A5090740606- Advanced Fluorescence Microscopy Techniques
- Digital Holography and Microscopy
- Cell Image Analysis Techniques
- Image Processing Techniques and Applications
- Optical Coatings and Gratings
- Optical measurement and interference techniques
- Advancements in Photolithography Techniques
- Fluid Dynamics and Turbulent Flows
- Inorganic Fluorides and Related Compounds
- Thermography and Photoacoustic Techniques
- Photoreceptor and optogenetics research
- Electron and X-Ray Spectroscopy Techniques
- Surface and Thin Film Phenomena
- nanoparticles nucleation surface interactions
- Cold Atom Physics and Bose-Einstein Condensates
- Cardiac electrophysiology and arrhythmias
- Atmospheric Ozone and Climate
- Atomic and Molecular Physics
- Atmospheric aerosols and clouds
- Fluid Dynamics and Thin Films
- Luminescence Properties of Advanced Materials
- Surface Roughness and Optical Measurements
- Plasma and Flow Control in Aerodynamics
- Advanced Electron Microscopy Techniques and Applications
- Plasma Diagnostics and Applications
University of Glasgow
2023-2024
University of Exeter
2019-2021
Indian Institute of Astrophysics
2017
Indian Institute of Technology Bombay
2014
Sardar Patel University
1988-1990
The present work is concerned with the development and application of a novel fringe analysis technique based on principles windowed-Fourier-transform (WFT) for determination temperature concentration fields from interferometric images range heat mass transfer applications. Based extent noise level associated experimental data, has been coupled two different phase unwrapping methods: Itoh algorithm quality guided extraction. In order to generate experiments have carried out which include...
It has previously been demonstrated in both simulation and experiment that well aligned remote focusing microscopes exhibit residual spherical aberration outside the focal plane. In this work, compensation of is provided by correction collar on primary objective, controlled a high precision stepper motor. A Shack-Hartmann wave front sensor used to demonstrate magnitude generated matches predicted an optical model objective lens. The limited impact diffraction range system described through...
Remote focusing (RF) is a technique that greatly extends the aberration-free axial scan range of an optical microscope. To maximise diffraction limited depth in RF system, magnification relay lenses should be such pupil planes objectives are accurately mapped on to each other. In this paper we study tolerance system mismatch and quantify amount residual spherical aberration present at different depths. We observe small deviations from ideal results increased amounts terms leading reduction...
We introduce a random-access parallel (RAP) imaging modality that uses novel design inspired by Newtonian telescope to image multiple spatially separated samples without moving parts or robotics. This scheme enables near-simultaneous capture of petri dishes and with sub-millisecond switching times at the full resolution camera. RAP system long-duration records from different in parallel, which is not possible using conventional automated microscopes. The demonstrated continuously cardiac...
Fast confocal imaging was achieved by combining remote focusing with differential spinning disk optical sectioning to rapidly acquire images of live samples at cellular resolution. Axial and lateral full width half maxima less than 5 µm 490 nm respectively are demonstrated over 130 axial range a 256 × 128 field view. A water-index calibration slide used achieve an alignment that minimises image volume distortion. Application biological acquiring volumes 24 1 volume/s, allowing for the...
In the heart, action potentials travel through tissue to orchestrate muscle contraction and make organ an efficient pump. Scar tissues caused by myocardial infarction impairs electrical conduction. rabbit propagate from endocardium epicardium with a conduction velocity of 30 cm/s. Therefore, rapid vertical scan is necessary observe this transmural cardiac at cellular resolution. Here we present implementation versatile remote focusing module, compatible retrofitting commercial two-photon...
Remote focusing on a multiphoton microscope allows for fast axial scanning to track action potentials transmurally in slices of rabbit hearts. Here we present benchmark results cardiac structure and function.
ABSTRACT We apply a novel microscope architecture, the Exeter Multiscope, to problem of acquiring image data in rapid succession from nine wells 96 well plate. demonstrate that new can detect contraction cardiomyocyte monolayers which have been plated into these wells. Each is sampled using 500 x pixels across 1.4 mm field view, acquired three colours at 3.7 Hz per well. The use multiple illumination wavelengths provides post-hoc focus selection, further increasing level automation....
A comparative theoretical study has been done on the scattering of fast electrons by free (vapour) atoms and bound (solid) atoms, in particular, alkali Al Cu. The Born differential cross-sections (DCS), calculated with static plus polarization electronatom potential, are found general, to be larger for than at least small angles scattering. For Rb Cs two DCS tend merge very large only. sample incident energies chosen 400 eV above.
We introduce a solid state high throughput screening (ssHTS) imaging modality that uses novel Newtonian telescope design to image multiple spatially separated samples without moving parts or robotics. Conventional high-throughput modalities either require movement of the sample focal plane system 1–3 itself 4,5 , use wide-field approach capture several in one frame. Schemes which move can be mechanically complex and are inherently slow, while systems have poor light collection efficiency...
It has previously been demonstrated in both simulation and experiment that well aligned remote focusing microscopes exhibit residual spherical aberration outside the focal plane. In this work, compensation of is provided by correction collar on primary objective, controlled a high precision stepper motor. A Shack-Hartmann wave front sensor used to demonstrate magnitude generated matches predicted an optical model objective lens. The limited impact diffraction range system described through...