A5080253242- NF-κB Signaling Pathways
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Immune Response and Inflammation
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Multiple Myeloma Research and Treatments
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- DNA Repair Mechanisms
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- interferon and immune responses
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Chronic Lymphocytic Leukemia Research
- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
Stony Brook University
2010-2022
Gundersen Health System
2022
Biomedical Research Foundation of the Academy of Athens
2017-2021
Academy of Athens
2018-2021
University of Ioannina
2012-2019
San Diego State University
2018-2019
Istituto Ortopedico Rizzoli
2009-2013
Stony Brook University Hospital
2010-2012
FORTH Institute of Molecular Biology and Biotechnology
2012
University of Bologna
2005-2010
High serum levels of IL-6 correlate with poor outcome in breast cancer patients. However, no data are available on the relationship between and mammary stem/progenitor cells, which may fuel genesis vivo. Herein, we address this issue MCF-7 cell line primary human mammospheres (MS), multicellular structures enriched cells gland. MS from node invasive carcinoma tissues expressed mRNA at higher than did matched non-neoplastic glands. In addition, was detected only basal-like tissues, an...
A crude extract of commercial wheat germ is capable translating mRNAs from widely different sources with high efficiencies. Of six germs analyzed only one was found a level or incorporation natural mRNAs. Under optimum conditions at saturating Tobacco Mosaic Virus (TMV) RNA (4.5 μg) and labeled amino acid, 68% all the available 14C leucine incorporated in 70 min. 30°C stimulation 425 fold above background (with an efficiency 252 moles leucine/mole TMV RNA). Thus this system which 30 more...
ME1a1, a 16-base-pair nuclear factor binding site residing between the c-MYC P1 and P2 transcription initiation sites, is required for activity. A cDNA encoding 477-amino acid zinc finger protein designated MAZ (MYC-associated protein) was cloned from HeLa lambda gt11 library by screening with concatamerized ME1a1 probe. In addition to six potential fingers of Cys2His2 type, contains an amino-terminal proline-rich domain several polyalanine tracts. Its mRNA present in all human tissues...
Tissue damage is usually followed by healing, as both differentiated and stem cells migrate to replace dead or damaged cells. Mesoangioblasts (vessel-associated that can repair muscles) fibroblasts toward soluble factors released tissue. Two such are high mobility group box 1 (HMGB1), a nuclear protein undergoing unscheduled death (necrosis) but not apoptotic cells, stromal derived factor (SDF)–1/CXCL12. We find HMGB1 activates the canonical κB (NF-κB) pathway via extracellular...
NIARD (non-immunoglobulin-associated rearranging DNA) is located on mouse chromosome 15 at the break point of a commonly observed translocation event involving chromosomes and 12 in murine plasmacytomas. The human cellular analogue v- myc oncogene avian myelocytomatosis virus, strain MC-29, known to reside distal end 8 has been translocate 14 Burkitt lymphomas. Using cDNA clone specific for transcript c- gene (H ), we show that (M ) contained within NIARD. NIARD-associated translocations...
Tax corresponds to a 40-kDa transforming protein from the pathogenic retrovirus human T-cell leukemia virus type 1 (HTLV-1) that activates nuclear expression of NF-κB/Rel family transcription factors by an unknown mechanism. promotes N-terminal phosphorylation and degradation IκBα, principal cytoplasmic inhibitor NF-κB. Our studies now demonstrate HTLV-1 recently identified cellular kinases IκB kinase α (IKKα) IKKβ, which normally phosphorylate IκBα on both its regulatory serines in response...
The IKKβ and NEMO/IKKγ subunits of the NF-κB-activating signalsome complex are known to be essential for activating NF-κB by inflammatory other stress-like stimuli. However, IKKα subunit is believed dispensable latter responses instead functions as an <i>in vivo</i>mediator novel NF-κB-dependent -independent functions. In contrast this generally accepted view IKKα's physiological functions, we demonstrate in mouse embryonic fibroblasts (MEFs) that, akin NEMO/IKKγ, also a global regulator...
Extracellular and intracellular mediators of inflammation, such as tumor necrosis factor alpha (TNFα) NF-kappaB (NF-κB), play major roles in breast cancer pathogenesis, progression relapse. SLUG, a mediator the epithelial-mesenchymal transition process, is over-expressed CD44(+)/CD24(-) initiating cells basal-like carcinoma, subtype aggressive endowed with stem cell-like gene expression profile. Cancer also over-express members pro-inflammatory NF-κB network, but their functional...
The IκB kinase (IKK) signaling complex is responsible for activating NF-κB-dependent gene expression programs. Even though NF-κB-responsive genes are known to orchestrate stress-like responses, critical gaps in our knowledge remain about the global effects of NF-κB activation on cellular physiology. DNA microarrays were used compare programs a model system 70Z/3 murine pre-B cellsversus their IKK signaling-defective 1.3E2 variant with lipopolysaccharide (LPS), interleukin-1 (IL-1), or...
NF-B corresponds to an inducible eukaryotic transcription factor complex that is negatively regulated in resting cells by its physical assembly with a family of cytoplasmic ankyrin-rich inhibitors termed IB.Stimulation various proinflammatory cytokines, including tumor necrosis alpha (TNF-␣), induces nuclear expression.TNF-␣ signaling involves the recruitment at least three proteins (TRADD, RIP, and TRAF2) type 1 TNF-␣ receptor tail, leading sequential activation downstream NF-B-inducing...
Abstract Macrophages respond to infection with pathogenic Yersinia species by activating MAPK- and NF-κB-signaling pathways. To counteract this response, Yersiniae secrete a protease (Yersinia outer protein J (YopJ)) that is delivered into macrophages, deactivates pathways, induces apoptosis. NF-κB promotes cell survival up-regulating expression of several apoptosis inhibitor genes. Previous studies show deactivation the pathway YopJ important for Yersinia-induced determine whether...
The complete nucleotide sequence of the γ2b constant region gene cloned from BALB/c liver DNA is reported. approximately 1870 base pairs includes 5′ flanking, 3′ untranslated, and flanking regions three introns. Cγ2b coding divided by these introns into four segments corresponding to homology domains hinge protein. separating C H 2 domain 3 are small (106 119 pairs). A larger intervening 314 separates 1 regions. stretch comprising this large intron plus shows a strong with other domains.
During B lymphocyte differentiation, switches in the expression of heavy chain immunoglobulin constant region (C H ) genes occur by a novel DNA recombination mechanism. We have investigated requirements C gene switch characterizing two rearranged γ 2b from producing mouse myeloma (MPC-11). One is present 2-3 copies per cell ( strong hybridizer) while other ˜1 copy weak hybridizer). Genomic clones strongly hybridizing indicate that this an abortive event between S 3 and regions. How ever,...
Inhibitor of NF-kappaB kinases beta (IKKbeta) and alpha (IKKalpha) activate distinct signaling modules. The IKKbeta/canonical pathway rapidly responds to stress-like conditions, whereas the IKKalpha/noncanonical controls adaptive immunity. Moreover, IKKalpha can attenuate IKKbeta-initiated inflammatory responses. High mobility group box 1 (HMGB1), a chromatin protein, is an extracellular signal tissue damage-attracting cells in inflammation, regeneration, scar formation. We show that IKKbeta...
A type III secretion system (T3SS) in pathogenic Yersinia species functions to translocate Yop effectors, which modulate cytokine production and regulate cell death macrophages. Distinct pathways of T3SS-dependent caspase-1 activation occur Yersinia-infected One pathway macrophages requires the effector YopJ. YopJ is an acetyltransferase that inactivates MAPK kinases IKKβ cause TLR4-dependent apoptosis naïve isoform Y. pestis KIM (YopJKIM) has two amino acid substitutions, F177L K206E, not...