A5087893982- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Bacterial Genetics and Biotechnology
- Cytomegalovirus and herpesvirus research
- Vibrio bacteria research studies
- Innovation and Socioeconomic Development
- Biochemical and Structural Characterization
- Evolution and Genetic Dynamics
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Streptococcal Infections and Treatments
- RNA Interference and Gene Delivery
- Bacterial Identification and Susceptibility Testing
- Botanical Research and Chemistry
- RNA regulation and disease
- Viral gastroenteritis research and epidemiology
- Genetics, Bioinformatics, and Biomedical Research
- Animal Genetics and Reproduction
- Biomedical and Engineering Education
- Antibiotic Use and Resistance
- Toxin Mechanisms and Immunotoxins
Howard Hughes Medical Institute
2018-2025
Rockefeller University
2016-2025
Sabin Vaccine Institute
2013
McGovern Institute for Brain Research
2013
New York Proton Center
2013
Northwestern University
2008-2010
Jane Coffin Childs Memorial Fund for Medical Research
2010
University of Chicago
2004-2008
Argonne National Laboratory
2005
Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different CRISPR/Cas systems demonstrate that Cas9 nucleases can be directed by RNAs induce cleavage at endogenous genomic loci in human mouse cells. also converted into a nicking...
Horizontal gene transfer (HGT) in bacteria and archaea occurs through phage transduction, transformation, or conjugation, the latter is particularly important for spread of antibiotic resistance. Clustered, regularly interspaced, short palindromic repeat (CRISPR) loci confer sequence-directed immunity against phages. A clinical isolate Staphylococcus epidermidis harbors a CRISPR spacer that matches nickase present nearly all staphylococcal conjugative plasmids. Here we show interference...
The ability to artificially control transcription is essential both the study of gene function and construction synthetic networks with desired properties. Cas9 an RNA-guided double-stranded DNA nuclease that participates in CRISPR-Cas immune defense against prokaryotic viruses. We describe use a mutant retains DNA-binding activity can be engineered as programmable repressor by preventing binding RNA polymerase (RNAP) promoter sequences or terminator blocking running RNAP. In addition,...
The immune systems that protect organisms from infectious agents invariably have a cost for the host. In bacteria and archaea CRISPR-Cas loci can serve as adaptive these microbes infectiously transmitted DNAs. When those DNAs are borne by lytic viruses (phages), this protection provide considerable advantage. immunity also prevent cells acquiring plasmids free DNA bearing genes increase their fitness. Here, we use combination of experiments mathematical-computer simulation models to explore...