A5100333740- Immune cells in cancer
- Osteoarthritis Treatment and Mechanisms
- Immune Cell Function and Interaction
- Genetic Mapping and Diversity in Plants and Animals
- Bone Metabolism and Diseases
- Nutrition and Health in Aging
- CAR-T cell therapy research
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Immune Response and Inflammation
- Bone health and treatments
- Rice Cultivation and Yield Improvement
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- NF-κB Signaling Pathways
- Yersinia bacterium, plague, ectoparasites research
- Muscle Physiology and Disorders
- Wnt/β-catenin signaling in development and cancer
- Cancer-related Molecular Pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- Mast cells and histamine
- Alzheimer's disease research and treatments
- Extracellular vesicles in disease
- PI3K/AKT/mTOR signaling in cancer
First Hospital of Jilin University
2019-2025
Jilin University
2019-2025
Xiangya Hospital Central South University
2025
Central South University
2021-2025
Hunan Cancer Hospital
2025
Shanghai Jiao Tong University
2011-2025
Sun Yat-sen University
2024-2025
Zhejiang University
2025
Third Affiliated Hospital of Sun Yat-sen University
2025
Anhui University of Science and Technology
2019-2025
Cell‐membrane‐coated nanoparticles have recently been studied extensively for their biological compatibility, retention of cellular properties, and adaptability to a variety therapeutic imaging applications. This class nanoparticles, which has fabricated with cell membrane coatings, including those derived from red blood cells (RBCs), platelets, white cells, cancer bacteria, exhibit properties that are characteristic the source cell. In this study, new type coating is created by fusing...
Objectives Mammalian target of rapamycin (mTOR) (a serine/threonine protein kinase) is a major repressor autophagy, cell survival mechanism. The specific in vivo mechanism mTOR signalling OA pathophysiology not fully characterised. We determined the expression and known autophagy genes human cartilage as well mouse dog models experimental OA. created cartilage-specific knockout (KO) mice to determine role vivo. Methods Inducible KO were generated subjected model Human chondrocytes treated...
Objectives To investigate the roles and regulatory mechanisms of synovial macrophages their polarisation in development osteoarthritis (OA). Methods Synovial tissues from normal patients with OA were collected. M1 or M2-polarised mice analysed by immunofluorescence immunohistochemical staining. Mice tuberous sclerosis complex 1 (TSC1) Rheb deletion specifically myeloid lineage generated subjected to intra-articular injection collagenase (collagenase-induced osteoarthritis, CIOA)...
Protein poly(ADP-ribosyl)ation and ubiquitination are two key post-translational modifications regulating many biological processes. Through crystallographic biochemical analysis, we show that the RNF146 WWE domain recognizes poly(ADP-ribose) (PAR) by interacting with iso -ADP-ribose ( -ADPR), smallest internal PAR structural unit containing characteristic ribose–ribose glycosidic bond formed during poly(ADP-ribosyl)ation. The -ADPR-binding residues identified highly conserved among domains....
<h3>Objectives</h3> We have previously shown that peroxisome proliferator-activated receptor gamma (PPARγ), a transcription factor, is essential for the normal growth and development of cartilage. In present study, we created inducible cartilage-specific PPARγ knockout (KO) mice subjected these to destabilisation medial meniscus (DMM) model osteoarthritis (OA) elucidate specific in vivo role OA pathophysiology. further investigated downstream signalling pathway responsible maintaining...
The role of tumor-resident microbiota in modulating tumor immunity remains unclear. Here, we discovered an abundance intra-tumoral bacteria, such us E.coli, residing and resulting Colorectal cancer liver metastasis (CRLM). E.coli enhanced lactate production, which mediated M2 macrophage polarization by suppressing nuclear factor-κB -gene binding (NF-κB) signaling through retinoic acid-inducible gene 1 (RIG-I) lactylation. Lactylation RIG-I suppressed recruitment NF-κB to the Nlrp3 promoter...
Emerging evidence suggests that connexin mediated gap junctional intercellular communication contributes to many aspects of bone biology including development, maintenance homeostasis and responsiveness cells diverse extracellular signals. Deletion 43, the predominant junction protein in bone, is embryonic lethal making it challenging examine role 43 vivo. However, transgenic murine models which only osteocytes osteoblasts are deficient fully viable, have recently been developed....
Abstract Exosomes are small membrane‐bound vesicles released into extracellular spaces by many types of cells. These nanovesicles carry proteins, mRNA, and miRNA, involved in cell waste management intercellular communication. In the present study, it is shown that exosome release, which leads to net loss cellular membrane protein content, negatively regulated mechanistic target rapamycin complex 1 (mTORC1). It found cells animal models release inhibited sustained activation mTORC1, leading...
The tumor microenvironment is composed of cells, fibroblasts, endothelial cells and infiltrating immune which may inhibit or promote growth progression. objectives this retrospective study were to characterize the density tumor-associated macrophages (TAMs) in breast cancer, correlate TAMs with clinicopathological parameters. Paraffin-embedded specimens data, including up 5 years follow-up information, obtained from 172 cancer patients. Immunohistochemical staining for CD68 (marker...
Myeloid‐derived suppressor cells ( MDSC s) accumulate in tumor‐bearing hosts and play a major role tumor‐induced immunosuppression, which hampers effective immuno‐therapeutic approaches. β‐Glucans have been reported to function as potent immuno‐modulators stimulate innate adaptive immune responses, contributes their antitumor property. Here, we investigated the effect of particulate β‐glucans on s found that β‐glucan treatment could promote differentiation M ‐ (monocytic into more mature CD...
We examined the structural plasticity of excitatory synapses from corticostriatal and thalamostriatal pathways their postsynaptic targets in adult Sprague-Dawley rats to understand how these striatal circuits change l-DOPA-induced dyskinesias (LIDs). present here detailed electron light microscopic analyses that provide new insight into nature synaptic remodeling medium spiny neurons response LIDs. Numerous studies have implicated enhanced glutamate signaling persistent long-term...
ABSTRACT Mechanical unloading induces muscle atrophy and bone loss; however, the time course interdependence of these effects is not well defined. We subjected 4-month-old C57BL/6J mice to hindlimb suspension (HLS) for 3 weeks, euthanizing 12 16 on day (D) 0, 7, 14, 21. Lean mass was 7% 9% lower HLS versus control from D7–21. Absolute gastrocnemius (gastroc) decreased 8% by D7, maximally 16% D14 HLS. mRNA levels Atrogin-1 in gastroc quadriceps (quad) were increased 99% 122%, respectively, at...
Abstract Connexin 43 (Cx43) is the most abundant gap junction protein in bone and has been demonstrated as an integral component of skeletal homeostasis. In present study, we sought to further refine role Cx43 response mechanical unloading by subjecting skeletally mature mice with a bone-specific deletion (cKO) 3 weeks via hindlimb suspension (HLS). The HLS model was selected recapitulate effects due prolonged bed rest, reduced activity associated aging, spaceflight microgravity. At...
HOXA transcript antisense RNA myeloid-specific 1 (HOTAIRM1) is a long non-coding that has been shown to be key regulator of myeloid cell development by targeting HOXA1. Myeloid-derived suppressor cells (MDSCs) are heterogeneous population immature possess immunosuppressive function. However, the impact HOTAIRM1 on MDSCs remains unknown. In this study, we demonstrated was expressed in and over-expression could down-regulate expression suppressive molecules MDSCs. addition, levels were...
Stroke is accompanied by a distinguished inflammatory reaction that initiated the infiltration of immunocytes, expression cytokines, and other mediators. As natural killer cells (NK cells) are type cytotoxic lymphocyte critical to innate immune system, we investigated mechanism NK cells-induced brain injuries after cerebral ischemia chemotactic effect IP-10 simultaneously.NK infiltration, interferon-gamma (IFN-γ) were detected immunohistochemistry, immunofluorescence, PCR flow cytometry in...
Abstract Clinical evidence has established that concomitant traumatic brain injury (TBI) accelerates bone healing, but the underlying mechanism is unclear. This study shows after TBI, injured neurons, mainly those in hippocampus, release osteogenic microRNA (miRNA)-enriched small extracellular vesicles (sEVs), which targeted osteoprogenitors to stimulate formation. We show miR-328a-3p and miR-150-5p, enriched sEVs promote osteogenesis by directly targeting 3′UTR of FOXO4 or CBL ,...
Chronic kidney disease (CKD) is highly prevalent worldwide, and its global burden substantial growing. CKD displays a number of features accelerated senescence. Tubular cell senescence common biological process that contributes to progression. Tubulointerstitial inflammation driver tubular characteristic CKD. However, the mechanism by which interstitial drives remains unclear. This paper aims explore role exosomal miRNAs derived from macrophages in development
This study aimed to investigate the association of cystatin C, serum creatinine and sarcopenia index with cardiovascular all-cause death in general population.
A type III secretion system (T3SS) in pathogenic Yersinia species functions to translocate Yop effectors, which modulate cytokine production and regulate cell death macrophages. Distinct pathways of T3SS-dependent caspase-1 activation occur Yersinia-infected One pathway macrophages requires the effector YopJ. YopJ is an acetyltransferase that inactivates MAPK kinases IKKβ cause TLR4-dependent apoptosis naïve isoform Y. pestis KIM (YopJKIM) has two amino acid substitutions, F177L K206E, not...
Abstract Myeloid-derived suppressor cells (MDSCs) play a critical role in tumor-associated immunosuppression, thus affecting effective immunotherapies for cancers. However, the molecular mechanisms involved regulating differentiation and function of MDSCs remain largely unclear. In this study, we found that inhibition microRNA (miR)-9 promoted with significantly reduced immunosuppressive whereas overexpression miR-9 markedly enhanced MDSCs. Notably, knockdown impaired activity inhibited...
Accumulating evidence has confirmed that malignant tumors have a complex microenvironment, which consists of heterogeneous collection tumor cells and other cell subsets (including the full gamut immune cells). Tumor-associated macrophages (TAMs), derived from circulating Ly6Chi monocytes, constitute most substantial fraction tumor-infiltrating in nearly all cancer types contribute to progression, vascularization, metastasis, immunosuppression, therapeutic resistance. Interrupting monocyte...
The mechanistic target of rapamycin complex 1 (mTORC1) is a critical sensor for bone homeostasis and formation; however, the role mTORC1 in osteoclast development underlying mechanisms have not yet been fully established. Here, we found that activity declined during precursors differentiation vitro vivo. We further targeted deletion Raptor (mTORC1 key component) or Tsc1 negative regulator) to constitutively inhibit activate (monocytes/macrophages), using LyzM-cre mice. Osteoclastic formation...
Myeloid-derived suppressor cells (MDSCs) have strong immunosuppressive functions and contribute to the formation of tumor microenvironment. Long non-coding (Lnc) RNAs are highly important factors associated with tumors may be used as markers for diagnosis, which is valuable targeted therapy. LncRNA MALAT1 expressed in various tissues plays a critical role cell proliferation, including tumorigenesis metastasis. However, MDSCs unclear. In this study, we observed an increased proportion...