A5085896902- 3D Printing in Biomedical Research
- Nanofabrication and Lithography Techniques
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Osteoarthritis Treatment and Mechanisms
- Cellular Mechanics and Interactions
- Additive Manufacturing and 3D Printing Technologies
- Tissue Engineering and Regenerative Medicine
- Advanced Materials and Mechanics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nonlinear Optical Materials Studies
- Advanced Sensor and Energy Harvesting Materials
- Knee injuries and reconstruction techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Plasmonic and Surface Plasmon Research
- Neuroscience and Neural Engineering
- Proteoglycans and glycosaminoglycans research
- Micro and Nano Robotics
- Advanced MEMS and NEMS Technologies
- Semiconductor Lasers and Optical Devices
- Laser Material Processing Techniques
- Pluripotent Stem Cells Research
- Force Microscopy Techniques and Applications
- EEG and Brain-Computer Interfaces
- Orbital Angular Momentum in Optics
Drexel University
2015-2024
Stanford University
2011-2018
Philadelphia University
2016
The University of Texas at Austin
2005-2011
Abstract Young's modulus and Poisson's ratio of a porous polymeric construct (scaffold) quantitatively describe how it supports transmits external stresses to its surroundings. While is always non‐negative highly tunable in magnitude, can, indeed, take on negative values despite the fact that for virtually every naturally occurring artificial material. In some applications, having (an auxetic construct) may be more suitable supporting forces imposed upon by environment. Here,...
Technology of tissue-engineering advanced rapidly in the last decade and motivated numerous studies cell-engineering biofabrication. Three-dimensional (3D) scaffolds play a critical role this field, as provide biomimetic microenvironments that could stimulate desired cell behaviors for regeneration. However, despite many achievements, fabrication 3D scaffold remains challenging due to difficulty encapsulating cells scaffolds, controlling cell-cell organization 3D, being adapted by users...
This article presents a micromanufacturing method for direct projection printing of three-dimensional scaffolds applications in the field tissue engineering by using digital micromirror-array device (DMD) layer-by-layer process. Multilayered are microfabricated curable materials through an ultraviolet (UV) photopolymerization The prepatterned UV light is projected onto photocurable polymer solution creating “photomask” design with graphic software. Poly(ethylene glycol) diacrylate mixed...
Adipose-derived stromal cells (ADSCs) are attractive autologous cell sources for cartilage repair given their relative abundance and ease of isolation. Previous studies have demonstrated the potential extracellular matrix (ECM) molecules as three-dimensional (3D) scaffolds promoting chondrogenesis. However, few compared effects varying types or doses ECM on chondrogenesis ADSCs in 3D. Furthermore, increasing molecule concentrations often result simultaneous changes stiffness, which makes it...
Abstract Hydrogel‐based scaffolds are widely used for culturing cells in three dimensions due to their tissue‐like water content and tunable biochemical physical properties. Most conventional hydrogels lack the macroporosity desirable efficient cell proliferation migration have limited flexibility when subject mechanical load. Here microribbon‐like elastomers that, photocrosslinked, can form macroporous highly flexible that support 3D developed. These microribbons produced by wet‐spinning...
Hydrogels are widely used as three-dimensional (3D) tissue engineering scaffolds due to their tissue-like water content, well tunable physical and chemical properties. Hydrogel-based generally associated with nanoscale porosity, whereas macroporosity is highly desirable facilitate nutrient transfer, vascularization, cell proliferation matrix deposition. Diverse techniques have been developed for introducing into hydrogel-based scaffolds. However, most of these methods involve harsh...
To elucidate the role of decorin, a small leucine-rich proteoglycan, in degradation cartilage matrix during progression post-traumatic osteoarthritis (OA).Three-month-old decorin-null (Dcn-/- ) and inducible decorin-knockout (DcniKO mice were subjected to surgical destabilization medial meniscus (DMM) induce OA. The OA phenotype that resulted was evaluated by assessing joint morphology sulfated glycosaminoglycan (sGAG) staining via histological analysis (n = 6 per group), surface collagen...
Hydrogels (HGs) are attractive matrices for cell-based cartilage tissue regeneration given their injectability and ability to fill defects with irregular shapes. However, most HGs developed date often lack cell scale macroporosity, which restrains the encapsulated cells, leading delayed new extracellular matrix deposition restricted pericellular regions. Furthermore, tissue-engineered using conventional generally suffers from poor mechanical property fails restore load-bearing of articular...
Stem cell-based therapies hold great promise for enhancing tissue regeneration. However, the majority of cells die shortly after transplantation, which greatly diminishes efficacy stem therapies. Poor cell engraftment and survival remain a major bottleneck to fully exploiting power regenerative medicine. Biomaterials such as hydrogels can serve artificial matrices protect during delivery guide desirable fates. conventional often lack macroporosity, restricts proliferation delays matrix...
ObjectivesThe objective of this study was to perform a quantitative analysis the structural and functional alterations in intervertebral disc during vivo degeneration, using emerging tools that enable rigorous assessment from microscale macroscale, as well correlate these outcomes with noninvasive, clinically relevant imaging parameters.DesignDegeneration induced rabbit model by puncturing annulus fibrosus (AF) 16-gauge needle. 2, 4, 8, 12 weeks following puncture, degenerative changes discs...
Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation Kimberly C. Dickey, Sankar Subramanian, John E. Anthony, Li-Hsin Han, Shaochen Chen, Yueh-Lin Loo; Large-area patterning of a solution-processable organic semiconductor to reduce parasitic leakage off currents in thin-film transistors. Appl. Phys. Lett. 11 June 2007; 90 (24): 244103....
Polymeric microspheres represent an injectable platform for controlling the release of a variety biologics; may be combined in modular fashion to achieve temporal two or more biomolecules. Microfluidics offers versatile synthesizing uniform polymeric harboring biologics under relatively mild conditions. Poly(ethylene glycol) (PEG) is bioinert polymer that can easily tailored encapsulate and control biologics. In this study, we report microfluidic synthesis biodegradable PEG-based...
Abstract The extracellular matrix (ECM) is a complex 3D framework of macromolecules, which regulate cell bioactivity via chemical and physical properties. ECM's properties, including stiffness constraints to shape, actomyosin cytoskeleton contractions, induce signaling cascades influencing gene expression fate. Engineering such bioactivity, a.k.a., mechanotransduction, has been mainly achieved by 2D platforms as micropatterns. These cause cytoskeletal contractions with apico‐basal polarity...
PEG-based microribbons are designed and fabricated as building blocks for constructing a 3D cell niche with independently tunable biochemical, mechanical, topographical cues. This platform supports direct encapsulation, allows spatial patterning of biochemical cues, may provide valuable tool facilitating the analyses how interactive signaling regulates fate in three dimensions. As service to our authors readers, this journal provides supporting information supplied by authors. Such materials...
Stem cells can contribute to cartilage repair either directly through chondrogenic differentiation or indirectly paracrine signaling. Using a 3D co-culture model, we have recently reported that adipose-derived stem (ADSCs) catalyze formation by neonatal chondrocytes (NChons) when mixed co-cultured in biomimetic hydrogels. However, how matrix cues influence such catalyzed remains unknown. To answer this question, ADSCs and NChons were co-encapsulated 39 combinatorial hydrogel compositions...
In this work, we demonstrate the plausibility of integrating two-photon polymerization (TPP) with nanoimprinting for direct, digital nanomanufacturing. TPP offers manufacturing nanomolds at a low cost, while process using enables massively parallel printing nanostructures. A Ti:sapphire femtosecond laser (800 nm wavelength, 100 fs pulse width, repetition rate 80 MHz) was used to induce in dipentaerythritol pentaacrylate fabricate mold 400 wide line array on glass substrate. The surface...
We report on the design, fabrication, and analysis of a light-driven micromotor. The micromotor was created from nanoporous polymer with close-packed gold nanoparticles which generate heat by absorbing light. blades were curved, forming convex concave sides. Upon lateral irradiation, geometric effect side transfers more photon-generated to surrounding gas molecules, causing convective motion leading rotation motions molecules analyzed using molecular dynamics modeling.