A5029817784- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Ultrasonics and Acoustic Wave Propagation
- Cell Image Analysis Techniques
- Ultrasound Imaging and Elastography
- Acoustic Wave Phenomena Research
- Nanofabrication and Lithography Techniques
- Acoustic Wave Resonator Technologies
- Photoacoustic and Ultrasonic Imaging
- Semiconductor Lasers and Optical Devices
- Photonic Crystals and Applications
- Photonic and Optical Devices
- Cancer Cells and Metastasis
- Thermography and Photoacoustic Techniques
- Force Microscopy Techniques and Applications
- Immune cells in cancer
- Ultrasound and Hyperthermia Applications
- Mesenchymal stem cell research
- Ultrasound and Cavitation Phenomena
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Calibration and Measurement Techniques
- Pluripotent Stem Cells Research
- Advanced Biosensing Techniques and Applications
University of Glasgow
2015-2024
Glasgow Life
2016
Weidlinger Associates (United States)
2002-2010
University of Virginia
2004
University of Strathclyde
1998-2002
Łukasiewicz Research Network - Institute of Non-Ferrous Metals
1961-1964
Macrophages play a crucial rule in orchestrating immune responses against pathogens and foreign materials. have remarkable plasticity response to environmental cues are able acquire spectrum of activation status, best exemplified by pro-inflammatory (M1) anti-inflammatory (M2) phenotypes at the two ends spectrum. Characterisation M1 M2 subsets is usually carried out quantification multiple cell surface markers, transcription factors cytokine profiles. These approaches time-consuming, require...
Surface nanotopography is widely employed to control cell behavior and in particular controlled disorder has been shown be important differentiation/maturation. However, extracellular matrix proteins, such as fibronectin (FN), initially adsorbed on a biomaterial surface are known mediate the interaction of synthetic materials with cells. In this work, we examine effect through layer adhesive proteins using nanostructured polycarbonate comprising 150 nm-diameter pits originally defined...
It is emerging that mesenchymal stem cell (MSC) metabolic activity may be a key regulator of multipotency. The metabolome represents "snapshot" the phenotype, and therefore profiling could, through systems biology approach, offer highlight critical biochemical pathways for investigation. To date, however, it has remained difficult to undertake unbiased experiments study MSC multipotency in absence strategies retain without recourse soluble factors can add artifact experiments. Here we apply...
Abstract There is a rapidly growing body of literature on the effects topography and critically, nanotopography cell adhesion, apoptosis differentiation. Understanding adhesion morphology consequences shape changes in nucleus, consequently, gene expression offers new approaches to elucidation potential control stem In current study we have used molecular combination with immunohistology transcript analysis understand role mesenchymal phenotype. Results demonstrate large nucleus lamin...
Abstract Cells respond in complex ways to their environment, making it challenging predict a direct relationship between the two. A key problem is lack of informative representations parameters that translate directly into biological function. Here we present platform relate effects cell morphology gene expression induced by nanotopography. This utilizes ‘morphome’, multivariate dataset parameters. We create Bayesian linear regression model uses morphome robustly changes bone, cartilage,...
Abstract Mesenchymal stromal cells (MSCs) are multipotent progenitor that of considerable clinical potential in transplantation and anti-inflammatory therapies due to their capacity for tissue repair immunomodulation. However, MSCs rapidly differentiate once culture, making large-scale expansion use immunomodulatory challenging. Although the differentiation mechanisms have been extensively investigated using materials, little is known about how materials can influence paracrine activities...
Differentiation of human embryonic stem cells is widely studied as a potential unlimited source for cell replacement therapy to treat degenerative diseases such diabetes. The directed differentiation relies mainly on soluble factors. Although, some studies have highlighted that the properties physical environment, substrate stiffness, affect cellular behavior. Here, mass‐produced, injection molded polycarbonate nanopillars are presented, where surface mechanical properties, i.e., can be...
Nanoscale organisation of receptor ligands has become an important approach to study the clustering behaviour cell-surface receptors. Biomimetic substrates fabricated via different nanopatterning strategies have so far been applied investigate specific integrins and cell types, but without multivalent control. Here we use DNA origami surpass limits current approaches fabricate nanoarrays adhesion processes, with nanoscale spatial resolution single-molecule Notably, nanostructures enable...
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) raise many possibilities for cardiac research but they exhibit an immature phenotype, which influences experimental outcomes. The aim of our is to investigate the effects a topographical gradient substrate on morphology and function commercially available hiPSC-CM. lateral dimensions microgrooves varied from 8 100 μm space between grooves one axis ∼5 nm ∼1 in depth other axis. Cells were seeded homogeneously across...
Results are given for surfaces of roughness 3 μ in., 115 in. and anodized aluminium within the wavelength range 1 to 14 μ. The spectral emissivity varied strongly with surface oxidation, but only slightly temperature. Typical values at wavelengths 2 8 respectively were 0.09 0.04 polished, 0.30 0.24 roughened, 0.20 aluminium. Natural oxidation increased all below 9 had a marked effect between 10 12 An anodic oxide film beyond greater than 0.70. significance these variations radiation...
Experimental and theoretical evidence of silicon substrate ringing in microfabricated ultrasonic transducers is presented. This clearly observed immersion with a 650 /spl mu/m thick at 7 MHz harmonics. An analytical model the introduced, simulations based on are shown to agree experimental observation. results further compared carried out time-domain, large-scale PZFlex models qualitative agreement demonstrated. The insights gained from experiments used design fabricate device whose mode...
The function and fate of cells is influenced by many different factors, one which surface topography the support culture substrate. Systematic studies nanotopography cell response have typically been limited to single types a small set topographical variations. Here, we show radical expansion experimental throughput using automated detection, measurement, classification co-cultured on nanopillar array where feature height changes continuously from planar 250 nm over 9 mm. Individual are...
Abstract Cellular response to microgrooves is addressed using a new assay format, comprising orthogonal gradients of continuously varied groove pitch and depth. Dual layer etch masks are created combination micropatterning plasma polymer deposition. A silicon substrate with constant width 8 μm ridge increasing from in 0.5 steps across 10 mm fabricated by photolithography. plasma‐polymerized hexane film which 120 nm thick at one end these grooves, the other, deposited under diffusion mask....
The stiffness of the cardiovascular environment changes during ageing and in disease contributes to incidence progression. Changing collagen expression cross-linking regulate rigidity cardiac extracellular matrix (ECM). Additionally, basal lamina glycoproteins, especially laminin fibronectin cardiomyocyte adhesion formation, mechanics mechanosignalling. Laminin is abundant healthy heart, but increasingly expressed fibrotic heart. ECM receptors are co-regulated with changing ECM. Owing...
Flexible polymers such as poly dimethyl siloxane (PDMS) can be patterned at the micro- and nanoscale by casting, for a variety of applications. This replication-based fabrication process is relatively cheap fast, yet injection molding offers an even faster cheaper alternative to PDMS provided thermoplastic with similar mechanical properties used. In this paper, polyurethane evaluated its patterning ability aim forming type flexible structures used measure modulate contractile forces cells in...
Microelectromechanical systems (MEMS) electrostatic-based transducers inherently produce harmonics as the electrostatic force generated in transmit mode is approximately proportional to square of applied voltage signal. This characteristic precludes them from being effectively used for harmonic imaging (either with or without addition microbubble-based contrast agents). The signal that nonlinearly by tissue (or agent) cannot be distinguished inherent transmitted We investigated two...
Abstract Biophysical cues robustly direct cell responses and are thus important tools for in vitro translational biomedical applications. High throughput platforms exploring substrates with varying physical properties therefore valuable. However, currently existing limited throughput, the biomaterials used, capability to segregate between different assessment of dynamic responses. Here we present a multiwell array (3 × 8) made substrate engineered topography or rigidity welded bottomless...
A condition monitoring nondestructive evaluation (NDE) system, combining the generation of ultrasonic Lamb waves in thin composite plates and their subsequent detection using an embedded optical fiber system is described. The acoustic source low profile with respect to plate thickness, surface conformable, able efficiently launch a known wave mode, at operating frequencies between 100 500 kHz, over typical propagation distances mm. It incorporates both piezocomposite technology interdigital...
Abstract The finite size of 2D photonic crystals results in them being a lossy resonator, with the normally emitting modes conventional crystal surface lasers (PCSELs) differing photon lifetime via their different radiative rates, and in-plane losses higher order spatial modes. As consequence, fundamental mode (lowest loss) lowest out-of-plane scattering is primary lasing mode. For electrically driven PCSELs, as current increased, incomplete gain clamping additional (and spectral) leading to...
Micromachined ultrasonic transducer (MUT) arrays are topical because, among other opportunities, they may supplant piezoelectric in ultrasound medical imaging. However, imaging system companies need to explore performance and design issues thoroughly before making a serious commitment these silicon-based electrostatic devices. To this end, fully nonlinear, 3D MUT virtual prototyping capability was recently added PZFlex. It is described here applied medical-type arrays. Simple representations...
Standard methods for seeding monolayer cell cultures in a multiwell plate or dish do not uniformly distribute cells on the surface. With traditional methods, users find aggregation around circumference, centre, combination of two. This variation is introduced due to macro scale flow suspension, and movement before can settle attach Reproducibility between labs, users, experiments hampered by this variability seeding. We present simple method uniform user-independent using an easily produced...
We demonstrate the fabrication of sub-100 nm T-Gate structures using a single electron beam lithography exposure and tri-layer resist stack - PMMA/LOR/CSAR. Recent developments in modelling development were used to design process, which each is developed separately optimise resulting structure. By approach proximity correcting for full stack, we able independently vary gate length (50-100 nm) head size (250-500 at stage fabricate these T-Gates with high yield.
A three-dimensional non-linear model for simulating microbubble response to acoustic insonation is presented. 1 μm radius stimulated using positive and inverted 2.4 MHz pulses produced radius-time curves that matched (error <10%) with the experimental observation. bound 2.3 insonated 2.25 6 cycle pulse was observed oscillate max/min oscillations 45% lower than of free microbubble, this correlated (∼10% error) observations Garbin et al. [Appl. Phys. Lett. 90, 114103 (2007)]. The...