A5087335190- Lung Cancer Treatments and Mutations
- Lung Cancer Diagnosis and Treatment
- RNA modifications and cancer
- Pleural and Pulmonary Diseases
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Myasthenia Gravis and Thymoma
- Cancer, Hypoxia, and Metabolism
- Medical Imaging and Pathology Studies
- Respiratory Support and Mechanisms
- Radiomics and Machine Learning in Medical Imaging
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Lung Cancer Research Studies
- Esophageal Cancer Research and Treatment
- Epigenetics and DNA Methylation
- Gastric Cancer Management and Outcomes
- Glycosylation and Glycoproteins Research
- Esophageal and GI Pathology
- Muscle Physiology and Disorders
- Ferroptosis and cancer prognosis
- Pituitary Gland Disorders and Treatments
- Tracheal and airway disorders
- Congenital Diaphragmatic Hernia Studies
- Airway Management and Intubation Techniques
- Trauma Management and Diagnosis
VA Palo Alto Health Care System
2016-2025
Stanford University
2016-2025
Stanford Medicine
2011-2024
Stanford Health Care
2009-2024
Memorial Sloan Kettering Cancer Center
2024
Cardiovascular Institute of the South
2023
Cancer Prevention Institute of California
2017-2021
Stanford Cancer Institute
2013-2021
Central University of Ecuador
2021
Mayo Clinic in Florida
2021
The protein dystrophin, normally found on the cytoplasmic surface of skeletal muscle cell membranes, is absent in patients with Duchenne muscular dystrophy as well mdx (X-linked dystrophy) mice. Although its primary structure has been determined, precise functional role dystrophin remains subject speculation. In present study, we demonstrate that dystrophin-deficient fibers mouse exhibit an increased susceptibility to contraction-induced sarcolemmal rupture. level damage directly correlated...
The combination of complete diaphragm inactivity and mechanical ventilation (for more than 18 hours) elicits disuse atrophy myofibers in animals. We hypothesized that the same may also occur human diaphragm.
Identifying molecular residual disease (MRD) after treatment of localized lung cancer could facilitate early intervention and personalization adjuvant therapies. Here, we apply personalized profiling by deep sequencing (CAPP-seq) circulating tumor DNA (ctDNA) analysis to 255 samples from 40 patients treated with curative intent for stage I-III 54 healthy adults. In 94% evaluable experiencing recurrence, ctDNA was detectable in the first posttreatment blood sample, indicating reliable...
Abstract Volumetric muscle loss (VML) is associated with of skeletal function, and current treatments show limited efficacy. Here we that bioconstructs suffused genetically-labelled stem cells (MuSCs) other resident (MRCs) are effective to treat VML injuries in mice. Imaging implanted damaged muscles indicates MuSCs survival growth, ex vivo analyses force restoration treated muscles. Histological analysis highlights myofibre formation, neovascularisation, but insufficient innervation. Both...
Abstract Medical image biomarkers of cancer promise improvements in patient care through advances precision medicine. Compared to genomic biomarkers, provide the advantages being non-invasive, and characterizing a heterogeneous tumor its entirety, as opposed limited tissue available via biopsy. We developed unique radiogenomic dataset from Non-Small Cell Lung Cancer (NSCLC) cohort 211 subjects. The comprises Computed Tomography (CT), Positron Emission (PET)/CT images, semantic annotations...
Abstract Elucidating the spectrum of epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial (MET) states in clinical samples promises insights on cancer progression drug resistance. Using mass cytometry time-course analysis, we resolve lung EMT through TGFβ-treatment identify, TGFβ-withdrawal, a distinct MET state. We demonstrate significant differences between trajectories using computational tool (TRACER) for reconstructing cell states. In addition, construct reference map...
Adult skeletal muscle stem cells, or satellite cells (SCs), regenerate functional following transplantation into injured diseased tissue. To gain insight human SC (huSC) biology, we analyzed transcriptome dynamics by RNA sequencing of prospectively isolated quiescent and activated huSCs. This analysis indicated that huSCs differentiate lose proliferative potential when maintained in high-mitogen conditions ex vivo. Further gene expression revealed p38 MAPK acts a transcriptional network...
<h3>Importance</h3> Guidelines recommend using gabapentin to decrease postoperative pain and opioid use, but significant variation exists in clinical practice. <h3>Objective</h3> To determine the effect of perioperative on remote time resolution cessation. <h3>Design, Setting, Participants</h3> A randomized, double-blind, placebo-controlled trial was conducted at a single-center, tertiary referral teaching hospital. total 1805 patients aged 18 75 years scheduled for surgery (thoracotomy,...
To identify disease-relevant T cell receptors (TCRs) with shared antigen specificity, we analyzed 778,938 TCRβ chain sequences from 178 non-small lung cancer patients using the GLIPH2 (grouping of lymphocyte interactions paratope hotspots 2) algorithm. We identified over 66,000 specificity groups, which 435 were clonally expanded and enriched in tumors compared to adjacent lung. The antigenic epitopes one such tumor-enriched group a yeast peptide-HLA A∗02:01 display library. These included...
Early stages of deadly respiratory diseases including COVID-19 are challenging to elucidate in humans. Here, we define cellular tropism and transcriptomic effects SARS-CoV-2 virus by productively infecting healthy human lung tissue using scRNA-seq reconstruct the transcriptional program “infection pseudotime” for individual cell types. predominantly infected activated interstitial macrophages (IMs), which can accumulate thousands viral RNA molecules, taking over 60% transcriptome forming...
Purpose To create a radiogenomic map linking computed tomographic (CT) image features and gene expression profiles generated by RNA sequencing for patients with non–small cell lung cancer (NSCLC). Materials Methods A cohort of 113 NSCLC diagnosed between April 2008 September 2014 who had preoperative CT data tumor tissue available was studied. For each tumor, thoracic radiologist recorded 87 semantic features, selected to reflect radiologic characteristics nodule shape, margin, texture,...
Abstract Molecular analysis of the mutation status for EGFR and KRAS are now routine in management non-small cell lung cancer. Radiogenomics, linking medical images with genomic properties human tumors, provides exciting opportunities non-invasive diagnostics prognostics. We investigated whether can be predicted using imaging data. To accomplish this, we studied 186 cases NSCLC preoperative thin-slice CT scans. A thoracic radiologist annotated 89 semantic image features each patient’s tumor....
Rationale: Patients on mechanical ventilation who exhibit diaphragm inactivity for a prolonged time (case subjects) develop decreases in force-generating capacity accompanied by myofiber atrophy.Objectives: Our objectives were to test the hypotheses that increased proteolysis ubiquitin-proteasome pathway, myosin heavy chain (MyHC) levels, and atrophic AKT-FOXO signaling play major roles eliciting these pathological changes associated with disuse.Methods: Biopsy specimens obtained from costal...
The present project involved a collective effort agreed by the European Society of Thoracic Surgeons, American Association for Surgery, and General Surgery Club to assemble joint panel experts review available data address ambiguous aspects chest tube definitions nomenclature. task force was composed 11 invited participants, identified their expertise in area management. subject divided different topics, which were turn assigned at least two experts. draft reports written on each topic...
Aging leads to skeletal muscle atrophy (i.e., sarcopenia), and fiber loss is a critical component of this process. The mechanisms underlying these age-related changes, however, remain unclear. We show here that mTORC1 signaling activated in subset fibers aging mouse human, colocalized with damage. Activation TSC1 knockout increases the content morphologically abnormal mitochondria causes progressive oxidative stress, damage, over lifespan. Transcriptomic profiling reveals mTORC1's activation...