A5007375849- Cancer, Hypoxia, and Metabolism
- Glioma Diagnosis and Treatment
- 3D Printing in Biomedical Research
- ATP Synthase and ATPases Research
- Extracellular vesicles in disease
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Ultrasound and Hyperthermia Applications
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
- HIV/AIDS drug development and treatment
- Immune cells in cancer
- MicroRNA in disease regulation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Epigenetics and DNA Methylation
- HIV-related health complications and treatments
- Thermal Regulation in Medicine
- Asthma and respiratory diseases
- Mitochondrial Function and Pathology
- COVID-19 Clinical Research Studies
- Mathematical Biology Tumor Growth
- Pharmacy and Medical Practices
- Cancer-related Molecular Pathways
Stanford University
2019-2025
University of Arkansas for Medical Sciences
2024-2025
California Institute for Regenerative Medicine
2024
Arkansas Children's Hospital
2024
Acorn
2024
Stanford Medicine
2023
Princeton University
2017-2022
University of Wisconsin–Madison
2022
DaVita Clinical Research (United States)
2020
Farm Foundation
2016
Epithelial-mesenchymal transition (EMT) have been extensively characterized in development and cancer, its dynamics modeled as a non-linear process. However, less is known about how such may affect biological impact. Here, we use mathematical modeling experimental analysis of the TGF-β-induced EMT to reveal hysteretic response E-cadherin repression tightly controlled by strength miR-200s/ZEBs negative feedback loop. Hysteretic conveys memory state, ensures rapid robust cellular enables...
The human brain undergoes rapid development at mid-gestation from a pool of neural stem and progenitor cells (NSPCs) that give rise to the neurons, oligodendrocytes, astrocytes mature brain. Functional study these cell types has been hampered by lack precise purification methods. We describe method for prospectively isolating ten distinct NSPC developing using cell-surface markers. CD24−THY1−/lo were enriched radial glia, which robustly engrafted differentiated into all three lineages in...
The developmental origin of blood-forming hematopoietic stem cells (HSCs) is a longstanding question. Here, our non-invasive genetic lineage tracing in mouse embryos pinpoints that artery endothelial generate HSCs. Arteries are transiently competent to HSCs for 2.5 days (∼E8.5–E11) but subsequently cease, delimiting narrow time frame HSC formation vivo. Guided by the arterial origins blood, we efficiently and rapidly differentiate human pluripotent (hPSCs) into posterior primitive streak,...
Early stages of deadly respiratory diseases including COVID-19 are challenging to elucidate in humans. Here, we define cellular tropism and transcriptomic effects SARS-CoV-2 virus by productively infecting healthy human lung tissue using scRNA-seq reconstruct the transcriptional program “infection pseudotime” for individual cell types. predominantly infected activated interstitial macrophages (IMs), which can accumulate thousands viral RNA molecules, taking over 60% transcriptome forming...
There are limited data comparing the effectiveness of multidisciplinary severe asthma clinics (SACs) with that conventional single-discipline (SDCs) for pediatric asthma. Our aim was to compare outcomes between SACs and SDCs examine longitudinal health patients who were followed in SACs. We conducted a retrospective cohort study among 2018 2022 treated at Arkansas Children's SAC those SDCs. The primary outcome acute care utilization, including hospitalizations emergency department visits....
Lacrosse is a fast growing youth sport in the United States. Although there have been published studies examining injuries associated with lacrosse, significantly less research has conducted high school lacrosse players than older players. The objective of this study was to compare injury rates and patterns by type athletic activity (ie, competition vs practice) sex.Lacrosse differ sex.Descriptive epidemiology study.Using High School RIO (Reporting Information Online) database, exposure data...
Secreted proteins play crucial roles in mediating tumor-stroma interactions during metastasis of cancer to different target organs. To comprehensively profile secreted involved lung metastasis, we applied quantitative mass spectrometry-based proteomics and identified 392 breast cancer-derived 302 melanoma-derived from highly metastatic cells. The cancer-specific secretome signatures (LMSSs) displayed significant prognostic value multiple clinical data sets. Moreover, observed a overlap...
Acute kidney injury (AKI) is the abrupt loss of renal function, for which only supportive therapies exist. Mesenchymal stromal cell (MSC)-derived extracellular vesicles (EVs) have been shown to be therapeutically effective in treating AKI by spurring endogenous proliferation and survival while suppressing inflammation. Pre-treating kidneys with pulsed focused ultrasound (pFUS) has also enhance MSC therapy AKI, but its role MSC-derived EV remains unexplored. Using a mouse model...
Acute kidney injury (AKI) is characterized by a sudden failure of renal function, but despite increasing worldwide prevalence, current treatments are largely supportive, with no curative therapies. Mesenchymal stromal cell (MSC) therapy has been shown to have promising regenerative effect in AKI limited the ability cells home damaged tissue. Pulsed focused ultrasound (pFUS), wherein target tissues sonicated short bursts sound waves, reported enhance MSC homing upregulating local signals....
Diseases of the kidney contribute a significant morbidity and mortality burden on society. Localized delivery therapeutics directly into kidney, via its arterial blood supply, has potential to enhance their therapeutic efficacy while limiting side effects associated with conventional systemic delivery. Targeted in humans is feasible given that we can access renal supply using minimally invasive endovascular techniques imaging guidance. However, there currently no described way reproduce or...
Abstract Background Acute kidney injury (AKI) is characterized by rapid failure of renal function and has no curative therapies. Mesenchymal stromal cell (MSC)-derived extracellular vesicles (EVs) are known to carry therapeutic factors, which have shown promise in regenerative medicine applications, including AKI. However, there remains an unmet need optimize their effect. One potential avenue optimization lies pulsed focused ultrasound (pFUS), where tissues-of-interest treated with sound...
ABSTRACT Early stages of deadly respiratory diseases such as COVID-19 have been challenging to elucidate due lack an experimental system that recapitulates the cellular and structural complexity human lung while allowing precise control over disease initiation systematic interrogation molecular events at resolution. Here we show healthy slices cultured ex vivo can be productively infected with SARS-CoV-2, tropism virus its distinct dynamic effects on host cell gene expression determined by...
Pyruvate kinase M2 (PKM2) catalyzes the final step in glycolysis, a key process of cancer metabolism. PKM2 is preferentially expressed by glioblastoma (GBM) cells with minimal expression healthy brain. We describe development, validation, and translation novel PET tracer to study GBM. evaluated 1-((2-fluoro-6-[
The effect of Menoprogen (MPG) on ovarian granulosa cell (GC) apoptosis was investigated in vitro and vivo an aged rat model menopause. Intragastric administration or estradiol valerate to 14-month-old senile female rats for eight weeks increased plasma<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">E</mml:mi></mml:mrow><mml:mrow><mml:mn...