A5034531977- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Advanced Polymer Synthesis and Characterization
- Hydrogels: synthesis, properties, applications
- Tissue Engineering and Regenerative Medicine
- Silicone and Siloxane Chemistry
- Knee injuries and reconstruction techniques
- Supramolecular Chemistry and Complexes
- Hippo pathway signaling and YAP/TAZ
- Tendon Structure and Treatment
- Bone Tissue Engineering Materials
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Polymer Surface Interaction Studies
- Bone fractures and treatments
- Silk-based biomaterials and applications
- Nanoparticle-Based Drug Delivery
- Immune cells in cancer
- Chemical Synthesis and Analysis
- Pluripotent Stem Cells Research
- Extracellular vesicles in disease
Stanford University
2015-2025
Palo Alto University
2017-2024
Stanford Medicine
2015-2017
Tsinghua University
2010-2014
Beijing Institute of Technology
2007-2010
Glioblastoma (GBM) is the most common and aggressive form of primary brain tumor with a median survival 12-15 months, mechanisms underlying GBM progression remain largely elusive. Given importance niche signaling in driving progression, there strong need to develop vitro models facilitate analysis cell-niche interactions physiologically relevant controllable manner. Here we report development bioengineered 3D model help elucidate effects matrix stiffness on cell fate using...
Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for hyaline cartilage regeneration. However, current approaches chondrogenic differentiation of hiPSCs are complicated and inefficient primarily due to intermediate embryoid body formation, which is required generate endodermal, ectodermal, mesodermal cell lineages. We report a new, straightforward highly efficient approach hiPSCs, avoids formation. differentiated directly into mesenchymal /stromal (MSC)...
Diabetes mellitus (DM) is a metabolic disease frequently associated with impaired bone healing. Despite its increasing prevalence worldwide, the molecular etiology of DM-linked skeletal complications remains poorly defined. Using advanced stem cell characterization techniques, we analyzed intrinsic and extrinsic determinants mouse (mSSC) function to identify specific mSSC niche-related abnormalities that could impair repair in diabetic (Db) mice. We discovered high serum concentrations tumor...
Poly(ethylene glycol) hydrogel crosslinking mechanism has a differential effect on controlling protein release.
Zonal organization plays an important role in cartilage structure and function, whereas most tissue-engineering strategies developed to date have only allowed the regeneration of with homogeneous biochemical mechanical cues. To better restore tissue there is a strong need engineer materials biomimetic gradient niche cues that recapitulate native organization. address this critical unmet need, study, we report method for rapid formation tissue-scale hydrogels as three-dimensional (3D) cell...
The development of a sliding hydrogel with mobile crosslinks and biochemical ligands as 3D stem cell niche is reported. molecular mobility this allows cells to reorganize the surrounding change their morphology in 3D. Without changing matrix stiffness, hydrogels support efficient differentiation toward multiple lineages including adipogenesis, chondrogenesis, osteogenesis.
Adipose-derived stromal cells (ADSCs) are attractive autologous cell sources for cartilage repair given their relative abundance and ease of isolation. Previous studies have demonstrated the potential extracellular matrix (ECM) molecules as three-dimensional (3D) scaffolds promoting chondrogenesis. However, few compared effects varying types or doses ECM on chondrogenesis ADSCs in 3D. Furthermore, increasing molecule concentrations often result simultaneous changes stiffness, which makes it...
An ATRA based strategy to synthesize vinyl copolymers with monomer-level sequence control is proposed. In each cycle, one allyl alcohol added the ATRP chain end, and then hydroxymethyl residue oxidized carboxylic acid a side group introduced via esterification, making new end active for next cycle.
Hydrogels are widely used as three-dimensional (3D) tissue engineering scaffolds due to their tissue-like water content, well tunable physical and chemical properties. Hydrogel-based generally associated with nanoscale porosity, whereas macroporosity is highly desirable facilitate nutrient transfer, vascularization, cell proliferation matrix deposition. Diverse techniques have been developed for introducing into hydrogel-based scaffolds. However, most of these methods involve harsh...
Cell niche is a multifactorial environment containing complex interactions between biochemical and physical cues. Although extensive studies have examined the effects of or cues alone on cell fate, how mechanical signals interact to influence fates remains largely unknown. To address this challenge, here we report polyethylene glycol-based gradient hydrogel platform as biomimetic independently tunable matrix stiffness ligand density. The versatility demonstrated by fabricating characterizing...
Abstract Poly(ethylene glycol) (PEG)‐based hydrogels are popular biomaterials for protein delivery to guide desirable cellular fates and tissue repair. However, long‐term release from PEG‐based remains challenging. Here, we report a hydrogel platform long term release, which allows efficient loading of proteins via physical entrapment. Tuning degradation led increase in mesh size gradual over 60 days with retained bioactivity. Importantly, this does not require the chemical modification...
Different tissue types are characterized by varying stiffness and biochemical ligands. Increasing substrate has been shown to trigger Yes-associated protein (YAP) translocation from the cytoplasm nucleus, yet role of ligand density in modulating mechanotransduction stem cell fate remains largely unexplored. Using polyacrylamide hydrogels coated with fibronectin as a model platform, we showed that stiffness-induced YAP occurs only at intermediate densities. At low or high densities,...
Rationale: Injectable matrices are highly desirable for stem cell delivery.Previous research has highlighted the benefit of scaffold macroporosity in enhancing survival and bone regeneration vivo.However, there remains a lack injectable situ crosslinkable macroporous delivery to achieve fast immunocompetent animal model.The goal this study is develop an gelatin-based μRB hydrogel supporting direct encapsulation that available clinics as enhance adipose-derived stromal (ASC) survival,...
Sexually dimorphic tissues are formed by cells that regulated sex hormones. While a number of systemic hormones and transcription factors known to regulate proliferation differentiation osteoblasts osteoclasts, the mechanisms determine sexually differences in bone regeneration unclear. To explore how regeneration, we compared fracture repair between adult male female mice. We found skeletal stem cell (SSC) mediated mice is dependent on estrogen signaling but SSCs from do not exhibit similar...
Glioblastoma multiforme (GBM) is the most prevalent and aggressive brain tumor in adults. Hydrogels have been employed as 3D vitro culture models to elucidate how matrix cues such stiffness degradation drive GBM progression drug responses. Recently, viscoelasticity has identified an important niche cue regulating stem cell differentiation morphogenesis 3D. Brain a viscoelastic tissue, yet modulates fate response remains largely unknown. Using dynamic hydrazone crosslinking chemistry,...