A5067493443- Parasitic Infections and Diagnostics
- Amoebic Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- IL-33, ST2, and ILC Pathways
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- Parasites and Host Interactions
- Eosinophilic Esophagitis
- RNA Research and Splicing
- Mycobacterium research and diagnosis
- Pneumocystis jirovecii pneumonia detection and treatment
Oregon Health & Science University
2024-2025
University of Pennsylvania
2018-2022
South University
2021
The apicomplexan parasite Cryptosporidium is a leading global cause of severe diarrheal disease and an important contributor to early-childhood mortality. Waterborne outbreaks occur frequently, even in countries with advanced water treatment capabilities, there currently no fully effective treatment. Nucleotide pathways are attractive targets for antimicrobial development, several laboratories designing inhibitors these enzymes as potential infections. Here we take advantage newly available...
The parasite Cryptosporidium is responsible for diarrheal disease in young children causing death, malnutrition, and growth delay. invades enterocytes where it develops a unique intracellular niche. Infected cells exhibit profound changes morphology, physiology, transcriptional activity. How the effects these poorly understood. We explored localization of highly polymorphic proteins found members parvum MEDLE protein family to be translocated into cytosol infected cells. All life stages...
Cryptosporidium is a leading cause of severe diarrhea and diarrheal-related death in children worldwide. As an obligate intracellular parasite, relies on intestinal epithelial cells to provide niche for its growth survival, but little known about the contributions that infected cell makes this relationship. Here we conducted genome wide CRISPR/Cas9 knockout screen discover host genes influence parvum infection and/or survival. Gene enrichment analysis indicated interferon response,...
To distinguish pathogens from commensals, the intestinal epithelium employs cytosolic innate immune sensors. Activation of NAIP–NLRC4 inflammasome initiates extrusion infected epithelial cells (IEC) upon bacterial sensing. We previously reported that activation in tuft cells, which are primarily known for their role parasitic infections, leads to release prostaglandin D2 (PGD2). observe an antibacterial response with increased IL-22 and antimicrobial protein levels within small intestine, is...
Abstract Intestinal epithelial cell (IEC) responses to interferon (IFN) favor antiviral defense with minimal cytotoxicity, but IEC-specific factors that regulate these remain poorly understood. Interferon regulatory (IRFs) are a family of nine related transcription factors, and IRF6 is preferentially expressed by cells, its roles in IEC immunity unknown. In this study, CRISPR screens found Irf6 deficiency enhanced IFN-stimulated transformed mouse IECs not macrophages. Furthermore, KO...
ABSTRACT The parasite Cryptosporidium is responsible for diarrheal disease in young children causing death, malnutrition, and growth delay. invades enterocytes where it develops a unique intracellular niche. Infected cells exhibit profound changes morphology, physiology transcriptional activity. How the effects these poorly understood. We explored localization of highly polymorphic proteins found members C. parvum MEDLE protein family to be translocated into cytoplasm infected cells. All...
SUMMARY The intestinal parasite, Cryptosporidium , is a major contributor to global child mortality and causes opportunistic infection in immune deficient individuals. Innate resistance which specifically invades enterocytes, dependent on the production of IFN-γ, yet whether enterocytes contribute parasite control poorly understood. In this study, utilizing natural mouse pathogen, tyzzeri we show that epithelial-derived IL-18 synergized with IL-12 stimulate innate lymphoid cell (ILC) IFN-γ....
Abstract Cryptosporidium is a leading cause of severe diarrhea and diarrheal-related death in children worldwide. As an obligate intracellular parasite, relies on intestinal epithelial cells to provide niche for its growth survival, but little known about the contributions that infected cell makes this relationship. Here we conducted genome wide CRISPR/Cas9 knockout screen discover host genes required parvum infection and/or survival. Gene enrichment analysis indicated interferon response,...