A5029991842- Neonatal Respiratory Health Research
- Congenital heart defects research
- Congenital Diaphragmatic Hernia Studies
- Cancer Cells and Metastasis
- Computational Drug Discovery Methods
- Bacterial biofilms and quorum sensing
- Renal and related cancers
- Biosensors and Analytical Detection
- Fibroblast Growth Factor Research
- Cellular Mechanics and Interactions
- Advanced Numerical Analysis Techniques
- Tissue Engineering and Regenerative Medicine
- Cytomegalovirus and herpesvirus research
- Congenital Anomalies and Fetal Surgery
- Photoreceptor and optogenetics research
- Ocular Infections and Treatments
Princeton University
2017-2022
Johnson University
2017-2022
Rutgers, The State University of New Jersey
2013-2022
Mechanical forces are increasingly recognized to regulate morphogenesis, but how this is accomplished in the context of multiple tissue types present within a developing organ remains unclear. Here we use bioengineered “microfluidic chest cavities” precisely control mechanical environment fetal lung. We show that transmural pressure controls airway branching frequency smooth muscle contraction, and rate developmental maturation lungs, as assessed by transcriptional analyses. Timelapse...
Smooth muscle guides the morphogenesis of several epithelia during organogenesis, including mammalian airways. However, it remains unclear how airway smooth differentiation is spatiotemporally patterned and whether originates from transcriptionally distinct mesenchymal progenitors. Using single-cell RNA-sequencing embryonic mouse lungs, we show that pulmonary mesenchyme contains a continuum cell identities, but no Transcriptional variability correlates with spatially sub-epithelial...
During development, the mammalian lung undergoes several rounds of branching, rate which is tuned by relative pressure fluid within lumen lung. We carried out bioinformatics analysis RNA-sequencing embryonic mouse lungs cultured under physiologic or sub-physiologic transmural and identified transcription factor-binding motifs near genes whose expression changes in response to pressure. Surprisingly, we found retinoic acid (RA) receptor binding sites significantly overrepresented promoters...
Mechanical forces are increasingly recognized as important determinants of cell and tissue phenotype also appear to play a critical role in organ development. During the fetal stages lung morphogenesis, pressure fluid within lumen airways is higher than that chest cavity, resulting positive transpulmonary pressure. Several congenital defects decrease or reverse across developing associated with reduced number branches correspondingly underdeveloped insufficient for gas exchange after birth....
Abstract Smooth muscle guides morphogenesis of several epithelia during organogenesis, including the mammalian airways. However, it remains unclear how airway smooth differentiation is spatiotemporally patterned and whether originates from distinct mesenchymal progenitors. Using single-cell RNA-sequencing embryonic mouse lungs, we show that pulmonary mesenchyme contains a continuum cell identities, but no Transcriptional variability correlates with sub-epithelial sub-mesothelial compartments...