A5058345909- Parasitic Infections and Diagnostics
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Lysosomal Storage Disorders Research
- Viral gastroenteritis research and epidemiology
- Amoebic Infections and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Toxoplasma gondii Research Studies
- Toxin Mechanisms and Immunotoxins
- Sphingolipid Metabolism and Signaling
- Study of Mite Species
- CRISPR and Genetic Engineering
- Plant and Fungal Interactions Research
- Bacteriophages and microbial interactions
- Parasites and Host Interactions
- Cytomegalovirus and herpesvirus research
- Veterinary medicine and infectious diseases
- Liver Disease Diagnosis and Treatment
- Microbial infections and disease research
- Parasitic infections in humans and animals
Wellcome Centre for Anti-Infectives Research
2019-2024
University of Dundee
2019-2024
Texas Tech University
2012-2023
University of Georgia
2015-2019
Wellcome Trust
2019
The apicomplexan parasite Cryptosporidium is a leading global cause of severe diarrheal disease and an important contributor to early-childhood mortality. Waterborne outbreaks occur frequently, even in countries with advanced water treatment capabilities, there currently no fully effective treatment. Nucleotide pathways are attractive targets for antimicrobial development, several laboratories designing inhibitors these enzymes as potential infections. Here we take advantage newly available...
Abstract The apicomplexan parasite Cryptosporidium is a leading cause of diarrheal disease and an important contributor to overall global child mortality. We currently lack effective treatment immune prophylaxis. Recent advances now permit genetic modification this pathogen. expect produce rapid in fundamental as well translational research on cryptosporidiosis. Here we outline engineering for sufficient detail establish transfection any laboratory that requires access key technology. This...
Introduction Cryptosporidiosis is a leading cause of diarrheal-associated morbidity and mortality, predominantly affecting children under 5 years old in low-and-middle-income countries. There no effective treatment vaccine. New therapeutics are emerging from drug discovery efforts. It critical that mode action studies performed alongside to ensure the best clinical outcomes. Unfortunately, technology identify validate targets for Cryptosporidium severely lacking. Methods We used C. parvum...
Cryptosporidiosis is a diarrheal disease caused by infection with Cryptosporidium spp. parasites and leading cause of death in malnourished children worldwide. The only approved treatment, nitazoxanide, has limited efficacy this at-risk patient population. Additional safe therapeutics are urgently required to tackle unmet medical need. However, the development anti-cryptosporidial drugs hindered lack understanding optimal compound properties treat gastrointestinal infection. To address...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% total lipids Leishmania . PC synthesis mainly occurs via choline branch Kennedy pathway (choline ⇒ choline-phosphate CDP-choline PC) and N-methylation phosphatidylethanolamine (PE). In addition, parasites can acquire other from host or culture medium. this study, we assessed function essentiality ethanolamine phosphotransferase (CEPT) major which responsible for final step de novo PE. Our data...
is a leading cause of severe diarrhea and mortality in young children infants Africa southern Asia. More than twenty
Abstract Phosphatidylcholine (PC) is a major cell membrane constituent and precursor of important second messengers. In Leishmania parasites, PC synthesis can occur via the choline branch Kennedy pathway, N-methylation phosphatidylethanolamine (PE), or remodeling exogenous phospholipids. To investigate role de novo in , we focused on cholinephosphate cytidylyltransferase (CPCT) which catalyzes formation CDP-choline, key intermediate pathway. Without CPCT, L . parasites cannot incorporate...
Ethanolamine glycerophospholipids are ubiquitous cell membrane components. Trypanosomatid parasites of the genus Leishmania synthesize majority their ethanolamine as 1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine or plasmenylethanolamine (PME) through Kennedy pathway. PME is a subtype ether phospholipids also known plasmalogen whose functions not well characterized. In this study, we investigated role synthesis in major characterization an phosphotransferase (EPT) mutant. EPT-null...
Sphingosine kinase is a key enzyme in sphingolipid metabolism, catalysing the conversion of sphingosine or dihydrosphingosine into sphingosine-1-phosphate dihydrosphingosine-1-phosphate respectively. In mammals, powerful signalling molecule regulating cell growth, differentiation, apoptosis and immunity. Functions pathogenic protozoans are virtually unknown. While most organisms possess two closely related kinases, only one homologue (SKa) can be identified Leishmania, which vector-borne...
Glycerophospholipids including phosphatidylethanolamine (PE) and phosphatidylcholine (PC) are vital components of biological membranes. Trypanosomatid parasites the genus Leishmania can acquire PE PC via de novo synthesis uptake/remodeling host lipids. In this study, we investigated ethanolaminephosphate cytidylyltransferase (EPCT) in major , which is causative agent for cutaneous leishmaniasis. EPCT a key enzyme ethanolamine branch Kennedy pathway responsible PE. Our results demonstrate...
Glycerophospholipids including phosphatidylethanolamine (PE) and phosphatidylcholine (PC) are vital components of biological membranes. Trypanosomatid parasites the genus Leishmania can acquire PE PC via de novo synthesis uptake/remodeling host lipids. In this study, we investigated ethanolaminephosphate cytidyltransferase (EPCT) in major , which is causative agent for cutaneous leishmaniasis. EPCT a key enzyme ethanolamine branch Kennedy pathway responsible PE. Our results demonstrate that...
Abstract Cryptosporidiosis is a significant cause of diarrhoeal disease contributing to substantial morbidity and mortality for the immunocompromised young children, especially those who are malnourished. There no vaccines available effective treatments these patients. Another challenge that Cryptosporidia waterborne resistant common water including chlorination. transmitted as an oocyst made up hardy wall protects four parasites. Little understood about how constructed, its composition, it...
ABSTRACT Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% total lipids Leishmania . PC synthesis mainly occurs via choline branch Kennedy pathway (choline ⇒ choline-phosphate CDP-choline PC) and N-methylation phosphatidylethanolamine (PE). In addition, parasites can acquire other from host or culture medium. this study, we assessed function essentiality ethanolamine phosphotransferase (CEPT) major which responsible for final step de novo PE....