A5088196352- 3D Printing in Biomedical Research
- Graphene and Nanomaterials Applications
- biodegradable polymer synthesis and properties
- Bone Tissue Engineering Materials
- Cellular Mechanics and Interactions
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Erythrocyte Function and Pathophysiology
- Lysosomal Storage Disorders Research
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA regulation and disease
- Cancer Cells and Metastasis
- Alzheimer's disease research and treatments
- RNA Research and Splicing
- Magnesium in Health and Disease
- Nanofabrication and Lithography Techniques
- Genetic Neurodegenerative Diseases
- RNA and protein synthesis mechanisms
- Phagocytosis and Immune Regulation
- Muscle Physiology and Disorders
- Galectins and Cancer Biology
- Polymer Surface Interaction Studies
- Blood properties and coagulation
University of Perugia
2016-2024
Regulatory volume decrease (RVD), a homeostatic process responsible for the re-establishment of original cell upon swelling, is critical in controlling several functions, including migration. RVD mainly sustained by swelling-activated Cl- current (ICl,swell ), which can be modulated cytoplasmic Ca2+ . Cell swelling also activates mechanosensitive channels, ubiquitously expressed -permeable channel Piezo1. We hypothesized that, and turn ICl,swell , Piezo1 involved fine regulation compared...
The association of lysosomal dysfunction and neurodegeneration has been documented in several neurodegenerative diseases, including Alzheimer's Disease (AD). Herein, we investigate the enzymes with AD at different stages progression disease (mild severe) or mild cognitive impairment (MCI). We conducted a screening two classes enzymes: glycohydrolases (β-Hexosaminidase, β-Galctosidase, β-Galactosylcerebrosidase, β-Glucuronidase) proteases (Cathepsins S, D, B, L) peripheral blood samples...
In the last decade, biopolymer matrices reinforced with nanofillers have attracted great research efforts thanks to synergistic characteristics derived from combination of these two components. this framework, review focuses on fundamental principles and recent progress in field aliphatic polyester-based nanocomposites for regenerative medicine applications. Traditional emerging polymer are described terms matrix properties synthesis methods, used nanofillers, nanocomposite processing...
Herein we present the production of novel nanocomposite films consisting polylactic acid (PLA) polymer and inclusion nanoparticles lignin (LNP), ZnO hybrid ZnO@LNP (ZnO, 3.5% wt, ICP), characterized by similar regular shapes different diameter distribution (30–70 nm 100–150 nm, respectively). The obtained set binary, ternary quaternary systems were in surface wettability morphology but tensile performance: while presence LNP PLA caused a reduction elastic modulus, stress deformation at...
This study sheds light on a ground-breaking biochemical mechanotransduction pathway and reveals how Piezo1 channels orchestrate cell migration. We observed an increased migration rate in HEK293T (HEK) cells treated with Yoda1, agonist, or HEK overexpressing (HEK+P). Conversely, significant reduction motility was GsMTx4 (a channel inhibitor) upon silencing (HEK-P). Our findings establish direct correlation between alterations motility, expression, abnormal F-actin microfilament dynamics, the...
Current knowledge indicates that the molecular cross-talk between stem cells and biomaterials guides cells' fate within a tissue engineering system. In this work, we have explored effects of interaction poly(l-lactide) acid (PLLA) polymer film human adult adipose (hASCs), focusing on events correlating materials' surface characteristics plasma membrane. hASCs were seeded films pristine PLLA modified by radiofrequency method under oxygen flow (PLLA+O₂). Comparative experiments performed using...
Herein, we have generated ssRNA aptamers to inhibit SARS-CoV-2 Mpro, a protease necessary for the coronavirus replication. Because there is no aptamer 3D structure currently available in databanks this protein, first, modeled an using entropic fragment-based strategy. We refined initial sequence and by two sequential approaches, consisting of elitist genetic algorithm RNA inverse process. identified three specific against called MAptapro, MAptapro-IR1, MAptapro-IR2, with similar...
Abstract Brillouin spectroscopy has recently attracted attention as a powerful tool for the characterization of mechanical properties heterogeneous materials, particularly in biological and biomedical domains. This study investigates procedure to use data provide relevant morphological parameters micro-structured samples. When acquiring spectra at interface between two regions sample, spectrum shows signatures both regions. feature can be used precisely identify position interfaces by...
The major challenge for stem cell translation regenerative medicine is the regeneration of damaged tissues by creating biological substitutes capable recapitulating missing function in recipient host. Therefore, current paradigm tissue engineering strategies combination a selected type, based on their capability to differentiate toward committed lineages, and biomaterial, that, due own characteristics (e.g., chemical, electric, mechanical property, nano-topography, nanostructured molecular...
The preparation of tailored nanomaterials able to support cell growth and viability is mandatory for tissue engineering applications. In the present work, silica nanoparticles were prepared by a sol-gel procedure then functionalized condensation amino groups adsorption silver nanoparticles. Transmission electron microscopy (TEM) imaging was used establish morphology average dimensions about 130 nm, which not affected functionalization. three samples deposited (1 mg/mL) on cover glasses, as...
The present work highlights the crucial role of interfacial compatibilization on design polylactic acid (PLA)/Magnesium (Mg) composites for bone regeneration applications. In this regard, an amphiphilic poly(ethylene oxide-b-L,L-lactide) diblock copolymer with predefined composition was synthesised and used as a new interface to provide physical interactions between metallic filler biopolymer matrix. This strategy allowed (i) overcoming PLA/Mg adhesion weakness (ii) modulating composite...
The biomedical translational applications of functionalized nanoparticles require comprehensive studies on their effect human stem cells. Here, we have tested neat star-shaped mesoporous silica (s-MSN) and chemically derivates; examined (NPs) with similar dimensions but different surface chemistry, due to the amino groups grafted (s-MSN-NH2), gold nanoseeds adsorbed (s-MSN-Au). samples were dropped glass coverslips obtain a homogeneous deposition differing only for NPs' chemical...
Herein, we present poly(butylene 1,4-cyclohexanedicarboxylate) (PBCE) films characterized by an unpatterned microstructure and a specific hydrophobicity, capable of boosting drastic cytoskeleton architecture remodeling, culminating with the neuronal-like differentiation human bone marrow-mesenchymal stem cells (hBM-MSCs). We have used two different filming procedures to prepare films, solvent casting compression-moulding (PBCE*). PBCE film had rough porous surface spherulite-like...
Abstract Background Cerebrospinal fluid (CSF) has emerged as a sensitive matrix for the screening of biomarkers diagnosis and clinical follow-up diseases with neurological manifestations, including some lysosomal storage disorders. In this study, we assessed range values arylsulfatase A (ARSA) activity in CSF pediatric adult donors, patients who underwent gene therapy metachromatic leukodystrophy (MLD). Methods cohort 56 samples was included study: donors (n = 36), 9), MLD 11) at different...
Herein, we explored the impact of lysosome dysfunction during progression Amyotrophic Lateral Sclerosis type-1 (ALS1). We conducted study in non-neural cells, primary fibroblasts (rFFFs), and bone marrow-mesenchymal stem cells (rBM-MSCs), isolated from animal model ratG93A for ALS1 at two stages disease: Pre-symptomatic-stage (ALS1-PreS) Terminal-stage (ALS1-EndS). documented storage human mutant Superoxide Dismutase 1, SOD1G93A (SOD1*) lysosomes ALS1-rFFFs ALS1-rBM-MSCs demonstrated...
Mechanotransduction is a molecular process by which cells translate physical stimuli exerted the external environment into biochemical pathways to orchestrate cellular shape and function. Even with advancements in field, events leading signal cascade are still unclear. The current biotechnology of tissue engineering offers opportunity study vitro effect biomaterial on stem mechanotransduction pathway involved process. Here, we cultured multipotent human mesenchymal/stromal (hMSCs) isolated...