A5033321160- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Image Processing Techniques and Applications
- Research Data Management Practices
- Photoacoustic and Ultrasonic Imaging
- Force Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Optical Coherence Tomography Applications
- Digital Holography and Microscopy
- Scientific Computing and Data Management
- Optical measurement and interference techniques
- Advanced Optical Imaging Technologies
- Optical Coatings and Gratings
- Tactile and Sensory Interactions
- Adaptive optics and wavefront sensing
- Neuroscience and Neural Engineering
- Lipid Membrane Structure and Behavior
- Integrated Circuits and Semiconductor Failure Analysis
- Interactive and Immersive Displays
- Advanced optical system design
- Calcium signaling and nucleotide metabolism
- Neural dynamics and brain function
- Optical Systems and Laser Technology
- Advancements in Photolithography Techniques
University of Exeter
2017-2024
University of Oxford
2011-2015
Science Oxford
2012-2015
Bellingham Technical College
2015
Qatar Cardiovascular Research Center
2013
Imperial College London
2013
University of Cambridge
2005-2007
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
The mangrove killifish, Krypotlebias marmoratus, is an intriguing vertebrate species, known for its unusual sexual systems. It one of two self-fertilizing simultaneous hermaphroditic vertebrates, alongside the related Kryptolebias ocellatus. While individuals can be born as primary males, this rare in wild and most are hermaphrodites, around 60% then turning into secondary males at 3 to 4 years age. K. marmoratus exceptionally tolerant harsh variable environmental conditions. This includes...
There is currently no widely adopted standard for the optical characterization of fluorescence microscopes. We used laser written to generate two- and three-dimensional patterns deliver a quick quantitative measure imaging performance. report on use two lateral resolution, illumination uniformity, lens distortion color plane alignment using confocal structured
Feeling the field! The intensity of second harmonic generation (SHG) from a porphyrin in lipid bilayer is exceptionally sensitive to electric field, thus indicating that these dyes may be useful probes for electrical signals excitable cells such as neurons. porphyrin-based membrane probe (see picture, not scale) gives fast electro-optic response which about 5–10 times greater than those conventional styryl dyes. Neurons communicate by using signals, mediated transient changes voltage across...
Sarcomere length (SL) is a key indicator of cardiac mechanical function, but current imaging technologies are limited in their ability to unambiguously measure and characterize SL at the cell level intact, living tissue.We developed method for measuring regional orientation using remote focusing microscopy, an emerging modality that can capture light from arbitrary oblique planes within sample.We present protocol quickly determines user-selected areas field view by 2 share common major axis...
It has previously been demonstrated in both simulation and experiment that well aligned remote focusing microscopes exhibit residual spherical aberration outside the focal plane. In this work, compensation of is provided by correction collar on primary objective, controlled a high precision stepper motor. A Shack-Hartmann wave front sensor used to demonstrate magnitude generated matches predicted an optical model objective lens. The limited impact diffraction range system described through...
Remote focusing (RF) is a technique that greatly extends the aberration-free axial scan range of an optical microscope. To maximise diffraction limited depth in RF system, magnification relay lenses should be such pupil planes objectives are accurately mapped on to each other. In this paper we study tolerance system mismatch and quantify amount residual spherical aberration present at different depths. We observe small deviations from ideal results increased amounts terms leading reduction...
Remote focussing microscopy allows sharp, in-focus images to be acquired at speed from outside of the focal plane an objective lens without any agitation specimen. However, careful optical alignment, advantages remote could compromised by introduction depth-dependent scaling artefacts. To achieve ideal alignment in a point-scanning microscope, lateral (XY) scan mirror pair must imaged onto back both reference and imaging objectives, telecentric arrangement. for many commercial lenses, it can...
Cardiac stimulation via sympathetic neurons can potentially trigger arrhythmias. We present approaches to study neuron-cardiomyocyte interactions involving optogenetic selective probing and all-optical electrophysiology measure activity in an automated fashion. Here we demonstrate the utility of optical interrogation their effects on macroscopic cardiomyocyte network dynamics address research targets such as adrenergic release neurotransmitters, effect neuronal numbers cardiac behavior,...
The extent to which holographic modal wavefront sensing can be applied the detection of ocular aberrations was investigated. First, idea extending dynamic range sensor by increasing mask bias and collection area pinhole detectors used in is reviewed. Errors single-mode owing reduced coherence from retinal scattering, photon, readout, quantization noise are evaluated. A sensitivity-to-noise metric introduced evaluate designs found maximized using a detector radius 8.6(flambda/NDelta) for...
Abstract— The development of a multi‐user stereoscopic display that does not require the use special glasses (autostereoscopic), and enables large degree freedom viewer movement requires only minimum amount information (a stereo pair) for displays described. optics comprise an RGB holographic laser projector is controlled by output multi‐target head‐position head tracker, optical assembly converts into steerable exit pupils, screen comprising single liquid‐crystal (LCD) image multiplexing...
We introduce a random-access parallel (RAP) imaging modality that uses novel design inspired by Newtonian telescope to image multiple spatially separated samples without moving parts or robotics. This scheme enables near-simultaneous capture of petri dishes and with sub-millisecond switching times at the full resolution camera. RAP system long-duration records from different in parallel, which is not possible using conventional automated microscopes. The demonstrated continuously cardiac...
1 - ABSTRACT Digital light microscopy provides powerful tools for quantitatively probing the real-time dynamics of subcellular structures. While power modern techniques is undeniable, rigorous record-keeping and quality control are required to ensure that imaging data may be properly interpreted ( ), reproduced reproducibility used extract reliable information scientific knowledge which can shared further analysis value ). Keeping notes on experiments procedures ought straightforward, as...
Abstract— The Multi‐User 3‐D Television Display (MUTED), designed to provide three‐dimensional television (3‐D TV) by the display of autostereoscopic imagery multiple viewers, each whom should enjoy freedom movement, is described. Such an system, which allows viewers simultaneously use head tracking, was previously demonstrated for TV applications in ATTEST project. However, requirement a dynamically addressable, steerable backlight presented several problems illumination source. MUTED...
The application of microscopy in biomedical research has come a long way since Antonie van Leeuwenhoek discovered unicellular organisms. Countless innovations have positioned light as cornerstone modern biology and method choice for connecting omics datasets to their biological clinical correlates. Still, regardless how convincing published imaging data looks, it does not always convey meaningful information about the conditions which was acquired, processed, analyzed. Adequate...
Die Intensität der Frequenzverdoppelung (SHG) durch ein Porphyrin in einer Lipiddoppelschicht wird außergewöhnlich stark von elektrischen Feldern beeinflusst. Daher könnten diese Farbstoffe nützliche Sonden für elektrische Signale anregbaren Zellen sein, z. B. Neuronen. porphyrinbasierte Membransonde (nicht maßstabsgetreu dargestellt) liefert eine schnelle elektrooptische Antwort, die ca. 5- bis 10mal stärker ist als bei herkömmlichen Styrylfarbstoffen.
Abstract An autostereoscopic display system, which allowed multiple viewers simultaneously by use of head‐tracking, was previously demonstrated for TV applications in the ATTEST project. However, requirement a dynamically addressable, movable backlight presented several problems illumination source. In this paper, authors demonstrate how novel laser‐based holographic projection system can be used to address these problems.
Fast confocal imaging was achieved by combining remote focusing with differential spinning disk optical sectioning to rapidly acquire images of live samples at cellular resolution. Axial and lateral full width half maxima less than 5 µm 490 nm respectively are demonstrated over 130 axial range a 256 × 128 field view. A water-index calibration slide used achieve an alignment that minimises image volume distortion. Application biological acquiring volumes 24 1 volume/s, allowing for the...
Abstract In the heart, endogenous nicotinic acid adenine dinucleotide phosphate (NAADP) triggers lysosomal calcium (Ca 2+ ) release to augment sarcoplasmic reticulum [1] Ca sequestration, producing larger transients. However, role of signals in pacemaker activity, a distinct -operated function sinoatrial node (SAN), or atrial myocardium has not been investigated. Pharmacological genetic ablation NAADP pathway inhibits spontaneous beating rate response β-adrenergic stimulation intact SAN. We...
<title>Abstract</title> In the heart, endogenous nicotinic acid adenine dinucleotide phosphate (NAADP) triggers lysosomal calcium release to augment sarcoplasmic reticulum (SR) sequestration, producing larger transients. However, role of signals in pacemaker activity, a distinct calcium-operated function sino-atrial node (SAN) or atria, function, has not been investigated. Pharmacological genetic ablation NAADP pathway inhibits spontaneous beating rate response β-adrenergic stimulation...