A5008760336- Parasitic Infections and Diagnostics
- Sleep and Wakefulness Research
- Regulation of Appetite and Obesity
- Obstructive Sleep Apnea Research
- Clostridium difficile and Clostridium perfringens research
- Amoebic Infections and Treatments
- Toxoplasma gondii Research Studies
- Adipose Tissue and Metabolism
- Cytomegalovirus and herpesvirus research
- Rabies epidemiology and control
- Genomics and Phylogenetic Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Sleep and related disorders
- Mycobacterium research and diagnosis
- Viral Infections and Immunology Research
- Bartonella species infections research
- Circadian rhythm and melatonin
- Infective Endocarditis Diagnosis and Management
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Vector-borne infectious diseases
University of Pennsylvania
2018-2024
Texas A&M Health Science Center
2024
Texas A&M University
2024
South University
2019-2023
Western Kentucky University
2015-2018
Sleep deprivation induces acute inflammation and increased glucocorticosteroids in vertebrates, but effects from fragmented, or intermittent, sleep are poorly understood. Considering the latter is more representative of apnea humans, we investigated changes proinflammatory (IL-1β, TNF-α) anti-inflammatory (TGF-β1) cytokine gene expression periphery (liver, spleen, fat, heart) brain (hypothalamus, prefrontal cortex, hippocampus) a murine model exposed to varying intensities fragmentation...
Cryptosporidiosis is a leading cause of waterborne diarrheal disease globally and an important contributor to mortality in infants the immunosuppressed. Despite its importance, Cryptosporidium community has only had access good, but incomplete, parvum IOWA reference genome sequence. Incomplete sequences hamper annotation, experimental design, interpretation. We have generated new C. assembly supported by Pacific Biosciences (PacBio) Oxford Nanopore long-read technologies comparative...
The parasite Cryptosporidium is responsible for diarrheal disease in young children causing death, malnutrition, and growth delay. invades enterocytes where it develops a unique intracellular niche. Infected cells exhibit profound changes morphology, physiology, transcriptional activity. How the effects these poorly understood. We explored localization of highly polymorphic proteins found members parvum MEDLE protein family to be translocated into cytosol infected cells. All life stages...
Cryptosporidium is a leading cause of severe diarrhea and diarrheal-related death in children worldwide. As an obligate intracellular parasite, relies on intestinal epithelial cells to provide niche for its growth survival, but little known about the contributions that infected cell makes this relationship. Here we conducted genome wide CRISPR/Cas9 knockout screen discover host genes influence parvum infection and/or survival. Gene enrichment analysis indicated interferon response,...
Cryptosporidium causes debilitating diarrheal disease in patients with primary and acquired defects T cell function. However, it has been a challenge to understand how this infection generates responses they mediate parasite control. Here, was engineered express effector protein (MEDLE-2) that contains the major histocompatibility complex-I restricted SIINFEKL epitope which is recognized by receptor transgenic OT-I(OVA-TCR-I) clusters of differentiation (CD)8+ cells. These modified parasites...
Obligate intracellular bacteria have remained those for which effective vaccines are unavailable, mostly because protection does not solely rely on an antibody response. Effective antibody-based vaccines, however, been developed against extracellular pathogens or toxins. Additionally, obligate evolved many mechanisms to subvert the immune response, making vaccine development complex. Much of what we know about protective immunity these has determined using infection-resolved cases and animal...
Obesity and sleep fragmentation (SF) are often co-occurring pro-inflammatory conditions in patients with obstructive apnea. Leptin is a peptide hormone produced by adipocytes that has anorexigenic effects upon appetite while regulating immunity. The role of leptin mediating inflammatory responses to SF incompletely understood. Male C57BL/6j (lean) ob/ob mice (leptin-deficient exhibiting obese phenotype) were subjected or control for 24 h using an automated chamber. Trunk blood tissue samples...
Abstract Cryptosporidium causes debilitating diarrheal disease in patients with primary and acquired defects T cell function. However, it has been a challenge to understand how this infection generates responses they mediate parasite control. Here, was engineered express effector protein (MEDLE-2) that contains the MHC-I restricted SIINFEKL epitope which is recognized by TCR transgenic OT-I CD8 + cells. These modified parasites induced expansion of endogenous SIINFEKL-specific cells were...
ABSTRACT Cryptosporidiosis is a leading cause of waterborne diarrheal disease globally and an important contributor to mortality in infants the immunosuppressed. Despite its importance, Cryptosporidium community still relies on fragmented reference genome sequence from 2004. Incomplete sequences hamper experimental design interpretation. We have generated new C. parvum IOWA assembly supported by PacBio Oxford Nanopore long-read technologies comparative consistent annotation for three closely...
ABSTRACT The parasite Cryptosporidium is responsible for diarrheal disease in young children causing death, malnutrition, and growth delay. invades enterocytes where it develops a unique intracellular niche. Infected cells exhibit profound changes morphology, physiology transcriptional activity. How the effects these poorly understood. We explored localization of highly polymorphic proteins found members C. parvum MEDLE protein family to be translocated into cytoplasm infected cells. All...
Abstract Cryptosporidium is a leading cause of severe diarrhea and diarrheal-related death in children worldwide. As an obligate intracellular parasite, relies on intestinal epithelial cells to provide niche for its growth survival, but little known about the contributions that infected cell makes this relationship. Here we conducted genome wide CRISPR/Cas9 knockout screen discover host genes required parvum infection and/or survival. Gene enrichment analysis indicated interferon response,...