A5079265883- Trypanosoma species research and implications
- Parasitic Infections and Diagnostics
- Insect symbiosis and bacterial influences
- Research on Leishmaniasis Studies
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Biochemical and Molecular Research
- Coccidia and coccidiosis research
- Veterinary medicine and infectious diseases
- Parasitic Diseases Research and Treatment
- IL-33, ST2, and ILC Pathways
- Complement system in diseases
- Parasites and Host Interactions
- Calcium signaling and nucleotide metabolism
- Reproductive System and Pregnancy
University of Pennsylvania
2021-2024
University of Bern
2019-2022
South University
2021
Abstract The collective movement of African trypanosomes on semi-solid surfaces, known as social motility, is presumed to be due migration factors and repellents released by the parasites. Here we show that procyclic (insect midgut) forms acidify their environment a consequence glucose metabolism, generating pH gradients diffusion. Early late exhibit self-organising properties agarose plates. While early are repelled acid migrate outwards, remain at inoculation site. Furthermore, respond...
The unicellular parasite Trypanosoma brucei is transmitted between mammals by tsetse flies. Following the discovery that flagellar phosphodiesterase PDEB1 required for trypanosomes to move in response signals vitro (social motility), we investigated its role Here show knockout parasites exhibit subtle changes movement, reminiscent of bacterial chemotaxis mutants. Infecting flies with knockout, followed live confocal microscopy fluorescent within dual-labelled insect tissues, shows important...
Parasites and their hosts are engaged in reciprocal coevolution that balances competing mechanisms of virulence, resistance, evasion. This often leads to host specificity, but genomic reassortment between different strains can enable parasites jump barriers conquer new niches. In the apicomplexan parasite Cryptosporidium , genetic exchange has been hypothesized play a prominent role adaptation humans. The sexual lifecycle provides potential mechanism for such exchange; however, boundaries...
Generation of knockouts and in situ tagging genes Trypanosoma brucei has been greatly facilitated by using CRISPR/Cas9 as a genome editing tool. To date, this entailed limited number cell lines that are stably transformed to express Cas9 T7 RNA polymerase (T7RNAP). It would be desirable, however, able use for any trypanosome line.We describe sequential transfection expression system enables transient the two proteins, followed delivery PCR products gRNAs repair templates. This procedure can...
Cryptosporidium is an enteric pathogen and a prominent cause of diarrheal disease worldwide. Control requires CD4+ T cells, but how protective cell responses are generated poorly understood. Here, parasites that express MHCII-restricted model antigens were to understand the basis for priming effector function. These studies revealed parasite-specific cells primed in draining mesenteric lymph node differentiate into Th1 gut provide local parasite control. Although type 1 conventional...
Abstract Parasites and their hosts are engaged in rapid coevolution that balances competing mechanisms of virulence, resistance, evasion. This often leads to host specificity, but genomic reassortment between different strains can enable parasites jump barriers conquer new niches. In the apicomplexan parasite Cryptosporidium genetic exchange has been hypothesized play a prominent role adaptation humans. The sexual lifecycle provides potential mechanism for such exchange; however, boundaries...
Abstract Cryptosporidium is an enteric pathogen that a prominent cause of diarrheal disease. Control this infection requires CD4 + T cells, though the processes lead to cell-mediated resistance have been difficult assess. Here, parasites express MHCII-restricted model antigens were generated dissect early events influence cell priming and effector function. These studies highlight parasite-specific cells are primed in draining mesenteric lymph node (mesLN) differentiate into Th1 gut, where...
Cryptosporidium is a leading cause of severe diarrheal disease around the world.Recent field research points to parasite sex as source hyper-transmissibility fuelling continental sweeps, while new laboratory studies took advantage build powerful experimental tools understand and its interaction with host.In United States, was responsible for one largest recorded outbreaks, 1993 Milwaukee incident which infected more than 400,000 residents through contaminated drinking water.To this day half...
Abstract The collective movement of African trypanosomes on semi-solid surfaces, known as social motility, is presumed to be due migration factors and repellents released by the parasites. Here we show that procyclic (insect midgut) forms acidify their environment a consequence glucose metabolism, generating pH gradients diffusion. Early late exhibit self-organising properties agarose plates. While early are repelled acid migrate outwards, remain at inoculation site. Furthermore, respond...
SUMMARY The intestinal parasite, Cryptosporidium , is a major contributor to global child mortality and causes opportunistic infection in immune deficient individuals. Innate resistance which specifically invades enterocytes, dependent on the production of IFN-γ, yet whether enterocytes contribute parasite control poorly understood. In this study, utilizing natural mouse pathogen, tyzzeri we show that epithelial-derived IL-18 synergized with IL-12 stimulate innate lymphoid cell (ILC) IFN-γ....
Abstract Objective Generation of knockouts and in situ tagging genes Trypanosoma brucei has been greatly facilitated by using CRISPR/Cas9 as a genome editing tool. To date, this entailed limited number cell lines that are stably transformed to express Cas9 T7 RNA polymerase (T7RNAP). It would be desirable, however, able use for any trypanosome line. Results We describe sequential transfection expression system enables transient the two proteins, followed delivery PCR products gRNAs repair...
Abstract ObjectiveGeneration of knockouts and in situ tagging genes Trypanosoma brucei has been greatly facilitated by using CRISPR/Cas9 as a genome editing tool. To date, this entailed limited number cell lines that are stably transformed to express Cas9 T7 RNA polymerase (T7RNAP). It would be desirable, however, able use for any line.ResultsWe describe sequential transfection expression system enables transient the two proteins, followed delivery PCR products gRNAs repair templates. This...