A5093635788- Monoclonal and Polyclonal Antibodies Research
- Blood groups and transfusion
- T-cell and B-cell Immunology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Single-cell and spatial transcriptomics
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Complement system in diseases
- Bacterial Infections and Vaccines
- Advanced Biosensing Techniques and Applications
- Immune Cell Function and Interaction
- Air Quality Monitoring and Forecasting
- Combustion and flame dynamics
ETH Zurich
2024
Single-cell analysis is essential for uncovering heterogeneous biological functions that arise from intricate cellular interaction. Microfluidic droplet arrays enable precise dynamic data collection through cell encapsulation in picoliter volumes. The time-lapse imaging of these can reveal functional kinetics and fates, but accurate tracking identities across time frames remains challenging when droplets move significantly. Specifically, existing machine learning methods often depend on...
Infections, autoimmune diseases, desired and adverse immunological responses to treatment can lead a complex dynamic cytokine response in vivo. This involves numerous immune cells secreting various cytokines orchestrate the reaction. However, secretion dynamics, amounts, co-occurrence of different by cell subtypes remain poorly understood due lack appropriate tools study them. Here, we describe protocol using microfluidic droplet platform that allows time-resolved quantitative measurement...
Antibodies that trigger the complement system play a pivotal role in immune defense against pathogenic bacteria and offer potential therapeutic avenues for combating antibiotic-resistant bacterial infections, rising global concern. To gain deeper understanding of key parameters regulating activation by monoclonal antibodies, we developed novel bioassay quantifying classical at antibody level, employed this assay to characterize rare complement-activating antibacterial antibodies on...
Infections, autoimmune diseases, desired and adverse immunological responses to treatment can lead a complex dynamic cytokine response in vivo. This involves numerous immune cells secreting various cytokines orchestrate the reaction. However, secretion dynamics, amounts, co-occurrence of different by cell subtypes remain poorly understood due lack appropriate tools study them. Here, we describe protocol using microfluidic droplet platform that allows time-resolved quantitative measurement...