A5064268499- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Force Microscopy Techniques and Applications
- Cell Adhesion Molecules Research
- Nanofabrication and Lithography Techniques
- Polymer Surface Interaction Studies
- Erythrocyte Function and Pathophysiology
- Parasitic infections in humans and animals
- Tendon Structure and Treatment
- Cancer, Hypoxia, and Metabolism
- Textile materials and evaluations
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Polymer crystallization and properties
- Skin and Cellular Biology Research
- Microtubule and mitosis dynamics
- Neuroblastoma Research and Treatments
- Toxoplasma gondii Research Studies
- thermodynamics and calorimetric analyses
- Mesenchymal stem cell research
- Parasitic Infections and Diagnostics
- Rheology and Fluid Dynamics Studies
- Extracellular vesicles in disease
University of Glasgow
2021-2025
Barcelona Institute for Science and Technology
2025
Institute for Bioengineering of Catalonia
2025
Abstract Mesenchymal stromal cells (MSCs) are multipotent progenitor that of considerable clinical potential in transplantation and anti-inflammatory therapies due to their capacity for tissue repair immunomodulation. However, MSCs rapidly differentiate once culture, making large-scale expansion use immunomodulatory challenging. Although the differentiation mechanisms have been extensively investigated using materials, little is known about how materials can influence paracrine activities...
Abstract Laminins (LMs) are important structural proteins of the extracellular matrix (ECM). The abundance every LM isoform is tissue‐dependent, suggesting that has tissue‐specific roles. binds growth factors (GFs), which powerful cytokines widely used in tissue engineering due to their ability control stem cell differentiation. Currently, most commonly ECM mimetic material vitro Matrigel, a undefined composition containing and various GFs, but subjected batch variability lacking...
Abstract Extracellular matrix (ECM) viscoelasticity has emerged as a potent regulator of physiological and pathological processes, including cancer progression. Spatial confinement within the ECM is also known to influence cell behavior in these contexts. However, interplay between spatial driving epithelial mechanotransduction not well understood, it relies on experiments employing purely elastic hydrogels. This work presents an innovative approach fabricate micropattern viscoelastic...
<title>Abstract</title> Mechanosensitive ion channels, such as Piezo1, have emerged having fundamental roles in sensing the mechanical properties of extracellular matrix (ECM). However, whether and how Piezo1 senses time-dependent ECM properties, that is viscoelasticity, remains unknown. To address this question, we combined an immortalised mesenchymal stem cell (MSC) line which expression can be silenced with soft stiff viscoelastic hydrogels independently tuneable elastic viscous moduli....
Mesenchymal stem cells (MSCs) interact with their surroundings via integrins, which link to the actin cytoskeleton and translate physical cues into biochemical signals through mechanotransduction. N-cadherins enable cell-cell communication are also linked cytoskeleton. This crosstalk between integrins cadherins modulates MSC mechanotransduction fate. Here we show role of this in mechanosensing viscosity using supported lipid bilayers as substrates varying viscosity. We functionalize these...
Nanoindentation refers to a class of experimental techniques where micrometric force probe is used quantify the local mechanical properties soft biomaterials and cells. This approach has gained central role in fields mechanobiology, design tissue engineering, obtain proper characterization materials with resolution comparable size single cells (μm). The most popular strategy acquire such data employ an atomic microscope (AFM); while this instrument offers unprecedented (down pN) space...
Abstract In recent years, matrix viscoelasticity has emerged as a potent regulator of fundamental cellular processes and been implicated in promoting cancer progression. Alongside viscoelasticity, additional ECM cues have shown to influence migration decision-making cells, spatial confinement is now considered potential metastasis. However, our understanding these complex predominantly relies on purely elastic hydrogels, the exact relationship between driving epithelial cell...
The tumor microenvironment plays an important role in cancer development and the use of 3D vitro systems that decouple different elements this is critical for study progression. In neuroblastoma (NB), vitronectin (VN), extracellular matrix protein, has been linked to poor prognosis appears as a promising therapeutic target. Here, we developed hydrogels incorporate VN into polyethylene glycol (PEG) hydrogel networks recapitulate native NB microenvironment. stiffness VN/PEG was modulated be...
Abstract Cryptosporidiosis is a significant cause of diarrhoeal disease contributing to substantial morbidity and mortality for the immunocompromised young children, especially those who are malnourished. There no vaccines available effective treatments these patients. Another challenge that Cryptosporidia waterborne resistant common water including chlorination. transmitted as an oocyst made up hardy wall protects four parasites. Little understood about how constructed, its composition, it...
Abstract Mechanosensitive ion channels have emerged as fundamental proteins in sensing extracellular matrix (ECM) mechanics. Among those, Piezo1 has been proposed a key mechanosensor cells. However, whether and how senses time-dependent ECM mechanical properties (i.e., viscoelasticity) remains unknown. To address this question, we combined an immortalised mesenchymal stem cell (MSC) line with adjustable expression soft (400 Pa) stiff (25 kPa) viscoelastic hydrogels independently tuneable...
In article number 2010225, Manuel Salmeron-Sanchez and co-workers develop hydrogels that incorporate specific laminin isoforms. Laminins are ECM molecules tissue specific, promote integrin binding, sequester growth factors. The versatility of the system to engineer in vitro 3D cultures controlled stiffness mesenchymal stem cell differentiation (using LM 521) or neural 411) is demonstrated.
Nanoindentation refers to a class of experimental techniques where micrometric force probe is used quantify the local mechanical properties soft biomaterials and cells. This approach has gained central role in fields mechanobiology, design tissue engineering, obtain proper characterization materials with resolution comparable size single cells (μm). The most popular strategy acquire such data employ an atomic microscope (AFM); while this instrument offers unprecedented (down pN) space...