A5035544100- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Immune cells in cancer
- Radiopharmaceutical Chemistry and Applications
- Zebrafish Biomedical Research Applications
- bioluminescence and chemiluminescence research
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
California Institute for Regenerative Medicine
2023-2024
Stanford University
2022-2024
The University of Tokyo
2022
The developmental origin of blood-forming hematopoietic stem cells (HSCs) is a longstanding question. Here, our non-invasive genetic lineage tracing in mouse embryos pinpoints that artery endothelial generate HSCs. Arteries are transiently competent to HSCs for 2.5 days (∼E8.5–E11) but subsequently cease, delimiting narrow time frame HSC formation vivo. Guided by the arterial origins blood, we efficiently and rapidly differentiate human pluripotent (hPSCs) into posterior primitive streak,...
Hematopoietic stem cells (HSCs) are a rare type of hematopoietic cell that can entirely reconstitute the blood and immune system after transplantation. Allogeneic HSC transplantation (HSCT) is used clinically as curative therapy for range hematolymphoid diseases; however, it remains high-risk because its potential side effects, including poor graft function graft-versus-host disease (GVHD). Ex vivo expansion has been suggested an approach to improve reconstitution in low-cell dose grafts....
A long-sought goal of cancer immunotherapy is to mass-produce T cells that specifically target tumor neoantigens. One decisive challenge the identification neoantigens derived from driver genes. Here, we identify recognize NSCLC-associated EGFR C797S mutation, which confers resistance current inhibitors and linked poor prognosis. To overcome limitations in cell availability, reprogrammed C797S-specific into induced pluripotent stem (iPSCs) re-differentiated them CD8⁺ cells. These...