A5065444262- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Pharmaceutical and Antibiotic Environmental Impacts
- Tuberculosis Research and Epidemiology
- Lymphoma Diagnosis and Treatment
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
- Drug Transport and Resistance Mechanisms
- Vascular Tumors and Angiosarcomas
- Enterobacteriaceae and Cronobacter Research
- Cancer therapeutics and mechanisms
- Influenza Virus Research Studies
- Pneumonia and Respiratory Infections
- Mycobacterium research and diagnosis
- Chemical Reactions and Isotopes
- Bacterial Infections and Vaccines
- Gut microbiota and health
- Cardiac tumors and thrombi
- Antibiotics Pharmacokinetics and Efficacy
- Evolution and Genetic Dynamics
- Infections and bacterial resistance
- Bacterial biofilms and quorum sensing
- HIV/AIDS Research and Interventions
- Bacterial Identification and Susceptibility Testing
Harvard University
2025
Boston Children's Hospital
2025
Sungkyunkwan University
2014-2024
Samsung Medical Center
2014-2021
ABSTRACT Antibiotic-resistant Gram-negative bacteria remain a globally leading cause of bacterial infection-associated mortality, and it is imperative to identify novel therapeutic strategies. Recently, the advantage using antibacterials selective against has been demonstrated with polymyxins that specifically target lipopolysaccharides bacteria. However, severe cytotoxicity limits their clinical use. Here, we demonstrate polymyxin B nonapeptide (PMBN), derivative without terminal amino acyl...
Abstract Background This study aimed to investigate the population heterogeneity on carbapenem susceptibility in Klebsiella pneumoniae strains that acquired a bla OXA-232 -bearing ColE-type plasmid. Methods A plasmid was electroporated into two carbapenem-susceptible K. strains. High- and low-carbapenem-resistant subpopulations were identified isolated using patch plating. The subsequently subcultured antibiotic-free media, yielding distinct populations: stable, high-level...
Acinetobacter baumannii is one of the most important nosocomial pathogens, and thus it required to investigate how disseminate in hospitals infect patients. We performed whole genome sequencing for 24 A. strains isolated successively from blood a single patient evaluate whether repeated infections were due re-infection or relapse infection within-host evolution. The first strain, BL1, was sequenced de novo using PacBio RSII system. BL2–BL24, with an Illumina Hiseq4000 mapped sequences BL1....
Background.Klebsiella variicola was once recognised as a benign plant-endosymbiont but recent case reports suggest that it is newly emerging Gram-negative pathogen related to opportunistic infection of multiple sites in humans.Methods. Antimicrobial susceptibility testing performed using broth microdilution method. To identify colistin resistance mechanisms, phoPQ, pmrAB, and mgrB were sequenced their mRNA expression analysed quantitative real-time PCR. In addition, we tried detect crrAB...
Heteroresistance, frequently observed in diverse bacterial species, imposes clinical challenges. For this study, we investigated the stability and resilience of tigecycline heteroresistance Acinetobacter baumannii. Four tigecycline-heteroresistant (HR) A. baumannii strains resistant populations (RPs) obtained from them were subjected to laboratory evolution assays for 30 days antibiotic-free media. The phenotype was determined using a population analysis. Bacterial growth curves vitro...
Genetic changes in Mycobacterium abscessus during antibiotic treatment are not fully understood. This study aimed to investigate the genetic M. patients receiving treatment, and their clinical implications. Pretreatment 12-month post-treatment isolates were obtained from with pulmonary disease. Isolates each time point separated into six groups based on distinctive morphological characteristics. Twenty-four isolates, comprising 12 patient A exhibiting progressive disease B demonstrating...
Since the introduction of pneumococcal conjugate vaccine, an increase in incidence Streptococcus pneumoniae serotype 19A and sequence type 320 (19A-ST320) isolates have been observed worldwide including South Korea. We conducted a genome-wide analysis to investigate temporal genetic changes 26 penicillin-non-susceptible 19A-ST320 from hospital Korea over period 17 years (1999; 2004 2015). Although strains were isolated single showed same genotype serotype, whole-genome sequencing (WGS)...
Tigecycline is known to form a complex with divalent cations such as Mg2+ and Ca2+, which essential for mode of action tigecycline. In this study, we investigated the effects levels on in vitro antibacterial activity tigecycline against two strains each Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii. When or Ca2+ was added be tantamount criteria severe hypermagnesemia hypercalcemia Mueller-Hinton II broth, minimum inhibitory concentrations were...
Abstract We identified two Klebsiella variicola isolates from fecal samples: one is colistin-resistant, and another colistin-susceptible. The colistin-resistant K. isolate showed no mutations in phoPQ, pmrAB , mgrB crrAB mcr were not identified. However, its phoQ pbgP expression was significantly higher amino-arabinosylated lipid A with hexa-acylated species lipopolysaccharide