We describe the fluidFM, an atomic force microscope (AFM) based on hollow cantilevers for local liquid dispensing and stimulation of single living cells under physiological conditions. A nanofluidic channel in cantilever allows soluble molecules to be dispensed through a submicrometer aperture AFM tip. The sensitive feedback controlled approach tip sample extremely modification surfaces environments. It also reliable discrimination between gentle contact with cell membrane or its...

Cell adhesion to surfaces represents the basis for niche colonization and survival. Here we establish serial quantification of forces different cell types using a single probe. The pace single-cell force-spectroscopy was accelerated up 200 yeast 20 mammalian cells per probe when replacing conventional trapping cantilever chemistry atomic force microscopy by underpressure immobilization with fluidic (FluidFM). In consequence, statistically relevant data could be recorded in rapid manner,...

Abstract The “ligand induced electroless plating (LIEP) process” is a simple process to obtain localized metal onto flexible polymers such as poly(ethylene terephtalate) and polyvinylidene fluoride sheets. This generic cost‐effective process, efficient on any common polymer surface, based the covalent grafting by GraftFast of thin chelating film, poly(acrylic acid), which can complex copper ions. entrapped ions are then chemically reduced in situ resulting Cu 0 species act seed layer for...

Abstract Alpha-synuclein (α-Syn) is a small presynaptic protein of 140 amino acids. Its pathologic intracellular aggregation within the central nervous system yields fibrillar inclusions named Lewy bodies that are hallmarks Parkinson’s disease (PD). In solution, pure α-Syn adopts an intrinsically disordered structure and assembles into fibrils exhibit considerable morphological heterogeneity depending on their assembly conditions. We recently established tightly controlled experimental...

Thermally responsive poly(N-isopropylacrylamide) (PNIPAM) films are attracting considerable attention since they offer the possibility to achieve reversible control over surface wettability and biocompatibility. In this paper, we first report a new simple method for grafting under melt of amine-terminated PNIPAM chains onto gold surfaces modified with self-assembled monolayer (SAM) reactive thiols. The formation homogeneous tethered films, whose thickness can be tuned by adjusting polymer...

Abstract Atomic force microscopy (AFM) investigations of living cells provide new information in both biology and medicine. However, slow cell dynamics the need for statistically significant sample sizes mean that data collection can be an extremely lengthy process. We address this problem by parallelizing AFM experiments using a two‐dimensional cantilever array, instead single cantilever. have developed instrument able to operate perform topographical mechanical air liquid. Deflection...

In this study, we present a lead-free, piezoelectric energy harvester capable of generating power in the milliwatt range. The consists four layers polyvinylidene difluoride polymer, bonded to cantilever with tip mass. cantilever’s resonance frequency was measured at 13.4 Hz. are connected parallel, resulting total capacitance 27 nF. At resonance, under open-circuit conditions, generates nearly 90 Vpp when subjected an acceleration 1 g. When impedance is matched, maximum output reaches 2.4...

The ligand induced electroless plating (LIEP) process was recently developed and thoroughly demonstrated with one of the most used polymers for processes: acrylonitrile-butadiene-styrene (ABS). This generic is based, thanks to use diazonium salts as precursors, on covalent grafting a thin layer poly(acrylic acid) (PAA) acting metallic onto pristine polymer surfaces. strategy takes advantage PAA ion exchange properties. Indeed, carboxylate groups contained in allow complex copper ions which...

A novel fabrication method was established to produce flexible, transparent, and robust tipless hollow atomic force microscopy (AFM) cantilevers made entirely from SU-8. Channels of 3 μm thickness several millimeters length were integrated into 12 thick 40 wide cantilevers. Connected a pressure controller, the devices showed high sealing performance with no leakage up 6 bars. Changing cantilever lengths 100 500 among same wafer allowed targeting various spring constants ranging 0.5 80 N m−1...

The preparation of ZnO thin films with controlled electrical resistivity and optical properties is often challenged by the presence defects, such as oxygen vacancies or interstitial zinc. Here, we investigate material polycrystalline prepared thermal Atomic Layer Deposition (ALD) molecular pulsing during growth. By means structural, characterizations, identify key growth parameters this unusual ALD process. Unexpectedly, influence molecules on crystallography, microstructure morphology...

Gallium-doped zinc oxide (GZO) films were fabricated using RF magnetron sputtering and atomic layer deposition (ALD). The latter ones demonstrate higher electrical conductivities (up to 2700 S cm–1) enhanced charge mobilities (18 cm2 V–1 s–1). morphological analysis reveals differences mostly due the very different nature of processes. film deposited via ALD shows an increased transmittance in visible range a small one infrared that leads figure merit 0.009 Ω–1 (10 times than for...

Energy harvesters are required for autonomous sensors. The field is still missing a low-cost technology that would provide enough power tangible low-frequency use cases, although triboelectric nanogenerators (TENG) have emerged as new energy technology. Standard vibration (VEHs) based either on bulky electromagnetic systems or stiff lead-based piezo-ceramics. Those rarely compatible with large deformations occur at low frequency such body movements. Here, we show stack of 10 thin layers...

Calculations of frequency modulation-atomic force microscopy (FM-AFM) images are presented. A virtual FM-AFM, which realistically simulates the experiment by including control system microscope, is implemented in order to go beyond usual static approximation. It shown that results obtained within approach can be recovered limit small scanning speed, while at realistic speed distorted. The influence experimental noise on investigated, allowing us evaluate sensitivity instrument.

This study proposes a tuning fork probe based nanomanipulation robotic system for mechanical characterization of ultraflexible nanostructures under scanning electron microscope. The force gradient is measured via the frequency modulation quartz and two nanomanipulators are used manipulation nanostructures. Two techniques proposed attaching nanostructure to tip probe. first technique involves gluing full range whereas second uses van der Waals electrostatic forces in order avoid destroying...

In order to characterize the mechanical behavior of fragile resonant microelectromechanical systems (MEMS)/nanoelectromechanical (NEMS), nondestructive measurements are required. this paper, a cartography local stiffness variations on suspended micromembrane is established for first time, by tuning-fork-based dynamic force sensor inside scanning electron microscope (SEM). Experiments conducted individually batch InP membranes 200 nm thin, using 9-degree-of-freedom (dof) nanomanipulation...

We report on the evidence of negative capacitance values in a system consisting metal-semiconductor-metal (MSM) structures, with Schottky junctions made zinc oxide thin films deposited by Atomic Layer Deposition (ALD) top platinum interdigitated electrodes (IDE). The MSM structures were studied over wide frequency range, between 20 Hz and 1 MHz. Light mechanical strain applied to device modulate positive or conductance characteristics tuning flow electrons involved conduction mechanisms. A...

We present the results of multiscale modeling process lateral manipulation a Pd adatom adsorbed on MgO (001) surface using noncontact atomic force microscope (AFM) at finite temperature and in real time as tip moves above surface. show that stochastic motion adatoms can be controlled by localized forces from an oscillating demonstrate how this achieved practice. The energy barriers for function position three dimensions are determined atomistic calculations then used kinetic Monte Carlo...

Atomic Force Microscopy (AFM) techniques are used with one- or two-dimensional arrays of piezoresistive probes for parallel imaging. We present a newly designed AFM platform to drive these passivated cantilever in air and liquid environments. Large area imaging as well qualitative quantitative analysis biological cells demonstrated by the means first time our knowledge. Noise limitations topography force resolutions piezolevers quantified.

Simultaneously inducing preferred crystalline orientation with a strong piezoelectric response in polycrystalline aluminum nitride (AlN) thin films by atomic layer deposition is technical challenge due to the upscaling of integration functionalities, such as sensing and actuation, micro-devices without any poling process. Utilizing low-temperature plasma-enhanced (PE-ALD), highly c-axis-oriented AlN have been prepared precise control over relative composition, purity levels, chemical states...

Polythiophene molecules adsorbed on a highly oriented pyrolytic graphite surface were studied by combined dynamic scanning tunneling microscopy (STM) and frequency modulation atomic force (FM-AFM) with quartz tuning fork sensor operating in Qplus mode equipped Pt/Ir tip. Upon completing careful sub-angström oscillation amplitude calibration of the probe, experiments conducted an ultra high vacuum at room temperature. By selecting tip/surface distance regulation parameter, one can select type...

We present the results of calculations performed to model process lateral manipulation an oxygen vacancy in MgO(001) surface using non-contact atomic force microscope (NC-AFM). The potential energy surfaces for as a function tip position are determined from atomistic modelling interacting with Mg terminated MgO tip. These energies then used dynamical evolution system oscillates and at finite temperature kinetic Monte Carlo method. is strongly dependent on temperature. It also found that...