A5061644431- Force Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Nanofabrication and Lithography Techniques
- Microfluidic and Bio-sensing Technologies
- Fluid Dynamics and Mixing
- Monoclonal and Polyclonal Antibodies Research
- Blood properties and coagulation
- Minerals Flotation and Separation Techniques
- Neuroscience and Neural Engineering
- Single-cell and spatial transcriptomics
- Polymer Surface Interaction Studies
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biochemical and Structural Characterization
- Photoreceptor and optogenetics research
- Mechanical and Optical Resonators
- Photochromic and Fluorescence Chemistry
- Advanced Biosensing Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Receptor Mechanisms and Signaling
- nanoparticles nucleation surface interactions
- Immune Cell Function and Interaction
- Textile materials and evaluations
ETH Zurich
2018-2024
Institute for Biomedical Engineering
2018-2023
BioElectronics (United States)
2022
Biosensor (Italy)
2022
Board of the Swiss Federal Institutes of Technology
2020
Observatoire de la Côte d’Azur
2020
Université Côte d'Azur
2020
University of Florence
2020
University of Glasgow
2020
The mechanical properties of the skin determine tissue function and regulate dermal cell behavior. Yet measuring these remains challenging, as evidenced by large range elastic moduli reported in literature-from below one kPa to hundreds MPa. Here, we reconcile disparate results dedicated experiments at both cellular length scales computational models considering multiscale multiphasic structure. At macroscopic scale, collective behavior collagen fiber network under tension provides...
Abstract The dynamics of cellular membrane tension and its role in mechanosensing, which is the ability cells to respond physical stimuli, remain incompletely understood, mainly due lack appropriate tools. Here, we report a force-controlled nanopipette-based method that combines fluidic force microscopy with fluorescence imaging for precise manipulation while monitoring impact on single-cell mechanosensitivity. nanopipette enables control indentation imposed cell cortex as well aspiration...
Abstract Three‐dimensional (3D) control over the placement of bioactive cues is fundamental to understand cell guidance and develop engineered tissues. Two‐photon patterning (2PP) provides such at micro‐ millimeter scale, but nonspecific interactions between proteins functionalized extracellular matrices (ECMs) restrict its use. Here, a 2PP system based on nonfouling hydrophilic photocages Sortase A (SA)‐based enzymatic coupling presented, which offers unprecedented orthogonality...
Single-cell analysis is essential for uncovering heterogeneous biological functions that arise from intricate cellular interaction. Microfluidic droplet arrays enable precise dynamic data collection through cell encapsulation in picoliter volumes. The time-lapse imaging of these can reveal functional kinetics and fates, but accurate tracking identities across time frames remains challenging when droplets move significantly. Specifically, existing machine learning methods often depend on...
This study sheds light on a ground-breaking biochemical mechanotransduction pathway and reveals how Piezo1 channels orchestrate cell migration. We observed an increased migration rate in HEK293T (HEK) cells treated with Yoda1, agonist, or HEK overexpressing (HEK+P). Conversely, significant reduction motility was GsMTx4 (a channel inhibitor) upon silencing (HEK-P). Our findings establish direct correlation between alterations motility, expression, abnormal F-actin microfilament dynamics, the...
MicroRNA (miRNA) is a class of short RNA that emerging as an ideal biomarker, its expression level has been found to correlate with different types diseases including diabetes and cancer. The detection miRNA highly beneficial for early diagnostics disease monitoring. However, sensing remains difficult because small size low levels. Common techniques such quantitative real-time polymerase chain reaction (qRT-PCR), in situ hybridization Northern blotting have developed quantify given sample....
Nanoindentation refers to a class of experimental techniques where micrometric force probe is used quantify the local mechanical properties soft biomaterials and cells. This approach has gained central role in fields mechanobiology, design tissue engineering, obtain proper characterization materials with resolution comparable size single cells (μm). The most popular strategy acquire such data employ an atomic microscope (AFM); while this instrument offers unprecedented (down pN) space...
In recent years, cell-based assays have been frequently used in molecular interaction analysis. Cell-based complement traditional biochemical and biophysical methods, as they allow for analysis, mode of action studies even drug screening processes to be performed under physiologically relevant conditions. most cellular assays, biomolecules are usually labeled achieve specificity. order overcome some the drawbacks associated with label-based we recently introduced molography a biosensor...
Infections, autoimmune diseases, desired and adverse immunological responses to treatment can lead a complex dynamic cytokine response in vivo. This involves numerous immune cells secreting various cytokines orchestrate the reaction. However, secretion dynamics, amounts, co-occurrence of different by cell subtypes remain poorly understood due lack appropriate tools study them. Here, we describe protocol using microfluidic droplet platform that allows time-resolved quantitative measurement...
We established an in-plane microfabrication process of polymeric AFM micropipettes that include a microchannel with aperture at the apex without further processing after wafer scale lithography. The channel was obtained by etching sacrificial electrochemically plated copper layer. Such sideways fabrication scheme offers possibility designing various types cantilevers different shapes and lengths within single wafer, such as length 500 µm or being connected two triangular hollow tips. probes...
Infections, autoimmune diseases, desired and adverse immunological responses to treatment can lead a complex dynamic cytokine response in vivo. This involves numerous immune cells secreting various cytokines orchestrate the reaction. However, secretion dynamics, amounts, co-occurrence of different by cell subtypes remain poorly understood due lack appropriate tools study them. Here, we describe protocol using microfluidic droplet platform that allows time-resolved quantitative measurement...
Abstract Three-dimensional (3D) control over the placement of bioactive cues is fundamental to understand cell guidance and develop engineered tissues. Two-photon patterning (2PP) provides such at micro- millimeter scale, but non-specific interactions between proteins functionalized extracellular matrices (ECMs) restrict its use. Here we report a 2PP system based on non-fouling hydrophilic photocages Sortase A-based enzymatic coupling offering unprecedented orthogonality signal-to-noise...
Nanoindentation refers to a class of experimental techniques where micrometric force probe is used quantify the local mechanical properties soft biomaterials and cells. This approach has gained central role in fields mechanobiology, design tissue engineering, obtain proper characterization materials with resolution comparable size single cells (μm). The most popular strategy acquire such data employ an atomic microscope (AFM); while this instrument offers unprecedented (down pN) space...
In article number 1908299, Marcy Zenobi-Wong and co-workers introduce a novel two-photon patterning strategy based on enzymatic couplings, nonfouling hydrophilic photocages, improved protein functionalization reagents. Using time- spatially controlled laser exposure, precise anchoring of growth factors within hydrogel can guide the axons along biochemical paths. Illustration: Axon guided through maze by Riccardo Rizzo.
Abstract Background The mechanical properties of single living cells have proven to be a powerful marker the cell physiological state. use nanoindentation-based force spectroscopy provided wealth information on elasticity cells, which is still largely exploited. simplest model describe mechanics treat them as homogeneous elastic material and it in terms Young's modulus. Beside its simplicity, this approach proved extremely informative, allowing assess potential physical indicator towards...
Abstract Background: The mechanical properties of single living cells have proven to be a powerful marker the cell physiological state. use nanoindentation-based force spectroscopy provided wealth information on elasticity cells, which is still largely exploited. simplest model describe mechanics treat them as homogeneous elastic material and it in terms Young's modulus. Beside its simplicity, this approach proved extremely informative, allowing assess potential physical indicator towards...