study, we aimed identify the genes associated in TNBC and their underlying mechanisms.We established patient-derived xenograft (PDX) models using surgically resected tissues from 31 patients TNBC. Among these, 15 subsequently developed metastases. Candidate metastasis-associated were identified RNA sequencing. vitro wound healing, proliferation, migration,...
Malignant phyllodes tumour (MPT) is a rare breast malignancy with epithelial and mesenchymal features. Currently, there are no appropriate research models or effective targeted therapeutic approaches for MPT.We collected fresh frozen tissues from nine patients MPT performed whole-exome RNA sequencing. Additionally, we established patient-derived xenograft (PDX) tested the efficacy of targeting dysregulated pathways in using PDX model one MPT.MPT has unique molecular characteristics when...
<div>Abstract<p>Triple negative breast cancer (TNBC) patients often develop metastases in visceral organs including the liver but detailed molecular mechanisms of TNBC metastasis is not clearly understood. In this study, we tried to dissect process pre-metastatic niche formation by using patient-derived xenograft (PDX) models with different metastatic propensity. RNA sequencing PDX that successfully metastasized showed up-regulation Cx3cr1 gene microenvironment. syngeneic models,...
Glioblastoma (GBM) has a fatal prognosis because of its aggressive and invasive characteristics. Understanding the mechanism invasion necessitates an elucidation relationship between tumor cells microenvironment. However, there been scarcity suitable models to investigate this. In this study, we established glioblastoma‐cerebral organoid assembloid (GCOA) model by co‐culturing patient‐derived GBM tumoroids human cerebral organoids. Tumor from infiltrated organoids, mimicking nature parental...
Tregs play a central role in maintaining immune tolerance. Recent progress the clinical application of underscores their potential for cell therapy. Nevertheless, notable hurdle remains producing functional
With video compression standards such as MPEG-4, a transmission error happens in video-packet basis, rather than macroblock basis. In this context, we propose semantic prioritization method that determines the size of packet based on importance its contents. A length is made to be short for an important area facial order reduce possibility accumulation. To facilitate prioritization, efficient hardware algorithm face tracking proposed. The increase complexity minimal because motion estimation...
<div>Abstract<p>Patients with triple-negative breast cancer (TNBC) often develop metastases in visceral organs including the liver, but detailed molecular mechanisms of TNBC liver metastasis is not clearly understood. In this study, we tried to dissect process pre-metastatic niche formation by using patient-derived xenograft (PDX) models different metastatic propensity. RNA sequencing PDX that successfully metastasized showed upregulation <i>Cx3cr1</i> gene...
<div>Abstract<p>Triple negative breast cancer (TNBC) patients often develop metastases in visceral organs including the liver but detailed molecular mechanisms of TNBC metastasis is not clearly understood. In this study, we tried to dissect process pre-metastatic niche formation by using patient-derived xenograft (PDX) models with different metastatic propensity. RNA sequencing PDX that successfully metastasized showed up-regulation Cx3cr1 gene microenvironment. syngeneic models,...