A5010848619- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- SARS-CoV-2 and COVID-19 Research
- RNA regulation and disease
- Viral Infections and Immunology Research
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Light effects on plants
- Photoreceptor and optogenetics research
- Cancer Genomics and Diagnostics
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Rare Diseases
- Genomics and Phylogenetic Studies
- COVID-19 Impact on Reproduction
- Cancer therapeutics and mechanisms
- Chromosomal and Genetic Variations
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Glioma Diagnosis and Treatment
- Analog and Mixed-Signal Circuit Design
Palo Alto Institute
2022-2024
Stanford University
2021-2024
Arc Research Institute
2022-2024
Howard Hughes Medical Institute
2024
Salk Institute for Biological Studies
2018-2020
Broad Institute
2013-2018
McGovern Institute for Brain Research
2013-2016
Massachusetts Institute of Technology
2013-2016
INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive immune systems, which protect microbes against viruses other foreign DNA. All functionally characterized CRISPR systems have been reported to target DNA, with some multicomponent type III also targeting RNA. The putative class 2 VI system, has not characterized, encompasses the single-effector protein C2c2, contains two...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a range of symptoms in infected individuals, from mild illness to distress syndrome. A systematic understanding host factors influencing viral infection is critical elucidate SARS-CoV-2-host interactions and the progression Coronavirus disease 2019 (COVID-19). Here, we conducted genome-wide CRISPR knockout activation screens human lung epithelial cells with endogenous expression SARS-CoV-2 entry ACE2 TMPRSS2. We uncovered...
Abstract Genomic rearrangements, encompassing mutational changes in the genome such as insertions, deletions or inversions, are essential for genetic diversity. These rearrangements typically orchestrated by enzymes that involved fundamental DNA repair processes, homologous recombination, transposition of foreign material viruses and mobile elements 1,2 . Here we report IS110 insertion sequences, a family minimal autonomous elements, express structured non-coding RNA binds specifically to...
Insertion sequence (IS) elements are the simplest autonomous transposable found in prokaryotic genomes
Hormones mediate long-range cell communication and play vital roles in physiology, metabolism, health. Traditionally, endocrinologists have focused on one hormone or organ system at a time. Yet, signaling by its very nature connects cells of different organs involves crosstalk hormones. Here, we leverage the organism-wide single transcriptional atlas non-human primate, mouse lemur (Microcebus murinus), to systematically map source target for 84 classes This work uncovers...
Abstract The CRISPR-Cas adaptive immune system defends microbes against foreign genetic elements via DNA or RNA-DNA interference. We characterize the Class 2 type VI-A CRISPR-Caseffector C2c2 and demonstrate its RNA-guided RNase function. from bacterium Leptotrichia shahii provides interference RNA phage. In vitro biochemical analysis show that is guided by a single crRNA can be programmed to cleave ssRNA targets carrying complementary protospacers. bacteria, knock down specific mRNAs....
Identifying host genes essential for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has the potential to reveal novel drug targets and further our understanding of Disease 2019 (COVID-19). We previously performed a genome-wide CRISPR/Cas9 screen identify proviral factors highly pathogenic human coronaviruses. Few were required by diverse coronaviruses across multiple cell types, but DYRK1A was one such exception. Although its role in coronavirus infection undescribed, encodes D...
SUMMARY SARS-CoV-2 can cause a range of symptoms in infected individuals, from mild respiratory illness to acute distress syndrome. A systematic understanding the host factors mediating viral infection or restriction is critical elucidate host-pathogen interactions and progression COVID-19. To this end, we conducted genome-wide CRISPR knockout activation screens human lung epithelial cells with endogenous expression entry ACE2 TMPRSS2. These uncovered proviral antiviral across highly...
ABSTRACT Genomic rearrangements, encompassing mutational changes in the genome such as insertions, deletions, or inversions, are essential for genetic diversity. These rearrangements typically orchestrated by enzymes involved fundamental DNA repair processes homologous recombination transposition of foreign material viruses and mobile elements (MGEs). We report that IS110 insertion sequences, a family minimal autonomous MGEs, express structured non-coding RNA binds specifically to their...