A5048778639- Redox biology and oxidative stress
- 3D Printing in Biomedical Research
- Computational Drug Discovery Methods
- Cell Image Analysis Techniques
- Cancer, Hypoxia, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Single-cell and spatial transcriptomics
- Gene Regulatory Network Analysis
- Pluripotent Stem Cells Research
- Genetics, Bioinformatics, and Biomedical Research
- Metabolomics and Mass Spectrometry Studies
- Bioactive Compounds and Antitumor Agents
- T-cell and B-cell Immunology
- Glutathione Transferases and Polymorphisms
- Bioinformatics and Genomic Networks
- Free Radicals and Antioxidants
- CAR-T cell therapy research
- Microfluidic and Bio-sensing Technologies
- bioluminescence and chemiluminescence research
- Planarian Biology and Electrostimulation
- Cancer Cells and Metastasis
- Electron Spin Resonance Studies
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Nanoparticles: synthesis and applications
Georgia Institute of Technology
2016-2025
The Wallace H. Coulter Department of Biomedical Engineering
2016-2025
Emory University
2010-2024
Indian River State College
2019-2024
Cancer Institute (WIA)
2022
AID Atlanta
2015-2019
Bioengineering Center
2015
International Society for Optics and Photonics
2015
University of Victoria
2015
Stanford University
2012
Resistance to ionizing radiation, a first-line therapy for many cancers, is major clinical challenge. Personalized prediction of tumor radiosensitivity not currently implemented clinically due insufficient accuracy existing machine learning classifiers. Despite the acknowledged role metabolism in radiation response, metabolomics data rarely collected large multi-omics initiatives such as The Cancer Genome Atlas (TCGA) and consequently omitted from algorithm development. In this study, we...
Hydrogen peroxide is appreciated as a cellular signaling molecule with second-messenger properties, yet the mechanisms by which cell protects against intracellular H(2)O(2) accumulation are not fully understood. We introduce network model of clearance that includes pseudo-enzymatic oxidative turnover protein thiols, enzymatic actions catalase, glutathione peroxidase, peroxiredoxin, and glutaredoxin, redox reactions thioredoxin glutathione. Simulations reproduced experimental observations...
Recent technological breakthroughs in our ability to derive and differentiate induced pluripotent stem cells, organoid biology, organ-on-chip assays, 3-D bioprinting have all contributed a heightened interest the design, assembly, manufacture of living systems with broad range potential uses. This white paper summarizes state emerging field "multi-cellular engineered systems," which are composed interacting cell populations. accomplishments described, focusing on current applications, as...
Stochasticity in gene expression, protein or metabolite levels contributes to cell–cell variations, the analysis of which could lead a better understanding cellular processes and drug responses. Current technologies are limited their throughput, resolution (in space, time, tracking individual cells instead population average) ability control environment. A few microfluidic tools have been developed trap image cells; however, most designs available date, there is compromise among loading...
Preneoplastic lesions carry many of the antigenic targets found in cancer cells but often exhibit prolonged dormancy. Understanding how host response to premalignancy is maintained and altered during malignant transformation needed prevent cancer. To understand immune microenvironment precursor monoclonal gammopathy undetermined significance (MGUS) myeloma, we analyzed bone marrow from 12 healthy donors 26 patients with MGUS/myeloma by mass cytometry concurrently profiled transcriptomes...
Titanium dioxide nanoparticles (TiO2 NPs), used as pigments and photocatalysts, are ubiquitous in our daily lives. Previous work has observed cellular oxidative stress response to the UV-excitation of photocatalytic TiO2 NPs. In comparison, most human exposure NPs takes place dark, lung following inhalation or gut consumption NP food pigment. Our spectroscopic characterization shows that both grade NPs, generate low levels reactive oxygen species (ROS), specifically hydroxyl radicals...
Abstract Proximal signaling events activated by TCR-peptide/MHC (TCR-pMHC) binding have been the focus of intense ongoing study, but understanding how consequent downstream networks integrate to govern ultimate avidity-appropriate TCR-pMHC T cell responses remains a crucial next challenge. We hypothesized that quantitative combination key network signals across multiple pathways must encode information generated TCR activation, providing basis for model capable interpreting and predicting...
Current management of childhood leukemia is tailored based on disease risk determined by clinical features at presentation. Whether properties the host immune response impact and outcome not known. Here, we combine mass cytometry, single cell genomics, functional studies to characterize BM environment in children with B acute lymphoblastic myelogenous T cells marrow demonstrate evidence chronic activation exhaustion/dysfunction, attrition naive TCF1+ stem-like memory accumulation terminally...
Mesenchymal stem/stromal cell (MSC) therapies have had limited success so far in clinical trials due part to heterogeneity immune-responsive phenotypes. Therefore, techniques characterize these properties of MSCs are needed during biomanufacturing. Imaging shape, or morphology, has been found be associated with MSC immune responsivity-but a direct relationship between single-cell morphology and function not established. We used label-free differential phase contrast imaging matrix-assisted...
Understanding how stem cells organize to form early tissue layers remains an important open question in developmental biology. Helpful understanding this process are biomarkers or features that signal when a significant transition decision occurs. We show such from the spatial layout of colony sufficient train neural networks classify cell colonies according differentiation protocol treatments each has received. use topological data analysis derive input information about cells' positions...
Understanding the multicellular organization of stem cells is vital for determining mechanisms that coordinate cell fate decision-making during differentiation; these range from neighbor-to-neighbor communication to tissue-level biochemical gradients. Current methods quantifying patterning tend capture spatial properties colonies at a fixed scale and typically rely on human annotation. We present computational pipeline utilizes topological data analysis generate quantitative, multiscale...
Although doxorubicin toxicity in cancer cells is multifactorial, the enzymatic bioactivation of drug can significantly contribute to its cytotoxicity. Previous research has identified most components that comprise network; however, adaptation network changes treatment or patient-specific much less understood. To investigate properties coupled reduction/oxidation reactions network, we analyzed metabolic differences between two patient-derived acute lymphoblastic leukemia (ALL) cell lines...
Clinical debate has arisen over the consequences of antioxidant supplementation during cancer chemotherapy. While antioxidants may impede efficacy chemotherapy by scavenging reactive oxygen species and free radicals, it is also possible that alleviate unwanted chemotherapy-induced toxicity, thus allowing for increased doses. These contradictory assertions suggest treatment can have varied outcomes depending on cellular context. To gain a more robust understanding role play in chemotherapy,...
In this report we present a new chemical probe, 3-HTC, that can reversibly and ratiometrically measure the thiol-disulfide equilibrium of biological systems. 3-HTC is composed coumarin has thiolate directly conjugated to its extended aromatic π system while formation disulfide attenuates conjugation. The fluorescence absorption properties are therefore very sensitive redox state thiol. reacts with thiols disulfides enabling use dynamic GSH/GSSH ratios in vitro as well monitor reversible...
Inflammation plays a central role in atherosclerosis. However, the detailed changes composition and quantity of leukocytes arterial wall during atherogenesis are not fully understood part because lack suitable methods animal models.We developed 10-fluorochrome, 13-parameter flow cytometry method to quantitate 7 major leukocyte subsets single digested sample. Apolipoprotein E-deficient mice underwent left carotid artery (LCA) partial ligation were fed high-fat diet for 4 28 days....
Microfluidic platform for generating dynamic profile of cell stimuli non-adherent cells.
The initiation of heterogeneity within a population phenotypically identical progenitors is critical event for the onset morphogenesis and differentiation patterning. Gap junction communication multicellular systems produces complex networks intercellular connectivity that result in heterogeneous distributions intracellular signaling molecules. In this study, we investigate emergent systems-level behavior network embryonic stem cell (ESC) populations corresponding spatial organization during...
Abstract CRISPR/Cas9 systems have been used in a wide variety of biological studies; however, the large size presents challenges packaging it within adeno-associated viruses (AAVs) for clinical applications. We identified two-cassette system expressing pieces S. pyogenes Cas9 (SpCas9) protein which splice together cellula to form functional capable site-specific DNA cleavage. With specific CRISPR guide strands, we demonstrated efficacy this cleaving HBB and CCR5 genes human HEK-293T cells as...
Titanium dioxide (TiO2) nanoparticles, used as pigments and photocatalysts, are widely present in modern society. Inhalation or ingestion of these nanoparticles can lead to cellular-level interactions. We examined the very first step this cellular interaction, effect TiO2 on lipids plasma membrane. Within 12 h nanoparticle exposure, membrane were oxidized, determined with a malondialdehyde assay. Lipid peroxidation was inhibited by surface passivation incubation an antioxidant (Trolox),...
Pluripotent embryonic stem cells (ESCs) have the unique ability to differentiate into from all germ lineages, making them a potentially robust cell source for regenerative medicine therapies, but difficulties in predicting and controlling ESC differentiation currently limit development of therapies applications such cells. A common approach induce ESCs vitro is via formation multicellular aggregates known as embryoid bodies (EBs), yet fate specification within EBs generally considered an...