A5085193722- Mesenchymal stem cell research
- Bone fractures and treatments
- Wound Healing and Treatments
- Cancer Cells and Metastasis
- Bone Tissue Engineering Materials
- Biomedical Ethics and Regulation
- Pluripotent Stem Cells Research
- Orthopedic Surgery and Rehabilitation
- Spine and Intervertebral Disc Pathology
- Hematopoietic Stem Cell Transplantation
- Craniofacial Disorders and Treatments
- Surgical Sutures and Adhesives
- Periodontal Regeneration and Treatments
- Immune cells in cancer
- Dental Implant Techniques and Outcomes
- Diabetic Foot Ulcer Assessment and Management
- Antibiotic Resistance in Bacteria
- Cleft Lip and Palate Research
- Medical Imaging and Analysis
- Abdominal Trauma and Injuries
- Dermatologic Treatments and Research
- Reconstructive Surgery and Microvascular Techniques
- Osteoarthritis Treatment and Mechanisms
- FOXO transcription factor regulation
- Infective Endocarditis Diagnosis and Management
Stanford University
1993-2024
University of Manchester
2015-2023
Mayo Clinic
2012-2022
Lehigh Valley Health Network
2022
Manchester Academic Health Science Centre
2022
Blond McIndoe Research Foundation
2021
Manchester University NHS Foundation Trust
2021
Institute for Stem Cell Biology and Regenerative Medicine
2021
Haemonetics (United States)
2020
Mayo Clinic in Arizona
2020
Skeletal stem and progenitor cell populations are crucial for bone physiology. Characterization of these types remains restricted to heterogenous bulk with limited information on whether they unique or overlap previously characterized types. Here we show, through comprehensive functional single-cell transcriptomic analyses, that postnatal long bones mice contain at least two progenitors bona fide skeletal (SSC) characteristics. An early osteochondral SSC (ocSSC) facilitates growth repair,...
Sexually dimorphic tissues are formed by cells that regulated sex hormones. While a number of systemic hormones and transcription factors known to regulate proliferation differentiation osteoblasts osteoclasts, the mechanisms determine sexually differences in bone regeneration unclear. To explore how regeneration, we compared fracture repair between adult male female mice. We found skeletal stem cell (SSC) mediated mice is dependent on estrogen signaling but SSCs from do not exhibit similar...
Glucocorticoid (GC) induced osteoporosis (GIOP) and osteonecrosis remain a significant health issue with few approved therapies that can treat the bone loss dysfunction of skeletal vasculature. Therefore, we aimed to investigate cellular molecular processes by which GCs affect osteogenesis angiogenesis, as well how treatment parathyroid hormone (hPTH 1-34) modifies these effects in mouse model GIOP. GC reduced mass through decreased formation stem cells (SSCs) while also increasing...
GABRR1 is a rho subunit receptor of GABA, the major inhibitory neurotransmitter in mammalian brain. While most investigations its function focused on nervous system, regulatory role hematopoiesis has not been reported. In this study, we found mainly expressed subsets human and mouse hematopoietic stem cells (HSCs) megakaryocyte progenitors (MkPs). GABRR1-negative (GR-) HSCs led to higher donor-derived chimerism than GABRR1-positive (GR+) HSCs. GR+ but GR- MkPs respond GABA patch clamp...
Objective: To investigate the effects of local doxycycline administration on skin scarring. Background: Skin scarring represents a major source morbidity for surgical patients. Doxycycline, tetracycline antibiotic with off-target extracellular matrix, has demonstrated antifibrotic in multiple organs. However, doxycycline's potential have not been explored vivo. Methods: Female C57BL/6J mice underwent dorsal wounding following an established splinted excisional model. Doxycycline was...
Ex situ atomic force microscopy and x-ray photoelectron spectroscopy are employed to characterise the adsorption of calcium phosphate from an aqueous solution CaCl2.H2O KH2PO4 onto rutile-TiO2(110) α-Al2O3(0001). Prior immersion, substrates underwent wet chemical preparation produce well-defined surfaces. Calcium is observed on both α-Al2O3(0001), with images indicating island-type growth. In contrast other studies less TiO2 Al2O3 substrates, induction period for nucleation appears be...
Abstract Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging delivery 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction CreER T2 recombinase in mice. Our method permits precise, vivo, tissue-specific clonal analysis with both spatial temporal control. This technology is effective using mice specific ubiquitous Cre drivers a variety tissue types,...
The skeletal system is generated and maintained by its progenitors, stem cells (SSCs), across the duration of life. Gradual changes associated with aging result in significant differences functionality SSCs. Declines bone cartilage production, increase marrow adipose tissue, compositional cellular microenvironments, subsequent deterioration external internal structures culminate aged weakened skeleton. features mechanisms aging, progenitor particular, are topics recent investigation....
Background: The first carpometacarpal joint (CMCJ) in the hand is a commonly affected by osteoarthritis. It causes significant thumb base pain, limiting functional capacity. Microfracturing and application of autologous stem cells has been performed on large joints such as knee but never evaluated for use smaller hand. Our aim was to determine potential benefit microfracturing bone marrow treatment osteoarthritis CMCJ Methods: All inclusion criteria were satisfied. Preoperative assessment...