A5038986662- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Tendon Structure and Treatment
- Barrier Structure and Function Studies
- Neurogenesis and neuroplasticity mechanisms
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Congenital Diaphragmatic Hernia Studies
- Neonatal Respiratory Health Research
- Knee injuries and reconstruction techniques
- Congenital Anomalies and Fetal Surgery
- Micro and Nano Robotics
- 3D Printing in Biomedical Research
- Shoulder Injury and Treatment
- Molecular Communication and Nanonetworks
Massachusetts Institute of Technology
2022-2024
Stanford University
2019-2022
Princeton University
2021
Different tissue types are characterized by varying stiffness and biochemical ligands. Increasing substrate has been shown to trigger Yes-associated protein (YAP) translocation from the cytoplasm nucleus, yet role of ligand density in modulating mechanotransduction stem cell fate remains largely unexplored. Using polyacrylamide hydrogels coated with fibronectin as a model platform, we showed that stiffness-induced YAP occurs only at intermediate densities. At low or high densities,...
Abstract Patient-specific, human-based cellular models integrating a biomimetic blood-brain barrier (BBB), immune, and myelinated neuron components are critically needed to enable accelerated, translationally relevant discovery of neurological disease mechanisms interventions. By engineering novel brain-mimicking 3D hydrogel co-culturing all six major brain cell types derived from patient iPSCs, we have constructed, characterized, utilized multicellular integrated (miBrain)...
Injuries of the bone-to-tendon interface, such as rotator cuff and anterior cruciate ligament tears, are prevalent musculoskeletal injuries, yet effective methods for repair remain elusive. Tissue engineering approaches that use cells biomaterials offer a promising potential solution bone–tendon but previous strategies require seeding multiple cell types multiphasic scaffolds to achieve zonal-specific tissue phenotype. Furthermore, mimicking aligned morphology present in native interface...
Type I collagen is the most abundant extracellular matrix protein in human body and commonly used as a biochemical ligand for hydrogel substrates to support cell adhesion mechanotransduction studies. Previous protocols conjugating have different solvents; yet, how varying solvent pH composition impacts efficiency distribution of these coatings remains unknown. Here, we examine effect type on polyacrylamide hydrogels. We further evaluate effects mesenchymal stem cells (MSCs) by characterizing...
<title>Abstract</title> Patient-specific, human-based cellular models integrating a biomimetic blood-brain barrier (BBB), immune, and myelinated neuron components are critically needed to enable accelerated, translationally relevant discovery of neurological disease mechanisms interventions. By engineering novel brain-mimicking 3D hydrogel co-culturing all six major brain cell types derived from patient iPSCs, we have constructed, characterized, utilized multicellular integrated (miBrain)...
Mechanical forces are increasingly recognized as important determinants of cell and tissue phenotype also appear to play a critical role in organ development. During the fetal stages lung morphogenesis, pressure fluid within lumen airways is higher than that chest cavity, resulting positive transpulmonary pressure. Several congenital defects decrease or reverse across developing associated with reduced number branches correspondingly underdeveloped insufficient for gas exchange after birth....
Abstract The ability to engineer complex multicellular systems has enormous potential inform our understanding of biological processes and disease alter the drug development process. Engineering living emulate natural or incorporate new functions relies on a detailed biochemical, mechanical, other cues between cells their environment that result in coordinated action systems. On April 3–6, 2022, experts field met at Keystone symposium “Engineering Multicellular Living Systems” discuss recent...