A5022894370- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Bone Tissue Engineering Materials
- Cardiac pacing and defibrillation studies
- Advanced Electron Microscopy Techniques and Applications
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Function and Risk Factors
- Chemotherapy-induced cardiotoxicity and mitigation
- Coronary Artery Anomalies
- Cardiac Arrhythmias and Treatments
- Cardiovascular Disease and Adiposity
- Cardiac Ischemia and Reperfusion
- Angiogenesis and VEGF in Cancer
- Lymphatic System and Diseases
- Body Contouring and Surgery
University of Glasgow
2016-2025
University of Nottingham
2023
University Medical Center Utrecht
2015
Electrical integration of human iPSC-derived cardiomyocyte (hiPSC-CM)-based tissue with the host myocardium is a requirement successful regeneration therapy. This study was designed to identify electrical coupling in acute phase (1-2h) post-grafting using an ex vivo model. Small, engineered heart tissues (mini-EHTs) consisting ∼50,000 hiPSC-CMs on hydrogel (spontaneous rate 0.34±0.05 Hz), were loaded Cal520-AM. EHTs implanted sub-epicardially into Langendorff-perfused rabbit after...
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) raise many possibilities for cardiac research but they exhibit an immature phenotype, which influences experimental outcomes. The aim of our is to investigate the effects a topographical gradient substrate on morphology and function commercially available hiPSC-CM. lateral dimensions microgrooves varied from 8 100 μm space between grooves one axis ∼5 nm ∼1 in depth other axis. Cells were seeded homogeneously across...
Arrhythmic sudden cardiac death (SCD) is an important cause of mortality following myocardial infarction (MI). The rabbit has similar electrophysiology to humans and therefore small animal model study post-MI arrhythmias. established approach surgical coronary ligation results in thoracic adhesions that impede epicardial electrophysiological studies. Adhesions are absent a percutaneously induced MI, which also associated with reduced morbidity so represents clear refinement the approach....
Abstract Biophysical cues robustly direct cell responses and are thus important tools for in vitro translational biomedical applications. High throughput platforms exploring substrates with varying physical properties therefore valuable. However, currently existing limited throughput, the biomaterials used, capability to segregate between different assessment of dynamic responses. Here we present a multiwell array (3 × 8) made substrate engineered topography or rigidity welded bottomless...
The lymphatic system plays a crucial role in interstitial fluid drainage, lipid absorption, and immunological defense. Lymphatic dysfunction results lymphedema, accumulation, swelling of soft tissues, as well potentially impaired immune response. Lymphedema significantly reduces quality life patients on physical, mental, social, economic basis. Current therapeutic approaches treatment disease are limited. Over the last decades, great progress has been made development strategies to enhance...
Abstract Human induced pluripotent stem cell‐derived cardiomyocytes (hiPSC‐CM) in monolayers interact mechanically via cell–cell and cell–substrate adhesion. Spatiotemporal features of contraction were analysed hiPSC‐CM (1) attached to glass or plastic (Young's modulus (E) >1 GPa), (2) detached (substrate‐free) (3) a flexible collagen hydrogel (E = 22 kPa). The effects isoprenaline on compared between rigid substrates. To clarify the underlying mechanisms, further gene expression...
In the heart, action potentials travel through tissue to orchestrate muscle contraction and make organ an efficient pump. Scar tissues caused by myocardial infarction impairs electrical conduction. rabbit propagate from endocardium epicardium with a conduction velocity of 30 cm/s. Therefore, rapid vertical scan is necessary observe this transmural cardiac at cellular resolution. Here we present implementation versatile remote focusing module, compatible retrofitting commercial two-photon...
Remote focusing on a multiphoton microscope allows for fast axial scanning to track action potentials transmurally in slices of rabbit hearts. Here we present benchmark results cardiac structure and function.
This video file contains fluoroscopic recordings of 6 steps the percutaneous inductions myocardial infarction in rabbit. 1. Identification aortic structures. 2. Insertion guide wire. 3. Guidance 4F catheter over 4. coronary ostium and apical artery. 5. Engagement vessel with a floppy 6. Advancement micro-catheter tip wire to deploy tip.
Abstract Biophysical cues robustly direct cell responses and are thus important tools for in vitro translational biomedical applications. High throughput platforms exploring substrates with varying physical properties therefore valuable, however, currently existing limited throughput, the biomaterials used, capability to segregate between different assessment of dynamic cellular responses. Here we present a multiwell array (3×8) using substrate engineered patterns that topography or rigidity...